Pathology Of Naturally Infected Broilers With Mycoplasma Gallisepticum And Its Diagnosis Through Pcr
By: Aamir Islam
| Dr.Asim Aslam.
Contributor(s): Prof.Dr.Mansur- | Prof.Dr.Zafar Iqbal Ch | Faculty of Veterinary Sciences.
Material type: 
BookPublisher: 2009Subject(s): Department of PathologyDDC classification: 1055,T Dissertation note: Two hundred broiler birds (200) showing the clinical signs of respiratory signs from fifty (50) poultry farms located in and around Lahore District were analyzed for the detection of Mycoplasma gallisepticum. The tissue samples (trachea and lungs) were subjected to PCR using for Mycoplasma gallisepticum 16S rRNA gene amplification with a set of primers (MG14-F and MG13-R). Out of 200 samples, 86 were found positive with MG. These positive samples were further analyzed for histopathological changes. Lungs showed hemorrhages, congestion and massive necrosis. Lymphocytic infiltration and oedema was also observed in lungs sections. Liver showed coagulative necrosis around the central vein, congestion and infiltration of lymphocytes. Similarly, heart section revealed necrosis and degeneration in cardiac muscles. Trachea revealed the epithelial and mucosal infiltration with lymphocytes. Hypertrophy of epithelial mucosa and catarrhal exudates recorded in trachea. Sloughing of the mucosa and sub mucosa of varying degree was noted in trachea. Few birds showed no obvious changes in the organs but were positive on PCR analysis.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode | Item holds |
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 1055,T (Browse shelf) | Available | 1055,T |
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Two hundred broiler birds (200) showing the clinical signs of respiratory signs from fifty (50) poultry farms located in and around Lahore District were analyzed for the detection of Mycoplasma gallisepticum. The tissue samples (trachea and lungs) were subjected to PCR using for Mycoplasma gallisepticum 16S rRNA gene amplification with a set of primers (MG14-F and MG13-R). Out of 200 samples, 86 were found positive with MG. These positive samples were further analyzed for histopathological changes. Lungs showed hemorrhages, congestion and massive necrosis. Lymphocytic infiltration and oedema was also observed in lungs sections. Liver showed coagulative necrosis around the central vein, congestion and infiltration of lymphocytes. Similarly, heart section revealed necrosis and degeneration in cardiac muscles. Trachea revealed the epithelial and mucosal infiltration with lymphocytes. Hypertrophy of epithelial mucosa and catarrhal exudates recorded in trachea. Sloughing of the mucosa and sub mucosa of varying degree was noted in trachea. Few birds showed no obvious changes in the organs but were positive on PCR analysis.
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