Preparation And Evaluation Of Newcastle Disease Virus (Mesogenic Strain) Oil Based Vaccine (Record no. 2441)
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| fixed length control field | 02122nam a2200205Ia 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20150918160716.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 150525s1998 xx 000 0 und d |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 0700,T |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Shafi Ullah Chand |
| 110 ## - MAIN ENTRY--CORPORATE NAME | |
| Location of meeting | Dr . Khushi Muhammad |
| 245 ## - TITLE STATEMENT | |
| Title | Preparation And Evaluation Of Newcastle Disease Virus (Mesogenic Strain) Oil Based Vaccine |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Year of publication | 1998 |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | The present work was proposed to prepare oil based Newcastle disease virus vaccine and to compare its efficacy with imported vaccines. An oil based ND vaccine was prepared using moderately virulent strain of NDV. The virus was cultured in chicken embryos. The allanto-amniotic fluid, chorioallantoic membrane and infected embryo (virus suspension) was subjected to titration. The HA titer of allanto-amniotic fluid (AAF), chorloallantoic membrane (CAM) and embryo was upto 512, 1024 and 2048, respectively. The MD50 was calculated to be 1088/0.1ml. Effect of temperature on its keeping quality was determined by estimation of its HA potential at various intervals. The AAF was processed for inactivation, sterility and safety tests. Formalin at a rate of 0.12% inactivated the NDV in 48 hours at 37°C. Addition of antibiotic such as gentarnycin and nystatin inhibited common contaminants. An oil based NDV vaccine was prepared by mixing one part of processed AAF in 4 parts of oil base. The oil base contained 4% emulsifier span-80 and 1% tween-80. The vaccine thus prepared from moderately virulent strain was antigenically comparable with the imported ND vaccine. The cost of vaccine production using moderately virulent NDV was Rs.463/bottle (1000 doses) compared to RS.1250/- per bottle of imported vaccine. The price of one ml diagnostic antigen was calculated at Rs.2/ml. The results of present project encourage to develop an economical and effective oil based ND vaccine and diagnostic HA NDV antigen. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Department of Microbiology |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dr . Sameera Akhtar |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Dr . Shakil |
| 710 ## - ADDED ENTRY--CORPORATE NAME | |
| Corporate name or jurisdiction name as entry element | Faculty of Veterinary Sciences |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Thesis |
| Damaged status | Collection code | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Accession Number | Koha item type |
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| Veterinary Science | UVAS Library | UVAS Library | Thesis Section | 2015-05-27 | 0700,T | 0700,T | Thesis |