| 000 -LEADER |
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| fixed length control field |
02147nam a2200193Ia 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20151005152347.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
150525s2012xx 000 0 und d |
| 041 ## - LANGUAGE CODE |
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| Language code of text/sound track or separate title |
eng |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER |
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| Classification number |
1393,T |
| 100 ## - MAIN ENTRY--AUTHOR NAME |
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| Personal name |
Muhammad Rizwan Ashraf |
| 110 ## - MAIN ENTRY--CORPORATE NAME |
|---|
| Location of meeting |
Mr. Muhammad Asif |
| 245 ## - TITLE STATEMENT |
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| Title |
Molecular Characterization Of Local Isolation Of Staphylococcus Aureus On The Bsis Of 16S Rrna From Poulry And Their Transmission to Humans |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Year of publication |
2012 |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Staphylococcus aureus is a widely distributed throughout the world and makes up the normal bacterial flora of skin and mucous membranes of man and animals. It is involved in suppurative wound infections in man and animals. Poultry industry has also been effected by S. aureus and causing great economic and health problems. The focus of the microbiology is to correctly identify S. aureus for the treatment of the animals. Molecular biology and biotechnology is proving a helping hand in the accurate identification of microorganisms through sequence analysis of 16S rRNA gene. The aim of this study was the molecular characterization of S. aureus from poultry and poultry farm workers through 16S rRNA analysis. Bacteria were collected from poultry and poultry farm human workers. All the samples were cultured and tested biochemically. In addition, peR amplification of 16S rRNA was performed in order to sequence the gene and further analyses through bioinforrnatics tools were performed. The aim of the study was the molecular characterization of S. aureus in poultry and humans through 16S rRNA sequencing, finding the phylogenetic relationships among S. aureus isolates and detection of zoonoses between poultry and human. 16S rRNA gene was amplified with peR primers and the sequence was compared with NeBI database reported S. aureus sequences. Resemblance was found between human and chicken isolates. Phylogenetic analyses were performed by using MEGA5 so ftware that also showed phylogenetic relationship among them.
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| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical Term |
Institute of Biochemistry & Biotechnology |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Dr. Aby Saeed |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
Mr. Tanveer Hussain |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Koha item type |
Thesis |
