Feeding management experiment was conducted at Buffalo Research Institute, Pattoki on thirty nine lactating Nih Ravi buffaloes divided into three groups thirteen in each kept under tie stall intensive management for a period of four weeks. All the three groups were given roughages ad libitum and concentrate according to their milk production level. The milk production was recorded daily in the morning and evening. Milk samples were collected weekly by mixing the milk produced in the morning and evening and analyzed for various milk composition parameters. The blood from jugular veins of the selected buffaloes was also collected on weekly basis and analyzed for blood bio-chemistry in the WTO quality control laboratory.

Highest milk production per day (9.78+O.O9ml) was recorded in T2, followed by TI and T3. Statistically analyzed data showed highly significant (PO.05) differences between treatments. The milk analysis of buffaloes kept Ofl treatments TI, T2 and T3 showed milk fat contents 5.58±0.08, 5.70f0.08 and 6.031-0.08% respectively. SNF% in buffaloes was 8.55±0.16, 8.41+0.16 and 8.20±0.16 respectively. Statistically analyzed data showed significant (P<0.05) differences in fat and non significant (P>0.05) differences in SNF contents between treatments. Feed samples were collected for proximate analysis at laboratory of Animal Nutrition Department UVAS and results revealed that concentration was compared of 17.41, 18.26 and 71 % Dry matter, Crude protein, and TDN, respectively. Blood sample were analyzed for total protein, triglyceride, urea and blood glucose. The blood glucose contents value were 3.96±0.58, 4.08±0.60 and 4.72+ 0.63 mg/uI in buffaloes on treatments Ti, T2 and T3 respectively. Total protein values for each treatment were 6.37±0.54, 6.74±0.55 and 5.97±0.58 mg/dl. Triglycerides were 5.58±0.58, 4.16±0.59 and 4.33+0.62 mg/dl in buffaloes on treatment 1. 2 and 3 respectively. Mean Urea level was 1.40-f 0.024. 1.03+0.25 and 1.56+0.26 mg/dl.
The following conclusions have been drawn.
- Methionine supplementation increases the overall milk production in buffaloes.

- Methionine supplementation has variable effect on different milk

- composition parameters. It increases the milk fat percentage but has least effect on other parameters.

- Methionine supplementation increases the blood glucose and has no effects on other blood parameters.