| 000 -LEADER |
|---|
| fixed length control field |
02184nam a2200205Ia 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
|---|
| control field |
20150921125912.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
|---|
| fixed length control field |
150525s1985 xx 000 0 und d |
| 041 ## - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER |
|---|
| Classification number |
0703,T |
| 100 ## - MAIN ENTRY--AUTHOR NAME |
|---|
| Personal name |
Tehmina Sadaf |
| 110 ## - MAIN ENTRY--CORPORATE NAME |
|---|
| Location of meeting |
Dr . Sameera Akhtar |
| 245 #2 - TITLE STATEMENT |
|---|
| Title |
A Study On Physico Chemical Actors Affecting The Survial Of Avian Influenza (H9 N2) Virus |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Year of publication |
2001 |
| 502 ## - DISSERTATION NOTE |
|---|
| Dissertation note |
A total of 310 chicken embryos (9 day old) were purchased from local market. The embryos were incubated at 37°C. On eleventh day of age avian influenza virus (H9 N2) for its propagation was inoculated in 10 chicken embryos. The four haemagglutination titer (4HA) of the virus was prepared to observe the response to various physical and chemical factors. Physical factors included were temperature, pH and UV (280 nm) light. The virus, exposed to the physical factors for different time intervals was inoculated into embryos through allantoic route. These embryos were kept in an incubator (37°C) for 72 hours. Later on the allantoic amniotic fluid (AAF) from each inoculated embryos subjected to spot haemagglutination test. The virus endured 56 °C temperature for 15 and 30 minutes while got inactivated in 45 minutes. More over virus survived at pH 7 for 15, 30 and 45 minutes but lost its HA activity at pH 5 and 9 in 15, 30 and 45 minutes. It was further examined that virus survived after 60 minutes exposure UV light but inactivated after 90 minutes. The disinfectants formalin, phenol, iodine solution and fin virus were used in 0.5%, 1% and 1.5% concentrations. The 4HA titer of virus was mixed in various concentrations of chemical disinfectants and was inoculated into embryonated eggs. The AAF of these eggs was subjected to spot agglutination test. The results of the test showed that all four chemicals formalin, phenol, iodine and fin virus inactivated the virus in 0.5% concentration in 15 minutes and all have good antiviral activity against avian influenza virus.
|
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical Term |
Department of Microbiology |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Dr . Khushi Muhammad |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Dr . Shakil |
| 710 ## - ADDED ENTRY--CORPORATE NAME |
|---|
| Corporate name or jurisdiction name as entry element |
Faculty of Veterinary Sciences |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Thesis |
