The mature breeding buffalo bull at the Semen Production Unit has greater importance to produce maximum doses and higher fertility rate in the field. The sire should not only be of high genetic make-up but should also be in good sexual health for the production of large number of fertile germ cells based on seminal characteristics.

The present project was designed to investigate the production potential and reproductive performance (fertility trials) of the donor buffalo bulls. On the basis of available data the investigations were carried out in two groups comprising of standard bulls and routine donors. The standard bulls were offered mixed bull ration while routine donors were kept on routine management and available fodder regime.

It is evident from the results that standard buffalo bulls had better and encouraging performance. The mean values of all the seminal parameters among different age groups were comparatively higher .in the bulls fed on additional mixed bull ration than the group of bulls kept on green fodder and routine maintenance ration only.

Although superior seminal values were obtained but statistically the differences within age groups were found to be nonsignificant for both feeding regimes. It may be due to limited duration. However, within various seminal characteristics highly significant differences were observed. After evaluating the semen in the laboratory it is of utmost importance to test this semen in the field conditions because the ultimate determinant of the semen fertility is the conception rate. A total of 3807 doses of semen from ten bulls were distributed in 10 selected A.I. centers. The first insemination resulted in an average fertility rate of 56.52±3.97% whereas the fertility rate of second insemination was 60.37%. The average fertility resulted from all bulls were 56.52±3.97%. Among the bulls the fertility rates were non significantly different. Similarly, mean fertility rates from each A.I. center were not significantly different. It was also observed that there was no significant difference in the fertility rates among various months of semen collection and insemination. The fertility results are better in aged buffaloes when compared to those of heifers (57.99 Vs 54.74%).

Similarly, to compare the effect of three diets, having crude proteins and digestible nutrients from different sources on seminal attributes had indicated better results for implementation at the Semen Production Unit. Seasonality month of the year affected all the seminal attributes. Values of the winter months (November to January) were better than the other three seasons. October was the best month, while the June had worst effect on the semen quality.