Assessment Of Knowledge And Practice Of Food Handlers About Food Safety In University Hostel Kitchen Employees
By: Qurra-Tul- Ain
| Prof. Dr. Mansur-ud-Din Ahmad.
Contributor(s): Prof. Dr. Muhammad Athar Khan.
Material type: 
BookPublisher: 2011Subject(s): Department of Epidemiology & Public HealthDDC classification: 1424,T Dissertation note: Cigarette butts are found at crime scenes as potential evidence. Saliva is present on the cigarette butts that can be detected and DNA can be isolated from nucleated cells present in saliva for quantification and typing. In past, research has declared that cigarette butts are useful source for saliva detection, DNA extraction and profile generation. Difference of saliva accumulation on cigarette butt paper and filter was compared in the present study. On smoked cigarette butts, gender based comparison of saliva detection and DNA quantification was performed.
Agarose gel assay was used for amylase enzyme detection. All samples showed positive results for saliva detection. Cigarette butt filter and filter paper were processed separately and analyzed. Results showed more saliva detected at cigarette butt paper. Then gender based comparison on cigarette butt samples smoked by males and females was done for salivary amylase. By using student t-test, no significant difference was found on basis of gender. For extraction of DNA, phenol chloroform extraction method was used. Quantifiler® Human DNA Quantification kit was used through RT-PCR for quantification of DNA isolated from cigarette butt papers. SDS software analyzed the data and gave results of quantified DNA in ng/µL. Gender based comparison in DNA quantity was done by using statistical method. SPSS software was used for statistical analysis. Pearson correlation value was calculated between detected saliva and quantified DNA of samples. Weak positive results of correlation were obtained between saliva and DNA.
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 1424,T (Browse shelf) | Available | 1424,T |
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Cigarette butts are found at crime scenes as potential evidence. Saliva is present on the cigarette butts that can be detected and DNA can be isolated from nucleated cells present in saliva for quantification and typing. In past, research has declared that cigarette butts are useful source for saliva detection, DNA extraction and profile generation. Difference of saliva accumulation on cigarette butt paper and filter was compared in the present study. On smoked cigarette butts, gender based comparison of saliva detection and DNA quantification was performed.
Agarose gel assay was used for amylase enzyme detection. All samples showed positive results for saliva detection. Cigarette butt filter and filter paper were processed separately and analyzed. Results showed more saliva detected at cigarette butt paper. Then gender based comparison on cigarette butt samples smoked by males and females was done for salivary amylase. By using student t-test, no significant difference was found on basis of gender. For extraction of DNA, phenol chloroform extraction method was used. Quantifiler® Human DNA Quantification kit was used through RT-PCR for quantification of DNA isolated from cigarette butt papers. SDS software analyzed the data and gave results of quantified DNA in ng/µL. Gender based comparison in DNA quantity was done by using statistical method. SPSS software was used for statistical analysis. Pearson correlation value was calculated between detected saliva and quantified DNA of samples. Weak positive results of correlation were obtained between saliva and DNA.
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