Hadia Mubeen (2008-VA-291)
Seroprevalence And Molecular Detection Of Brucellosis In Animals In Mirpur, Azad Kashmir Pakistan - 2015. - 47p.;
Brucellosis is declared as one of the most widespread zoonoses in the world by the world's reknowned organizations. It is defined as a contagious systemic bacterial disease primarily of ruminants. The disease is manifested by late term abortions, weak calves, still births, infertility and also associated placentitis, epididymitis and orchitis, with excretion of the organisms in uterine discharges and milk. With the intensification of the import of animals and the establishment of big farms in the last few years, the incidence of brucellosis increased sharply in many countries, both in man and animals.
In this study 360 serum samples were examined from four groups of animals in district Mirpur Azad Kashmir. Blood samples of 3ml from buffaloes, cattle, sheep and goat (n=30) each were taken from three sub-divisions of Mirpur separately. The serum samples were screened by RBPT which is a screening test for brucellosis, and it was observed that 8.6% animals were seropositive by RBPT. The serum samples of cattle were 17.8%, buffalo were 8.9%, goat were 2.2%, and sheep were 5.6% positive respectively.
The serum samples positive by RBPT and some randomly taken samples were further confirmed by the use of most specific and sensitive serological test known as i-ELISA. 6.87% animals were confirmed as seropositive by i-ELISA including cattle (17.5%), buffalo (10%), sheep (0%) and goat (0%).
All RBPT positive samples were further subjected to RT-PCR. Among these 31 samples 24 were positive for Brucella genus and only 7 samples were negative. Samples were further tested for confirmation of Brucella species. All 24 samples were having Brucella abortus.
The data were analyzed by using the Statistical package for the social sciences (SPSS) program version 22. Data were analyzed using Chi-square test and Z-test statistics.
Department of Clinical Medicine
2378-T
Seroprevalence And Molecular Detection Of Brucellosis In Animals In Mirpur, Azad Kashmir Pakistan - 2015. - 47p.;
Brucellosis is declared as one of the most widespread zoonoses in the world by the world's reknowned organizations. It is defined as a contagious systemic bacterial disease primarily of ruminants. The disease is manifested by late term abortions, weak calves, still births, infertility and also associated placentitis, epididymitis and orchitis, with excretion of the organisms in uterine discharges and milk. With the intensification of the import of animals and the establishment of big farms in the last few years, the incidence of brucellosis increased sharply in many countries, both in man and animals.
In this study 360 serum samples were examined from four groups of animals in district Mirpur Azad Kashmir. Blood samples of 3ml from buffaloes, cattle, sheep and goat (n=30) each were taken from three sub-divisions of Mirpur separately. The serum samples were screened by RBPT which is a screening test for brucellosis, and it was observed that 8.6% animals were seropositive by RBPT. The serum samples of cattle were 17.8%, buffalo were 8.9%, goat were 2.2%, and sheep were 5.6% positive respectively.
The serum samples positive by RBPT and some randomly taken samples were further confirmed by the use of most specific and sensitive serological test known as i-ELISA. 6.87% animals were confirmed as seropositive by i-ELISA including cattle (17.5%), buffalo (10%), sheep (0%) and goat (0%).
All RBPT positive samples were further subjected to RT-PCR. Among these 31 samples 24 were positive for Brucella genus and only 7 samples were negative. Samples were further tested for confirmation of Brucella species. All 24 samples were having Brucella abortus.
The data were analyzed by using the Statistical package for the social sciences (SPSS) program version 22. Data were analyzed using Chi-square test and Z-test statistics.
Department of Clinical Medicine
2378-T