Dynamics Of Recombinant Avian Influenza Virus H9-Ha Gene Herpesvirus Of Turkey Vector Vaccine In Chicken
By: Shumaila Rani
| Prof.Dr.Masood Rabbani.
Contributor(s): Dr.Tahir Yaqub | Prof.Dr.Masroor Elahi Babar.
Material type: 
BookPublisher: 2010Subject(s): Department of MicrobiologyDDC classification: 1276,T Dissertation note: Avian influenza (AI) is known to exist for centuries. It's primarily a disease of birds. For prevention and control of H9N2 avian influenza disease, HA gene from Pakistani H9N2 field virus isolate (PK-UDL/01/08 H9N2) cloned into a herpesvirus of turkey (rHVT/AI-H9 having full HA) at Institute for Animal Health, Compton, UK, expressing HA proteins, was given subcutaneously (in the neck). Commercial avian influenza vaccine was given to chicken through intramuscular route.
On termination of the experiment, the chicks from all groups were sacrificed and their visceral organs were collected on chick-wise basis. Besides this, blood was collected for complete blood counts (CBC) and blood chemistry. Results of groups were then compared for any significant difference. The data analyses showed that in complete blood counts, there was no significant difference (p<0.05) of total leukocyte counts of different groups of chickens but heterophil percentages showed variation among different groups.
Analyses of Serum chemistry results showed that glucose and protein concentration in serum varies significantly (p>0.05) among the different groups of birds. However, there was no significant difference of cholesterol levels among the groups of chickens.
For determining the persistence of rHVT/AI-H9 having full HA virus in different visceral organ and in blood samples of Group A, results of the PCR showed the persistence of herpes turkey virus in leukocytes, spleen, liver, lung, kidney and heart. This project helped in evaluating dynamics of recombinant HVT containing avian influenza HA gene from avian influenza H9N2 Pakistani isolate.
In future the study may be continued for further biological characterization by isolating the HVT from different visceral organ in different interval of time and fractionating the serum protein and analyzing the gamma globulin fraction in serum to measuring the humoral immune response against the recombinant vaccine.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode | Item holds |
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 1276,T (Browse shelf) | Available | 1276,T |
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Avian influenza (AI) is known to exist for centuries. It's primarily a disease of birds. For prevention and control of H9N2 avian influenza disease, HA gene from Pakistani H9N2 field virus isolate (PK-UDL/01/08 H9N2) cloned into a herpesvirus of turkey (rHVT/AI-H9 having full HA) at Institute for Animal Health, Compton, UK, expressing HA proteins, was given subcutaneously (in the neck). Commercial avian influenza vaccine was given to chicken through intramuscular route.
On termination of the experiment, the chicks from all groups were sacrificed and their visceral organs were collected on chick-wise basis. Besides this, blood was collected for complete blood counts (CBC) and blood chemistry. Results of groups were then compared for any significant difference. The data analyses showed that in complete blood counts, there was no significant difference (p<0.05) of total leukocyte counts of different groups of chickens but heterophil percentages showed variation among different groups.
Analyses of Serum chemistry results showed that glucose and protein concentration in serum varies significantly (p>0.05) among the different groups of birds. However, there was no significant difference of cholesterol levels among the groups of chickens.
For determining the persistence of rHVT/AI-H9 having full HA virus in different visceral organ and in blood samples of Group A, results of the PCR showed the persistence of herpes turkey virus in leukocytes, spleen, liver, lung, kidney and heart. This project helped in evaluating dynamics of recombinant HVT containing avian influenza HA gene from avian influenza H9N2 Pakistani isolate.
In future the study may be continued for further biological characterization by isolating the HVT from different visceral organ in different interval of time and fractionating the serum protein and analyzing the gamma globulin fraction in serum to measuring the humoral immune response against the recombinant vaccine.
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