A major nutritional problem these days is the biological and chemical contamination of food with environmental chemicals. Bisphenol-A (BPA) is one of a chemical which is produce worldwide and cause food contamination. BPA has many genomic and non-genomic effects and target various organs of both human and animals and cause hazardous effects. BPA cause other diseases such as angina, coronary heart disease, heart attack, stroke, asthma, emphysema, chronic bronchitis, arthritis, thyroid hormone disruption, liver damage and disrupted beta cell function. It has adverse effects on the homeostasis of glucose which leads to the metabolic disorders like insulin resistant type 2 diabetes. BPA also affect the barrier function of small intestine. The current study was aimed to investigate the effect of BPA on the barrier function of intestine.
Bisphenol-A (BPA) wasdisrupt glucose uptake and perturb the gut barrier function. Our study aimed to find out the effect of Bisphenol-A on the electrophysiology of ileum of small intestine of rabbit.For this purpose twenty healthy rabbits of uniform age and weight were selected and slaughtered at slaughter house of UVAS. Ileum was taken from the intestinal part and rinsed with standard buffer. Serosallayer was removed and tissue was mounted in Ussing Chamber. After incubation period of 15-20mins different groups were treated with different concentrations of BPA anddivided into four groups (A, B, C, and D), each having five rabbits. Group A, was control. In group B, tissue was treated with Bisphenol-A 5 µM. Group C was treated with 15 µM of Bisphenol-A, and group D was treated with 30µM of Bisphenol-A. After treatment the electrophysiological parameters like short circuit current (Isc), conductance (Gt) and potential difference (mV)was observed. BPA affected the electrophysiology of isolated ileum of rabbit by decreasing the short circuit current. The decrease of current between the groups is significant (p value < 0.05). Iscwas significantly decrease between the groups containing 5µМ BPA and control group.Gtwas significantly different from group 5µМ and group 15µМ of BPA.mV was significantly different from control group and group 15µМ of BPA.
Statistical analysis was conducted with statistical packages for social sciences (SPSS). The data were analyzed using one way analysis of variance (ANOVA). Post hoc test was carried out using Tukey. P value was considered significant at P<0.05. The result of this study showed that BPA 5µM effects the electrophysiology of intestine as compared to other groups (15 µM and 30 µM). Suggesting a possible role of BPA in affected intestinal barrier function and glucose uptake.