There are various type of tumors associated with dog (Canis familiaris) and cat (Feline catus) which are responsible for death of these pets. Bcl-2 proto-oncogene was firstly depicted as of the t(14;18) trans-location cut-off point inside human follicular B-cell lymphoma. The Bcl-2 protein is a core control device of planed cell death as well as is concerned within DNA transformation, cell-cycle and differentiation control. Bcl-2 expression within endothelial cells was described en route for enhance cancer metastasis. Mammary gland tumors are the mainly frequent neo-plasms happening into feminine dogs and cats and are malevolent inside more or less 50% of the cases. Bcl-2 expression is not merely interrelated through an enhanced expression but as well by means of an abridged aptitude on behalf of far-away immigration of mammary gland cancer cells. Metastasis to tissues like skin, nasal passage and oral cavity has also been reported in 5-6.9 percent of cases.
Various parameters, used in the present study were aimed to analyze coding regions of Bcl-2gene to study the mutations involved in cancers. Blood samples of unrelated true representative of cancers were collected from Pet center, University of Veterinary and Animal Sciences, Lahore. DNA was extracted with the standard protocol and amplification of the Bcl-2 gene was done with specially designed primers.
Later on, analysis of the results was done by sequencing of amplicons. Sequences were analyzed through BioEdit software and then aligned with reference sequence using clustalW2 software.
In the present study, analysis of mutations was done in Bcl-2gene isolated from Canis familiaris and Feline catus. But not a single nucleotide polymorphism was found in exon 1 and 2 of Bcl-2 gene isolated from blood of affected animals with different cancer types.
In the conclusion, we report that no mutations were observed in the Bcl-2 gene isolated from different affected pets. It may be due to limited number of samples and/or require extraction of DNA from tumor tissue. There is a need to explore the other gene mutations causing cancers in population of pets that will ultimately help to develop genetic counseling strategies, gene therapies and prenatal diagnostic procedures for the population of Pets.