Charecterization Of Peste Des Petits Ruminants Virus (Local Strain) From Small Ruminants
By: Sher Bahadar Khan
| Dr.Aftab Ahmad Anjum.
Contributor(s): Dr.Azhar | Dr.Irshad Hussain | Faculty of Veterinary Sciences.
Material type: 
BookPublisher: 2008Subject(s): Department of MicrobiologyDDC classification: 1039,T Dissertation note: The objectives of the study were to isolate PPR virus (Local strain) using Vero cell line, Identification of PPRV through Cytopathic effects (CPE) produced by PPRV on Vero cell line. To detect haemeagglutinability of PPR virus with RBCs of poultry, duck, goat, pigeon, sheep, horse and human through Haemeagglutination test. And confirmation of PPR virus through Hemeagglutination inhibition test and Immunocapture Enzyme Linked Immunosorbent Assay (Ic ELIZA). For this purpose 120 tissue samples (40 Necrotic debris in buccal mucosa, 30 each nasal and ocular discharges and 20 lymph nodes) were collected from clinical positive cases. These samples were moistened with 2-3 drops sterile PBS and were brought immediately to the laboratory in sterilized universal container under refrigeration temperature. The tissue samples were processed for virus isolation. After sterilization of the glassware, Dehydrated modified Essential Medium (DMEM) was prepared according to the manufacturer's instructions for cultivation of Vero cells. Stock solution of phenol red, Carbonate/bicarbonate buffer, Stock antibiotic solution, Trypsin, Versene solution and Trypsin-versene (TV) solution were prepared. The inoculums of Pestes des Petitis ruminants virus was filtered through 0.2um pore sized syringe filter (Millipore,USA) and transferred to a pre-sterilized McCartney bottle.When a complete monolayer of the Vero cell line was formed and almost 80 % confluency was obtained, the exhausted medium from the carrel flask was discarded and new filtered and sterile maintenance medium (25 ml) was added per flask. The virus inoculum was inoculated on Vero cell line and examined daily for CPE. The haemeagglutinability of the virus was checked with RBCs of chicken, duck, pigeon, sheep, goat, horse and human blood group 0. The The haemeagglutinability of the virus was also checked under different conditions i.e. influence of diluents, influence of temperature and influence of incubation. Finally Haemeagglutination inhibition test and ic ELISA was performed.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|---|
Thesis
|
UVAS Library Thesis Section | Veterinary Science | 1039,T (Browse shelf) | Available | 1039,T |
The objectives of the study were to isolate PPR virus (Local strain) using Vero cell line, Identification of PPRV through Cytopathic effects (CPE) produced by PPRV on Vero cell line. To detect haemeagglutinability of PPR virus with RBCs of poultry, duck, goat, pigeon, sheep, horse and human through Haemeagglutination test. And confirmation of PPR virus through Hemeagglutination inhibition test and Immunocapture Enzyme Linked Immunosorbent Assay (Ic ELIZA). For this purpose 120 tissue samples (40 Necrotic debris in buccal mucosa, 30 each nasal and ocular discharges and 20 lymph nodes) were collected from clinical positive cases. These samples were moistened with 2-3 drops sterile PBS and were brought immediately to the laboratory in sterilized universal container under refrigeration temperature. The tissue samples were processed for virus isolation. After sterilization of the glassware, Dehydrated modified Essential Medium (DMEM) was prepared according to the manufacturer's instructions for cultivation of Vero cells. Stock solution of phenol red, Carbonate/bicarbonate buffer, Stock antibiotic solution, Trypsin, Versene solution and Trypsin-versene (TV) solution were prepared. The inoculums of Pestes des Petitis ruminants virus was filtered through 0.2um pore sized syringe filter (Millipore,USA) and transferred to a pre-sterilized McCartney bottle.When a complete monolayer of the Vero cell line was formed and almost 80 % confluency was obtained, the exhausted medium from the carrel flask was discarded and new filtered and sterile maintenance medium (25 ml) was added per flask. The virus inoculum was inoculated on Vero cell line and examined daily for CPE. The haemeagglutinability of the virus was checked with RBCs of chicken, duck, pigeon, sheep, goat, horse and human blood group 0. The The haemeagglutinability of the virus was also checked under different conditions i.e. influence of diluents, influence of temperature and influence of incubation. Finally Haemeagglutination inhibition test and ic ELISA was performed.
There are no comments for this item.