Antibiotic Resistance Pattern Of Staphlococcus Aureus And Its Resistance Modulation Using Medicinal Plant Extracts (Record no. 8979)
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| 000 -LEADER | |
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| fixed length control field | 04090nam a22002057a 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20160815113510.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 160815b2016 xxu||||| |||| 00| 0 eng d |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| 082 ## - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 2501-T |
| 100 ## - MAIN ENTRY--AUTHOR NAME | |
| Personal name | Iqra Asif (2010-VA-279) |
| 110 ## - MAIN ENTRY--CORPORATE NAME | |
| Location of meeting | Prof. Dr. Aftab Ahmed Anjum |
| 245 ## - TITLE STATEMENT | |
| Title | Antibiotic Resistance Pattern Of Staphlococcus Aureus And Its Resistance Modulation Using Medicinal Plant Extracts |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Year of publication | 2016. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Number of Pages | 84p.; |
| 502 ## - DISSERTATION NOTE | |
| Dissertation note | This project was designed to evaluate the antimicrobial efficacy of Chloroform and ethanol extracts of Calotropis procera (C .procera) and Eucalyptus globulus (E. globulus) against Multiple Drug Resistant (MDR) Staphylococcus aureus isolated from human origin. This study was conducted to evaluate the antimicrobial potential of C. procera and E. globulus alone and in combination with antibiotics to check synergism between medicinal plants and resistant antibiotics. S.aureus is a major pathogen which causes various infections. Infectious diseases affect millions of people around the world and in the history these diseases are major cause of mortality and morbidity across the globe. In past few decades rate of mortalities are continuously increasing because of acquired resistance of S. aureus against multiple drugs, thus it is utter need of time to discover some alternatives to antibiotics so that we can resolve this dilemma of antibiotic resistance. Plant extracts are hope for this purpose as they have many compounds which have potential to lower down the number of micro-organisms. Plants have benefits over other as they are non toxic, non-reactive and have least side effects. Total 20 samples of human origins were procured from Department of Microbiology, UVAS Lahore and were subjected to check their antibiotic resistance profile against Erythromycin, Amoxicillin and Ciprofloxacin by Kirby Bauer disc diffusion assay. Out of 20, nine resistant isolates were separated. Among them three were resistant to Erythromycin, three to Amoxicillin and three to Ciprofloxacin. Summary 72 Leaves of C. procera and E. globulus were processed in Chloroform and ethanol Solvents. Antimicrobial activity was evaluated by agar gel well diffusion assay in which zone of inhibitions were measured. Minimum inhibitory concentration (MIC) of plant extracts was evaluated by micro broth dilution method. Best antimicrobial activity was observed by ethanolic extract of E. globulus. Then combine effect of sub-inhibitory concentrations (SICs) of plant extracts and minimum inhibitory concentration of antibiotics were determined by Well Diffusion assay. Four different sub inhibitory concentrations of plant extracts i.e.10μg/ml, 20μg/ml, 40μg/ml and 80μg/ml were used in combination with fixed concentration of antibiotics i.e.100μg/ml to check combinational effect of both. At selected sub-inhibitory concentration plant extract alone did not show any antibacterial activity. Two of the isolates had shown modulation when amoxicillin and plant extracts combination was used against them. The isolate labeled as S.aureus 4 showed modulation with the use of Ethanolic extract of Calotropis procera and S.aureus 5 had shown modulation with the use of chloroform extract of Calotropis procera. For further confirmation two more concentrations of 160μg/ml and 320μg/ml were used along with100μg/ml Amoxicillin against same isolates S.aureus 4 and 5. Zone of inhibition was observed with increased diameter indicating modulation of two isolates. While erythromycin and ciprofloxacin resistant isolates didn’t show any modulation. Data of antibiotic resistance and resistance modulation using plant extracts was analyzed by one way analysis of variance (ANOVA) followed by Duncan’s multiple range(DMR) posthoc test using Statistical package for social sciences (SPSS) 17.0 Statistical software at α < 0.05. |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical Term | Department of Microbiology |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Prof. Dr. Khushi Muhammad |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ms. Tehreem Hussain |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Thesis |
| Damaged status | Collection code | Permanent Location | Current Location | Shelving location | Date acquired | Full call number | Accession Number | Koha item type |
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| Veterinary Science | UVAS Library | UVAS Library | Thesis Section | 2016-08-15 | 2501-T | 2501-T | Thesis |