This study was designed to evaluate the beneficial effects of montelukast against anti- cancerous drug methotrxate and to evaluate the hepatoprotective effect of montelukast on blood serum in rats and to find the hepatoprotective effecst on liver after methotrexate induced toxicity in rats. Methotrexate (MTX) is used as anti-cancerous drugs used in different malignancies and immunological issues like hepatotoxicity and bone marrow depletion
Montelukast is an anti-asthmatic drug, which act as antagonist of cysteinly leukotriene
receptors,in inflammation and oxidative stress. Montelukast decrease the risk of hepatic damage after cancerous drug treatment decrease the liver enzymes by inhibiting the cysteinyl leukotriene receptors.
In University Of Veterinary And Animal Sciences, thirty Wister rats, six rats in each five group were kept in stainless steel cages and following treatment plan was given to animals.
Group 1: (Negative control) injected I/P with physiological saline from day zero to day four and then given with 2% ethanol from day four to day ten.
Group:2 (MK positive control) injected I/P with MK(10mg/kg body weight, BW) from day four to day ten for consecutive seven days.
Group 3: (MTX positive control) injected at day zero I/p with a singl dose of MTX (20mg/kg BW) per ten days.
Group 4: (MTX-MK 5) were given at day zero I/P with a single dose of MTX (20mg/kg BW) and then injected I/P with MK (5mg/kg BW) from day four to day ten for consecutive seven days.
Group 5: (MTK-MK 10) were given at day zero I/p with a single dose of MTX (20mg/kg BW) and then given I/P with MK (10mg/kg BW) from day four to day ten for consective seven days.
One way analysis of variance (ANOVA) is used for data analysis. Social sciences (SPSS) with the statistical package analysis was conducted inc. window version 20, Chicago, IIinois). Mean ± S.E.M was used for data presented. Duncan’s Multiple range test differences used for the group differences comparison.
Results
Montelukast decrease the risk of hepatic damage after cancerous drug treatment decrease the liver enzymes montelukast used against anti- cancerous drug methotrxate, and to evaluate the hepatoprotective effect of montelukast on blood serum in rats to find the hepatoprotective effects on liver after methotrexate induced toxicity in rats.
Conclusion
End of this study we have concluded that methotrexate toxicity can be normalize with the use of montelukast, it reduce the chances of hepatic damage and serum cholesterol, high density lipids and triglycerides. Montelukast significantly reduce the total cholesterol, ALT and bilirubin level. it also normalize the bilirubin and albumin level in serum.