Genotoxic And Mutogenic Study Of Formaldehyde, Sodium Hypochlorite And Cresol
By: Ann Fatima (2012-VA-995)
| Prof.Dr. Muhammad Ashraf.
Contributor(s): Dr. Muhammad Adil Rasheed | Dr. Imran Altaf.
Material type: 
BookPublisher: 2015Description: 91p.Subject(s): Department of Pharmacology and ToxicologyDDC classification: 2305-T Dissertation note: Disinfectants are used to control, prevent or destroy harmful microorganisms on inanimate surfaces. These chemicals are being used to dispose the contagious hospital wastes like disposable plastics and microbiological waste. Various factors like temperature, contact period concentration of disinfectant, organic soil and nature of water used for dilution affect of disinfection process. So, disinfectants must be tested prior to any specific applications for its proper effectiveness. This study has been designed to study the genotoxicity and mutagenicity of three commonly used disinfectants, formaldehyde, sodium hypochlorite and cresol alone and in combination.
Different dilutions of formaldehyde (1, 0.3, 0.1, 0.006, and 0.003%) sodium hypochlorite (8, 4, 2, 1, and 0.5%) and cresol (7.6, 3.8, 1.9, 0.95, and 0.475%) alone and in combination were investigated for mutagenicity as well as genotoxicity in vitro. Mutagenicity was investigated by Ames Salmonella/Microsome assay with and without metabolic activation system; S-9 with the help of two strains of Salmonella typhimurium, TA 100 and TA 98 and genotoxicity was checked by Comet assay using peripheral blood lymphocytes. The results were analyzed by statistical package of Social Sciences; results were presented as mean ± S.D and the data analysis was done by using one-way analysis of variance. Differences were considered significant at P < 0.05.
Summary
83
Formaldehyde, sodium hypochlorite & cresol showed significantly mutagenic potential against TA 100 & TA 98 strains of Salmonella with and without metabolic activation system and genotoxic effects. A higher concentration showed more significant results.
Formaldehyde, sodium hypochlorite & cresol has both mutagenic and genotoxic potential. But this mutagenicity and genotoxicity has been observed more with higher concentrations as compared to low concentration.
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 2305-T (Browse shelf) | Available | 2305-T |
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Disinfectants are used to control, prevent or destroy harmful microorganisms on inanimate surfaces. These chemicals are being used to dispose the contagious hospital wastes like disposable plastics and microbiological waste. Various factors like temperature, contact period concentration of disinfectant, organic soil and nature of water used for dilution affect of disinfection process. So, disinfectants must be tested prior to any specific applications for its proper effectiveness. This study has been designed to study the genotoxicity and mutagenicity of three commonly used disinfectants, formaldehyde, sodium hypochlorite and cresol alone and in combination.
Different dilutions of formaldehyde (1, 0.3, 0.1, 0.006, and 0.003%) sodium hypochlorite (8, 4, 2, 1, and 0.5%) and cresol (7.6, 3.8, 1.9, 0.95, and 0.475%) alone and in combination were investigated for mutagenicity as well as genotoxicity in vitro. Mutagenicity was investigated by Ames Salmonella/Microsome assay with and without metabolic activation system; S-9 with the help of two strains of Salmonella typhimurium, TA 100 and TA 98 and genotoxicity was checked by Comet assay using peripheral blood lymphocytes. The results were analyzed by statistical package of Social Sciences; results were presented as mean ± S.D and the data analysis was done by using one-way analysis of variance. Differences were considered significant at P < 0.05.
Summary
83
Formaldehyde, sodium hypochlorite & cresol showed significantly mutagenic potential against TA 100 & TA 98 strains of Salmonella with and without metabolic activation system and genotoxic effects. A higher concentration showed more significant results.
Formaldehyde, sodium hypochlorite & cresol has both mutagenic and genotoxic potential. But this mutagenicity and genotoxicity has been observed more with higher concentrations as compared to low concentration.
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