Rashid Hussain
Characterization Of Dgati Gene For K232A Polymorphism In Pakistani Cattle Breeds - 2011
Milk is a balanced diet because it contains all the essential nutrients like carbohydrates, fats, proteins, vitamins, minerals and enzymes required for health. In these milk nutrients fat is second most important component of milk. Primary component of milk fat is triglycerides (triacylglycerols or TAG), a typical storage form of lipids. DGAT1 gene has important role in milk fat percentage. This gene is located on the centromeric end of the bovine chromosome 14. It was recently studied that nonconservative dinucleotide (AA?GC) substitution in exon 8 of DGAT1 gene, change lysine to alanine at position 232 (K232A mutation) of the encoding protein. Animals that have K (Lysine) at position 232 in amino acid sequence of DGAT 1 will have high fat percentage and low milk yield as compared to animals that have A (Alanine) at this position. The objectives of this study were to identify K232A (Lysine232?Alanine) polymorphism in DGAT1 gene in Pakistani cattle breeds. To find relationship between milk production and K232A polymorphism. Blood samples were collected from different Govt. livestock farms/experimental stations. DNA was extracted by organic method. Specific primers were used for the amplification of exon8 of DGAT1 gene. After DNA amplification by Polymerase Chain Reaction, restriction digestion was done by using CfrI enzyme. Fourty animals belonging to Sahiwal and Dhanni breeds were genotyped for K232A polymorphism by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) technique. Sequencing was carried out to confirm the results of restriction digestion. From the data analyzed it was observed that all the animals of Sahiwal and Dhanni breeds had K allele and no A allele was identified. It has been shown that a missense mutation (Lys232 ? Ala) in the bovine DGAT1 gene in Pakistan cattle breeds is not common.
Institute of Biochemistry & Biotechnology
1283,T
Characterization Of Dgati Gene For K232A Polymorphism In Pakistani Cattle Breeds - 2011
Milk is a balanced diet because it contains all the essential nutrients like carbohydrates, fats, proteins, vitamins, minerals and enzymes required for health. In these milk nutrients fat is second most important component of milk. Primary component of milk fat is triglycerides (triacylglycerols or TAG), a typical storage form of lipids. DGAT1 gene has important role in milk fat percentage. This gene is located on the centromeric end of the bovine chromosome 14. It was recently studied that nonconservative dinucleotide (AA?GC) substitution in exon 8 of DGAT1 gene, change lysine to alanine at position 232 (K232A mutation) of the encoding protein. Animals that have K (Lysine) at position 232 in amino acid sequence of DGAT 1 will have high fat percentage and low milk yield as compared to animals that have A (Alanine) at this position. The objectives of this study were to identify K232A (Lysine232?Alanine) polymorphism in DGAT1 gene in Pakistani cattle breeds. To find relationship between milk production and K232A polymorphism. Blood samples were collected from different Govt. livestock farms/experimental stations. DNA was extracted by organic method. Specific primers were used for the amplification of exon8 of DGAT1 gene. After DNA amplification by Polymerase Chain Reaction, restriction digestion was done by using CfrI enzyme. Fourty animals belonging to Sahiwal and Dhanni breeds were genotyped for K232A polymorphism by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) technique. Sequencing was carried out to confirm the results of restriction digestion. From the data analyzed it was observed that all the animals of Sahiwal and Dhanni breeds had K allele and no A allele was identified. It has been shown that a missense mutation (Lys232 ? Ala) in the bovine DGAT1 gene in Pakistan cattle breeds is not common.
Institute of Biochemistry & Biotechnology
1283,T