Genetic Effect Of Cholesteryl Ester Transfer Protein (Cetp) Gene In Coronary Heart Disease Patients
By: Zakiya Bano (2013-VA-554)
| Dr. Akhtar Ali.
Contributor(s): Dr. Waseem Shehzad | Dr. Muhammad Tayyab.
Material type: 
BookPublisher: 2015Subject(s): Department of Molecular Biology and BiotechnologyDDC classification: 2345-T Dissertation note: Cholesteryl ester transfer protein (CETP) gene takes part with certain reverse cholesterol
transport (RCT) pathway for the excess amount of accumulated lipid in peripheral tissues. The
variations in this gene due to missense mutations on different exonic, intronic or on promoter
regions alter CETP activity as well as impair the RCT pathway. By which, lipid metabolism also
effects and causes atherosclerosis in vessels which trigger the blockage of blood flow and induces
the imbalance for the supply of oxygen to the heart. So this atherosclerosis directly involves in
addition of risk factor for coronary heart disease. Preferable study was made to highlight effect of
CETP gene at molecular level by comparing control group with the selected patients having
coronary heart disease. This study was appreciably made possible by targeting two reported
polymorphisms, one in the intron 1 region Taq IB (rs708272) and on exon 14 region I405V
(rs5882) of this CETP gene. The study was relatively speculated by the extraction of genomic
DNA from all selected blood samples. By selecting two primers, certain segments were amplified
for both rs708272 and rs5882 polymorphisms. Analysis of allelic frequencies distribution was
calculated by Hardy Weinberg Equilibrium which showed no significance among control and
CHD group and there was no association was analyzed in our population by using Fisher’s Exact
Test. This is because of small number of samples studied in our population. But maximize
concentrations of lipid parameters such as TC, LDL and TG with minimum variation in HDL-C
concentration in CHD group as compared to control group that showed the effect of these
polymorphisms on the activity of CETP gene with coronary heart disease. These determined
missense mutations in CETP gene was helpful molecular tool for the screening purpose in coronary
heart disease patients.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|---|
Thesis
|
UVAS Library Thesis Section | Veterinary Science | 2345-T (Browse shelf) | Available | 2345-T |
Cholesteryl ester transfer protein (CETP) gene takes part with certain reverse cholesterol
transport (RCT) pathway for the excess amount of accumulated lipid in peripheral tissues. The
variations in this gene due to missense mutations on different exonic, intronic or on promoter
regions alter CETP activity as well as impair the RCT pathway. By which, lipid metabolism also
effects and causes atherosclerosis in vessels which trigger the blockage of blood flow and induces
the imbalance for the supply of oxygen to the heart. So this atherosclerosis directly involves in
addition of risk factor for coronary heart disease. Preferable study was made to highlight effect of
CETP gene at molecular level by comparing control group with the selected patients having
coronary heart disease. This study was appreciably made possible by targeting two reported
polymorphisms, one in the intron 1 region Taq IB (rs708272) and on exon 14 region I405V
(rs5882) of this CETP gene. The study was relatively speculated by the extraction of genomic
DNA from all selected blood samples. By selecting two primers, certain segments were amplified
for both rs708272 and rs5882 polymorphisms. Analysis of allelic frequencies distribution was
calculated by Hardy Weinberg Equilibrium which showed no significance among control and
CHD group and there was no association was analyzed in our population by using Fisher’s Exact
Test. This is because of small number of samples studied in our population. But maximize
concentrations of lipid parameters such as TC, LDL and TG with minimum variation in HDL-C
concentration in CHD group as compared to control group that showed the effect of these
polymorphisms on the activity of CETP gene with coronary heart disease. These determined
missense mutations in CETP gene was helpful molecular tool for the screening purpose in coronary
heart disease patients.
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