Studies Of The Production And Detection Of Haemolytic Toxin In In Vitro Culture Of Clostridium Perfringens Type D (Record no. 2439)
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| fixed length control field | 01711nam a2200205Ia 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20150918160627.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 150525s1998 xx 000 0 und d |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 0698,T |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Bakht Sultan |
| 110 ## - MAIN ENTRY--CORPORATE NAME | |
| Location of meeting | Dr . Khushi Muhammad |
| 245 ## - TITLE STATEMENT | |
| Title | Studies Of The Production And Detection Of Haemolytic Toxin In In Vitro Culture Of Clostridium Perfringens Type D |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Year of publication | 1998 |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | Physicochemical factors modulating the production of haemolysin in culture of Clostridium perfringens (type-D) were evaluated. It was observed that toxin was produced in all the three media. The maximum titer of (4184) was achieved in RCM. The titer in thioglycollate was 2088 and in RCM with K2HPO4 were 1248 after 24 hours incubation. It was observed that pH 6.5, 7.0 and 7.5 of the medium before incubation resulted 1024, 4184 and 1576 haemolytic titers. Anaerobic environment and neutral pH during incubation augmented the haemolysin production in the culture. Trypsin 0.1 percent in the culture filtrate converted the prototoxin into haemolysin which exhibited maximum lytic activity in 60 minutes interaction time. Trypsin solution (1 percent) alone failed to induce haemolysis while the haemolysin showed maximum haemolytic activity at 37°C. The trypsinised culture supernatant (haemolysin) induced lysis of erythrocytes of sheep, goat, horse and chicken. The resultant high titer of haemolysin unveiled the propects of preparation of combined vaccines for sheep and goats. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Department of Microbiology |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dr . Haji Ahmad |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dr . Sameera Akhtar |
| 710 ## - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Faculty of Veterinary Sciences |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Thesis |
| Damaged status | Collection code | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Accession Number | Koha item type |
|---|---|---|---|---|---|---|---|---|
| Veterinary Science | UVAS Library | UVAS Library | Thesis Section | 2015-05-27 | 0698,T | 0698,T | Thesis |