Immuno Prophylaxis Of Entrerotoxaemia In Sheep And Goats
By: Shahzad Jawed
| Dr . Muhammad Naeem.
Contributor(s): Dr . Asif | Dr . Khushi Muhammad | Faculty of Veterinary Sciences.
Material type: 
BookPublisher: 1999Subject(s): Department of MicrobiologyDDC classification: 0683,T Dissertation note: The present project was designed to study the comparative efficacy of three different adjuvant (Potassium aluminium sulphate, Aluminium hydroxide gel and mineral oil) for enhancing the immune potential of enterotoxaemia (combined) vaccine. T)ifferent standard biological media alongwith the supplementation of amino acids, minerals, plants and animal extracts and special modified formulated media were used for the production of alam precipitated vaccine, aluminium hydroxide gel adsorbed vaccine and oil adjuvant vaccine.
It was concluded that addition of various ingredients, including yeast extracts, trace elements, amino acids, plants and animal extract, and cystine hydrochloride in proper concentration, increased the level of prototoxin and toxin in culture media due to the availability of essential required nutrients. Mouse model was chosen to study the safety and potency test of all the vaccines. The potency of all three vaccines was compared. In this experiment alam precipitated vaccine proved inferior to aluminium hydroxide gel adsorbed and oil adjuvant vaccine. In case of oil based vaccine especially in sheep the IHA antibody was significant in vaccine having potency of 750 HU/ml but on the other hand vaccine have potency 250 and 500 HU/ml were proved non significant, and the day 45 was proved significant in developing the antibody titre than that of 15 and 30 days. Same the picture was observed in case of goals that oil adjuvant vaccine was significant that of aluminized and toxoid adsorbed vaccine. It was observed that the protection afforded to goats by multivalent clostridial vaccine was higher than afforded to sheep.
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 0683,T (Browse shelf) | Available | 0683,T |
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The present project was designed to study the comparative efficacy of three different adjuvant (Potassium aluminium sulphate, Aluminium hydroxide gel and mineral oil) for enhancing the immune potential of enterotoxaemia (combined) vaccine. T)ifferent standard biological media alongwith the supplementation of amino acids, minerals, plants and animal extracts and special modified formulated media were used for the production of alam precipitated vaccine, aluminium hydroxide gel adsorbed vaccine and oil adjuvant vaccine.
It was concluded that addition of various ingredients, including yeast extracts, trace elements, amino acids, plants and animal extract, and cystine hydrochloride in proper concentration, increased the level of prototoxin and toxin in culture media due to the availability of essential required nutrients. Mouse model was chosen to study the safety and potency test of all the vaccines. The potency of all three vaccines was compared. In this experiment alam precipitated vaccine proved inferior to aluminium hydroxide gel adsorbed and oil adjuvant vaccine. In case of oil based vaccine especially in sheep the IHA antibody was significant in vaccine having potency of 750 HU/ml but on the other hand vaccine have potency 250 and 500 HU/ml were proved non significant, and the day 45 was proved significant in developing the antibody titre than that of 15 and 30 days. Same the picture was observed in case of goals that oil adjuvant vaccine was significant that of aluminized and toxoid adsorbed vaccine. It was observed that the protection afforded to goats by multivalent clostridial vaccine was higher than afforded to sheep.
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