The study was conducted to understand the prevalence of subclinical mastitis prevalence of non-functional teats and various factors associated with it, viz lactation age, lactation number, housing and hygiene. The economical losses due to subclinical mastitis and non-functional teats were also estimated. Four village Munianwala, Kot Lehnadas, Rossy and Damiranwala and two private farms located in Jattan Tha Warda and Wazirpur were selected on the basis of availability and non-availability of veterinary medical services.
The prevalence of subclinical mastitis was found to be 39.46% (58 affected cattle out of 147 lactating cattle) in cattle and 29.13% (162 affected buffaloes out of 556 lactating buffaloes) in buffaloes on animal basis and 12.79% (37 affected quarters out of 2791 quarters at risk) on quarter basis collectively in both species.

The occurrence of subclinical mastitis was higher in hindquarters 60% than 40% in fore quarters. The prevalence of subclinical mastitis was recorded, 32.21%, 27.73%, 21.84% and 18.20% in right hind, left hind, left front and right front quarters respectively from 220 affected animals.

The prevalence of subclinical mastitis was highest in 5th and 6th lactation animals, 36.23% in buffaloes and 42.85% in cattle followed by 3rd and 4th lactation animals 32.57% in buffaloes and 39.39% in cattle and 1st and 2nd lactation animals 24.50% in buffaloes and 39.34% in cattle. The lowest prevalence of subclinical mastitis was in group of animals which were in > 6th lactation.

The highest prevalence of subclinical mastitis was observed in late stage of lactation in both buffaloes and cattle i.e. 33.65% and 56.09% respectively. The prevalence of subclinical mastitis was found 27.71% in buffaloes and 31.57% in cattle in mid stage of lactation and 26.11% in buffaloes and 34.66% in cattle in early stage of lactation.

The prevalence of subclinical mastitis in animals with poor, fair and good hygienic status was found to be 50%, 30.58% and 19.62% respectively.

The prevalence of non-functional teats was found to be 0.75% (21 non-functional teats out of 2812 quarters at risk) on quarter basis and 2.84% animals (n=20/703) had non-functional teats.

The economical losses due to non-functional were estimated to be Rs.340 per lactating animal and Rs.929 per farmer per annum. Losses due to subclinical mastitis were estimated to be Rs.536 per lactating animal and Rs.1467 per farmer per annum.