Brucellosis is one of the chronic infectious bacterial diseases of farm animals and man caused by Brucella specie and has a wide host range including bovine, ovine, caprine, canine, equine and man. It causes heavy economical losses in livestock holdings by resulting abortion and infertility while from public health point it is a bacterial zoonotic diseases causing undulant fever in man. In the modern world it is still a challenging health ailment to livestock and public health. In Pakistan it is prevalent in livestock holdings and human population. Keeping in view the importance of Brucellosis the present study was designed to investigate its status in District Quetta of Balochistan province.
In this study sheep, goat and their concerned (farmers, veterinary assistant, veterinarian, butchers) were included. Govt. farms, private farms and slaughter houses were used as a source of samples. Information about risk factors in man and animals were gathered by separate structured questionnaires. To investigate Brucellosis, blood and milk samples were collected and serum from blood samples was isolated. Serum and milk samples were screened with Rose Bengal Plate test and Milk Ring Test respectively, and positive cases from RBPT were further tested on Polymerase chain reaction for confirmation.
The overall seroprevalence of Brucellosis in animals was 2.40 %, 3.80 %, and 2.22 % in sheep, goat and livestock owners respectively by RBPT and this result was confirmed by PCR, while prevalence of brucellosis was 1.6% by MRT achieved. The seroprevalence of brucellosis from Lahore was 3.91% by Rose Bengal Plate test and confirmed through PCR. Among the risk factors in human significant association was found between the occupations of the person. And brucellosis is a serious threat to man. Therefore, routine surveillance of farm flocks is essential, to know about the brucellosis and local infected areas, before implementing a wide scale national programme of brucella control and eradication. There is an increasing need of educating farmers and farm managers about brucellosis and its public health significance.
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