Efficient fertility of lactating dairy cows has always been the doorstep to economically profitable dairy farming. It is mostly agreed that uterine diseases in the cow after parturition have a negative effect on overall reproductive performance. Ozone which is assumed as a very potent oxidant, is one among these alternative techniques. The advantage of using ozone rather than antibiotics is in lowering the incidence of bacterial resistance in consumers of foodstuffs of animal origin, and other advantage is that ozone has no withdrawal period for milk, meat and other products. Ozone breaks through the microorganism (bacteria and germs) cell membrane, and also destroys viruses by diffusing through the protein coat in the nucleic acid core, resulting in damage of the viral nucleic acid.
The study was conducted on 50 animals which were randomly divided in two groups. Both groups were having 25, 25 animals. Group A received Ozone while group B received gentamicin sulphate intra uterine. Uterine lavage was taken twice, once before applying treatment and second after 8 hours of applying treatment. Samples were cultured for bacteriology to detect E.coli, F. necrophorum, A. pyogenes and St. pyogenes. Number of positive cases in Group A was 12, 9, 10 and 7 for E. coli, F. necrophorum, A. pyogenes and St. pyogenes respectively. Number of positive cases in Group B was 10, 8, 11 and 8 for E. coli, F. necrophorum, A. pyogenes and St. pyogenes respectively. After applying certain biochemical tests for each bacteria, bacteria was confirmed.
Difference of the colony forming units of before and after applying treatments for each bacterium in both groups was calculated. This difference was compared with difference of the colony forming units for same bacteria of other group by using Independent 2 samples T-test with the help of Statistical Packages for Social Sciences (SPSS Inc.) for the windows Version 13.3 (Chicago IL, USA).
Results were interpreted on the basis of level of significance. Differences among the groups were considered significant at P < 0.05. E. coli, F.necrophorum and St. pyogenes were highly significant as the P value for group differences was less than 0.05. Group differences among S.pyogenes showed no significance as the P>0.05.
The results showed that ozone is better in efficacy as compared to gentamicin sulphate on uterine infections in cows.