Genotyping Of Echinococcus Granulosus And Its Comparative Prevalance In Camels And Human Beings (Record no. 2732)

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fixed length control field 03117nam a2200205Ia 4500
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20170802132911.0
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fixed length control field 150525s2008 xx 000 0 und d
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 1010,T
100 ## - MAIN ENTRY--AUTHOR NAME
Personal name Azam Ali
110 ## - MAIN ENTRY--CORPORATE NAME
Location of meeting Prf.Dr. Azhar Maqbool
245 ## - TITLE STATEMENT
Title Genotyping Of Echinococcus Granulosus And Its Comparative Prevalance In Camels And Human Beings
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Year of publication 2008
502 ## - DISSERTATION NOTE
Dissertation note Hydaiidosis is caused by metacestode of the dog worm Echinococcus granulosus. It is a serious problem br both Public health and livestock economy. Echinococcus granaiiosu.s has number of genetically distinct strains which are known to differ morphologically and epidemiologically. Out of 100 camels examined only 25 Samples of hydatid cysts were collected from different organs i.e. livers, kidneys, lungs and hearts from Lahore abattoirs. Fertility and viability of the cysts was observed microscopically. Genotyp ing of Echinococcus granulosus was performed through Polymerase Chain Reaction Restriction Fragment Length Polymorphism (PCR-RFLP). Seroprevalence of hydatidosis in 25 butchers working in abattoir was also determined by the use of Latex agglutination test (LAT) kit for detection ob hydatidosis.
Considerable information is available about genetic variants of E. granulosus around the world. Ten genotypes of E. granulosus have been described, which exhibit a diversity of morphology, development, and host range, as contrmed by various studies. In the Mediterranean area, the CI or common sheep strain, G2, Camel strain G6, and the equine strain G4 have been found in Spain, Italy, Lebanon, and Syria
To date, molecular studies using mainly DNA sequences have identitied G-6 strain of E. granulosus. This categorization follows very closely the patterns of strain variation emerging from biological and epiderniological traits.
In this study we perform serum analysis of butchers to detect antibodies against Echinococcus so that the prevalence of Echinococcus can be checked; the data available indicated that 14% of butcher's population is infected with Echinococcus. In order to confirm the strain of Echinococcus in camels the PCR-RFLP analysis were performed. The data obtained was analysed and it was concluded that the G6 strain of Echinococciis is prevalent in camels in Pakistan. The results demonstrated that PCRRFLP analysis of samples of patients suspected for Echinococcus is a promising diagnostic method and also confirms the type of Echinococcus prevalent in that area and also enables an early direct detection of parasite DNA. This will help to curtail this drastic malady at an early stage and will help to devise the strategy to minimize the losses due to this disease.
It is hoped that the findings of the present study will be helpful for further planning about the control of the disease and correlating the prevalence in camels and butchers from the zoonotic point of view.
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Department of Parasitology
650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical Term Phd. Theses
700 ## - ADDED ENTRY--PERSONAL NAME
Personal name Dr.Kamran Ashraf
710 ## - ADDED ENTRY--CORPORATE NAME
Corporate name or jurisdiction name as entry element Faculty of Veterinary Sciences
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type Thesis
Holdings
Damaged status Collection code Permanent Location Current Location Shelving location Date acquired Full call number Accession Number Koha item type
  Veterinary Science UVAS Library UVAS Library Thesis Section 2015-05-27 1010,T 1010,T Thesis


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