Molecular Characterisation And Antimicrobial Resistance Of Human Salmonella Enterica
By: Yaser Bhatti
| Dr. Atif Hanif.
Contributor(s): Prof. Dr.Masroor Elahi Babar.
Material type: 
BookPublisher: 2012Subject(s): Institute of Biochemistry & BiotechnologyDDC classification: 1382,T Dissertation note: Infectious diseases directly or indirectly are still the main cause of morbidity and mortality in the human population. Prevention and control of such diseases has been a major challenge since ages. During the last few decades, infections with Salmonella have been recognized as a major hazard to humans in most countries.In this study, ten confirmed and purifiedisolates ofSalmonella typhi were collected from Chugtai's Lahore Lab.and were grown inbuffered peptone water, tetrathionate broth and SS agar. Isolates were characterized by antibiotic resistance patterns, plasmid profiling and protein profiling. All isolates were resistant to ampicillin and were sensitive to ciprofloxacin and levofloxacin. 90% of isolates were sensitive to gentamicin and 50% were resistant to chloramhenicol, while 70% of isolates showed intermediate behaviour to amoxicillin. Single plasmid profile was observed among all resistant isolates and all isolates harboured a single heavy weight plasmid.Sensitive isolates were free of any plasmid. Isolates were grouped into six groups by SDS-PAGE. Different banding pattern was observed among groups. However, a protein of 10kDa was common in all isolates.
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 1382,T (Browse shelf) | Available | 1382,T |
Infectious diseases directly or indirectly are still the main cause of morbidity and mortality in the human population. Prevention and control of such diseases has been a major challenge since ages. During the last few decades, infections with Salmonella have been recognized as a major hazard to humans in most countries.In this study, ten confirmed and purifiedisolates ofSalmonella typhi were collected from Chugtai's Lahore Lab.and were grown inbuffered peptone water, tetrathionate broth and SS agar. Isolates were characterized by antibiotic resistance patterns, plasmid profiling and protein profiling. All isolates were resistant to ampicillin and were sensitive to ciprofloxacin and levofloxacin. 90% of isolates were sensitive to gentamicin and 50% were resistant to chloramhenicol, while 70% of isolates showed intermediate behaviour to amoxicillin. Single plasmid profile was observed among all resistant isolates and all isolates harboured a single heavy weight plasmid.Sensitive isolates were free of any plasmid. Isolates were grouped into six groups by SDS-PAGE. Different banding pattern was observed among groups. However, a protein of 10kDa was common in all isolates.
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