Sonia Akram (2009-VA-160)
Effect Of Lactobacillus Bulgaricus On Cutaneous Wound Healing In Mice - 2016. - 67p.;
Integrity loss of major part of the skin as a result of injury, surgery, or sickness can lead to a major disability or even death. The major complication which comes in healing process is infection. Besides reports of side effects, a diversity of chemical agents is in use to facilitate wound healing process. Use of probiotics for the purpose is an approach of altering the microbial environment of wound where beneficial bacteria compete with pathogenic bacteria. Lactobacillus bulgaricus extracellular polymeric substance (EPS) contains high levels of phosphate groups which help activate macrophages and lymphocytes.
Lactobacillus bulgaricus was isolated from sources like: homemade curd, yogurt, and commercial probiotic product (Protexin, UK) as per WHO guideline. For bacterial identification, the isolates were further characterized using biochemical tests. Male mice (n= 60) were kept and divided into four groups having 15 mice in each group (A to D). Experimental treatments using suspension of Lactobacillus bulgaricus, pure honey and sterile normal saline were applied to surgically created wounds on skin of mice belonging to three groups (A, B, and C), whereas, one group (D) was left untreated. After application of treatments, tissue samples were collected from these groups, processed and examined for histopathology. Increase in number of macrophages, neutrophils and fibroblasts at wound site of mice of all groups, was recorded during histopathological examination of H & E stained slides. Results were tabulated using one-way ANOVA.
Experimental groups A and B (L. bulgaricus and honey) showed significant increase in the cells as compared to control and negative control groups (C and D). L. bulgaricus treated group
CHAPTER 6
SUMMARY
Summary
61
showed marked healing of cutaneous wound than control and negative control groups. It was observed that in L. bulgaricus treated group there was marked increase in healing process as inflammation phase of wound passed quickly. Whereas, when healing efficacy of Lactobacillus bulgaricus and honey treated groups was compared, honey was efficacy of cutaneous wound healing was significantly more than Lactobacillus bulgaricus in terms of wound contraction and subsiding inflammation. It is concluded that holistically use of honey for wound healing in mice is more efficacious therapy than use of Lactobacillus bulgaricus suspension.
Microbiology
2597-T
Effect Of Lactobacillus Bulgaricus On Cutaneous Wound Healing In Mice - 2016. - 67p.;
Integrity loss of major part of the skin as a result of injury, surgery, or sickness can lead to a major disability or even death. The major complication which comes in healing process is infection. Besides reports of side effects, a diversity of chemical agents is in use to facilitate wound healing process. Use of probiotics for the purpose is an approach of altering the microbial environment of wound where beneficial bacteria compete with pathogenic bacteria. Lactobacillus bulgaricus extracellular polymeric substance (EPS) contains high levels of phosphate groups which help activate macrophages and lymphocytes.
Lactobacillus bulgaricus was isolated from sources like: homemade curd, yogurt, and commercial probiotic product (Protexin, UK) as per WHO guideline. For bacterial identification, the isolates were further characterized using biochemical tests. Male mice (n= 60) were kept and divided into four groups having 15 mice in each group (A to D). Experimental treatments using suspension of Lactobacillus bulgaricus, pure honey and sterile normal saline were applied to surgically created wounds on skin of mice belonging to three groups (A, B, and C), whereas, one group (D) was left untreated. After application of treatments, tissue samples were collected from these groups, processed and examined for histopathology. Increase in number of macrophages, neutrophils and fibroblasts at wound site of mice of all groups, was recorded during histopathological examination of H & E stained slides. Results were tabulated using one-way ANOVA.
Experimental groups A and B (L. bulgaricus and honey) showed significant increase in the cells as compared to control and negative control groups (C and D). L. bulgaricus treated group
CHAPTER 6
SUMMARY
Summary
61
showed marked healing of cutaneous wound than control and negative control groups. It was observed that in L. bulgaricus treated group there was marked increase in healing process as inflammation phase of wound passed quickly. Whereas, when healing efficacy of Lactobacillus bulgaricus and honey treated groups was compared, honey was efficacy of cutaneous wound healing was significantly more than Lactobacillus bulgaricus in terms of wound contraction and subsiding inflammation. It is concluded that holistically use of honey for wound healing in mice is more efficacious therapy than use of Lactobacillus bulgaricus suspension.
Microbiology
2597-T