Biochemistry is gaining ground quickly due to the different preferences that it offers over customary chemical procedures particularly in regards to environment and cost contribution. Now a day’s industrial enzymes speak to the heart of biochemistry forms. Proteases are groups of organic catalysts. They have catalytic function.They hydrolyze peptide obligations of proteins and to separate them into polypeptides or free amino acids. Proteases are universal in nature and found in a wide range of differing qualities of sources, for example, plants, animals and microorganisms. Proteases do different organic procedures. Diverse types of proteases are available, alkaline proteases are a standout amongst the most imperative enzymes. The cost of the strain is a special in enzyme production and their applications in different industrial processes. The imaginative part of the present work was to distinguish and detach alkaline proteases from different organic sources, for example, from soil microorganisms with a reason to have beneficial outcome for hydrolyzing the proteins. The purified strain was checked for their potential part in chicken as a probiotics.
Protease producing bacteria act as probiotic to improve digestion which results in weight gain in broiler. In this research project, bacterial strains were isolated from decaying organic plants and soil samples collected from decaying organic plants of UVAS Lahore, Pakistan. Soil and decaying samples (1.0 g) were mixed in 100 ml normal saline, afterward serially diluted from 10−1 to 10−6 ratio with normal saline. 100 μl of each diluted sample inoculated in cultural medium and incubated at 37 °C for 48 h. The isolated colonies were selected to obtain pure bacterial cultures. Cultures were maintained in nutrient agar slants. Bacilli were isolated and screened from soil samples for production of proteases. The strains were identified morphologically, biochemically and on their molecular basis. The enzyme (protease) activity was assayed according to standard procedures by slight modifications. The strain was grown and fed to chicken as probiotic. The effects of probiotic were tested for growth of chicken. The probiotics improved the intestinal microbial balance and helped broiler in gaining weight. Bacterial stain studied in the current project may be used on commercial scale for promoting or enhancing the growth of broiler chicken.