Angiogenesis, the growth of new capillary blood vessels in the body, has much more importance in healing and reproduction. The body controls angiogenesis as there is a natural balance between growth and inhibitory factors in healthy tissues.
When this balance is disturbed, the result is either too much or too little angiogenesis. Abnormal blood vessel growth, either excessive or insufficient, is now recognized as a "common denominator" underlying many deadly and debilitating conditions, including cancer, skin diseases, age-related blindness, diabetic ulcers, cardiovascular disease, stroke, and many others.
Treating angiogenesis with anti-angiogenic compounds results in safe and effective anti-angiogenic therapies. In current experiment, focus was on the effect of Diclofenac sodium on angiogenesis using chicken chorio-allantoic membrane (CAM) assay. Fresh fertilized eggs were taken, sprayed with 70% ethanol and incubated at 37 °C (humidity 55-60%). At day 5 of incubation a small window was made on each egg, 4-5 ml of albumin was aspirated, windows were sealed with sterile Para-film and eggs were returned to incubator. Concentrations i.e. 0.7%, 0.5%, 0.3% were prepared in distilled water. Filtered concentrations of the Diclofenac sodium were used for experimental analysis and applied to the CAMs on day 7 of incubation. Macroscopic vascular changes were evidently observed among all treated CAMs on day 6 of incubation. There was reduction in total area and diameter of primary, secondary and tertiary blood vessels was observed after treating CAMs with Diclofenac sodium. By using SPIP (Scanning probe image processor) software, 3D surface roughness measurements, Sa (surface area ), Sq (root mean square), Ssk (surface deviation), Sku (peakedness), Sdr (ratio of increment of interfacial area of a surface over sampling area), Sci (ratio of void volume of the unit sampling area at core zone over root mean square deviation ), Sy (lowest valley), Sz (average absolute height), Ssc (arithmetic mean submit), Sdq (root mean square sloop), Spk (reduce summit height), Sku (kurtosis of surface) , Stdi (texture index), Sk (core roughness depth) were carried out which clearly elaborated anti-angiogenic effect of Diclofenac sodium on CAMs. Diclofenac sodium inhibits angiogenesis, which is a common denominator shared by various major disease.