Passive Immuniation Of Newcastle Disease Virus Infected Birds
By: Raheel Arshad
| Dr . Khushi Muhammad.
Contributor(s): Dr . Khalid | Dr . Sa meera Akhtar | Faculty of Veterinary Sciences.
Material type: 
BookPublisher: 2000Subject(s): Department of MicrobiologyDDC classification: 0679,T Dissertation note: Present study was designed to determine the contribution of maternal immunity against the Newcastle disease (ND). In the study two hundred and ten white leghorn cockerels were used. A velogenic ND virus field isolate was used for the challenge. Lethal Dose 50 (LD5O) of the velogenic strain of ND virus was calculated which was 10.727. Maternal antibody levels of the chicks were determined on weekly basis by using the haemaggldtination inhibition (HI) test. The maternal geometric mean HI titre recorded at 1, 7, 14, 21 and 28 days of the age of birds were 147, 157.6, 22.6, 13 and 2.6 respectively. The protection offered by maternal immunity in chicks challenged with 100 LD50 at 1, 7, 14, 21 and 28 days of age was, 100, 100, 70, 10 and 0 percent respectively. It was concluded that birds having maternal hurnoral immunity more than 64 GMT showed 100% protection to challenge infection. The effect of immunized egg yolk to ND infected birds was also studied. The HI titre of immunized yolk was determined and then different HI units of the immunized yolk were prepared with normal saline. It was observed that egg yolk (1 ml, 64 HI units) injected to ND challenged birds showed 100% protection as compared to that of control group (given 1 ml of 0 HI units of the yolk). The cost of the production of immunized yolk was also determined that was Rs.0.35 / dose (1 ml : 64 HI units).
From the study, it was inferred that the hyper immunized yolk can be used as therapeutic agent to cure the ND infected birds.
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 0679,T (Browse shelf) | Available | 0679,T |
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Present study was designed to determine the contribution of maternal immunity against the Newcastle disease (ND). In the study two hundred and ten white leghorn cockerels were used. A velogenic ND virus field isolate was used for the challenge. Lethal Dose 50 (LD5O) of the velogenic strain of ND virus was calculated which was 10.727. Maternal antibody levels of the chicks were determined on weekly basis by using the haemaggldtination inhibition (HI) test. The maternal geometric mean HI titre recorded at 1, 7, 14, 21 and 28 days of the age of birds were 147, 157.6, 22.6, 13 and 2.6 respectively. The protection offered by maternal immunity in chicks challenged with 100 LD50 at 1, 7, 14, 21 and 28 days of age was, 100, 100, 70, 10 and 0 percent respectively. It was concluded that birds having maternal hurnoral immunity more than 64 GMT showed 100% protection to challenge infection. The effect of immunized egg yolk to ND infected birds was also studied. The HI titre of immunized yolk was determined and then different HI units of the immunized yolk were prepared with normal saline. It was observed that egg yolk (1 ml, 64 HI units) injected to ND challenged birds showed 100% protection as compared to that of control group (given 1 ml of 0 HI units of the yolk). The cost of the production of immunized yolk was also determined that was Rs.0.35 / dose (1 ml : 64 HI units).
From the study, it was inferred that the hyper immunized yolk can be used as therapeutic agent to cure the ND infected birds.
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