In-Vitro Effect Of Chromium Chloride On The Electrophysiological Indices Of Jejunum In Broilers.
By: Maria Khalid Majeed (2014-VA-961)
| Prof. Dr. Habib ur Rehman.
Contributor(s): Dr. Sajid Khan Tahir | Dr. Wasim Shehzad.
Material type: 
BookPublisher: 2017Description: 33p.Subject(s): PhysiologyDDC classification: 2869-T Dissertation note:
Chromium is a well-known component of glucose tolerance factor, which participates in glucose metabolism by enhancing the effects of insulin. The Ussing chamber is a very powerful technique to study ion transport across tissues. This study is to determine the effect of chromium chloride hexahydrate on electrophysiology of jejunum and its interaction with glucose transport.
According the background we concluded the hypothesis i-e Chromium chloride may enhance the glucose transport in jejunum mucosa of the broilers. To approach this hypothesis, we performed an experiment into different groups. In overall experiment, we take Twelve broilers of 35 days of age was procured from local farm. After a week, birds were slaughtered; jejunum section of the birds were isolated then stripped off serosal layer. Each segment of the intestine was mounted and labelled on ussing chamber separately with groups viz, Group1 (0μM, control), Group2 (5μM CrCl3), Group3 (10μM CrCl3). After baseline, add CrCl3 to the mucosal side of UC and note the reaction. Then Add glucose after incubation period. The peak response obtained within 2-3 mins. Then the change in Isc (ΔIsc) was calculated approximately after addition of CrCl3 and glucose. The PD, Isc, and Gt were measured by voltage/current clamp. The data was analysed using one-way ANOVA and was presented as mean±SEM. Group differences were compared by Duncan multiple range Test (P<0.05). The current study showed that there are no significant differences shown in initial short circuit current and ▲Isc and tissue conductance before and after adding the chemicals. In conclusion, it is hoped that results presented in the current research would open avenues for further discussion and more concerted investigations into each of the area covered. More specifically, there are no previous research reports on the in vitro effect of chromium chloride hexahydrate in poultry.
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 2869-T (Browse shelf) | Available | 2869-T |
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Chromium is a well-known component of glucose tolerance factor, which participates in glucose metabolism by enhancing the effects of insulin. The Ussing chamber is a very powerful technique to study ion transport across tissues. This study is to determine the effect of chromium chloride hexahydrate on electrophysiology of jejunum and its interaction with glucose transport.
According the background we concluded the hypothesis i-e Chromium chloride may enhance the glucose transport in jejunum mucosa of the broilers. To approach this hypothesis, we performed an experiment into different groups. In overall experiment, we take Twelve broilers of 35 days of age was procured from local farm. After a week, birds were slaughtered; jejunum section of the birds were isolated then stripped off serosal layer. Each segment of the intestine was mounted and labelled on ussing chamber separately with groups viz, Group1 (0μM, control), Group2 (5μM CrCl3), Group3 (10μM CrCl3). After baseline, add CrCl3 to the mucosal side of UC and note the reaction. Then Add glucose after incubation period. The peak response obtained within 2-3 mins. Then the change in Isc (ΔIsc) was calculated approximately after addition of CrCl3 and glucose. The PD, Isc, and Gt were measured by voltage/current clamp. The data was analysed using one-way ANOVA and was presented as mean±SEM. Group differences were compared by Duncan multiple range Test (P<0.05). The current study showed that there are no significant differences shown in initial short circuit current and ▲Isc and tissue conductance before and after adding the chemicals. In conclusion, it is hoped that results presented in the current research would open avenues for further discussion and more concerted investigations into each of the area covered. More specifically, there are no previous research reports on the in vitro effect of chromium chloride hexahydrate in poultry.
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