Tariq Mahmood
The Effect Of Different Levels Of Prostaglandin (Pgf 2 Alpha) In Extender On The Liveability Of Ram Spermatozoa - 1992
Pooled semen from six Lohi rams was extended in semen extender (Tris, Citric Acid, Fructose, Egg Yolk, Glycerol and Antibiotics). This extender was divided into four portions, four levels of prostaglanclin i.e. 0, 50, 100 and 150 ug/mI were added to the A, B, C and D Portions of extender respectively. These four levels ere compared on the basis of post thawing motility percentage, liveability (hours) of spermatozoa at 37°C and absolute index of liveability.
immediately after collection, the semen samples were examined for physical characteristics. Semen from each batch was filled in the plastic straws of four different colours (0.5 ml capacity), sealed with Polyvinyl Powder. Freezing was carried out in the liquid nitrogen at. -196°C for Storage.
Thawing of the frozen semen straws was performed at 35°C for 30 seconds. The average values of post thawing motility percentage were 36.25 ± 10.6, 45.62 ± 11.16, 55,62 ± 11.16 and 67.5 ± 7.55 for levels A,. B, C and D respectively. The values of liveablity for the four levels were 6.37 ± 1.68, 7.75 ± 2.02, 9 ± 1.85 and 11.25 2.18. hours respectively. The absolute index of liveablity averaged 12 .25 ± 67.28, 181.31 ± 85.62, 251.27 ± 104.56 and 382.5 ± 114.83 for levels A, B, C anti D respectively. The effect of' four levels on post thawing motility percentage, 1iveability (hours) and absolute index of liveability was significant. It was concluded that semen with 150 ug/mg prostaglandin produced encouraging results in terms of post thawing motility and liveablity as compared to 0, 50 and 100ug/ml Prostaglandin.
There is ample documentation that artificial insemination in sheep permits reproductive performance comparable to that obtained by natural mating. Better fertility results can be obtained by improvmenents in processing of semen for freezing, thawing temperature, time for thawing insemination technique and by adding different preparations like prostaglandins in semen.
Department of Theriogenology
0323,T
The Effect Of Different Levels Of Prostaglandin (Pgf 2 Alpha) In Extender On The Liveability Of Ram Spermatozoa - 1992
Pooled semen from six Lohi rams was extended in semen extender (Tris, Citric Acid, Fructose, Egg Yolk, Glycerol and Antibiotics). This extender was divided into four portions, four levels of prostaglanclin i.e. 0, 50, 100 and 150 ug/mI were added to the A, B, C and D Portions of extender respectively. These four levels ere compared on the basis of post thawing motility percentage, liveability (hours) of spermatozoa at 37°C and absolute index of liveability.
immediately after collection, the semen samples were examined for physical characteristics. Semen from each batch was filled in the plastic straws of four different colours (0.5 ml capacity), sealed with Polyvinyl Powder. Freezing was carried out in the liquid nitrogen at. -196°C for Storage.
Thawing of the frozen semen straws was performed at 35°C for 30 seconds. The average values of post thawing motility percentage were 36.25 ± 10.6, 45.62 ± 11.16, 55,62 ± 11.16 and 67.5 ± 7.55 for levels A,. B, C and D respectively. The values of liveablity for the four levels were 6.37 ± 1.68, 7.75 ± 2.02, 9 ± 1.85 and 11.25 2.18. hours respectively. The absolute index of liveablity averaged 12 .25 ± 67.28, 181.31 ± 85.62, 251.27 ± 104.56 and 382.5 ± 114.83 for levels A, B, C anti D respectively. The effect of' four levels on post thawing motility percentage, 1iveability (hours) and absolute index of liveability was significant. It was concluded that semen with 150 ug/mg prostaglandin produced encouraging results in terms of post thawing motility and liveablity as compared to 0, 50 and 100ug/ml Prostaglandin.
There is ample documentation that artificial insemination in sheep permits reproductive performance comparable to that obtained by natural mating. Better fertility results can be obtained by improvmenents in processing of semen for freezing, thawing temperature, time for thawing insemination technique and by adding different preparations like prostaglandins in semen.
Department of Theriogenology
0323,T