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Effects Of Omega 3 And Vitamin E Against Experimentally Infected Low Pathogenic Avian Influenza Virus H9n2 In Broiler Chickens

By: Muhammad Sulman Ali Taseer (2008-VA-089) | Prof. Dr. Asim Aslam.
Contributor(s): Prof. Dr. Zafar Iqbal Chaudhry | Prof. Dr. Kamran Ashraf.
Material type: materialTypeLabelBookPublisher: 2015Description: 42p.Subject(s): Department of PathologyDDC classification: 2355-T Dissertation note: Avian influenza is a highly contagious viral disease of domestic and wild birds. It is one of the most devastating viral disease of poultry industry. It was first identified in Italy in early 1900,s and is now known to exist worldwide. Total of 125 day old chickens were divided into five groups (A, B, C, D, E) with 25 chickens in each group. Group A was negative control group. In groups B, C, D and E low pathogenic avian influenza (H9N2) virus infection was introduced at day 28 of age. Group B was given with Omega 3. In group C chickens were given Vitamin. E. In group D chickens were fed both Omega 3 and Vitamin E. Group E was positive control group without any additional supplementation. At days 27, 30, 35, 42, blood was collected aseptically from wing vein, from three birds in each group to check H/L ratio and to perform HI test to check antibody titer for H9. After collection of blood five birds from each group were slaughtered to observe postmortem signs and for the histopathology of lungs and trachea. Heterophill to lymphocyte ratio was significantly high in groups D (Omega 3 and Vit.E) and group E (Positive Control). Among the various treatment groups of broilers the significantly highest HI antibody titer was recorded in group E which was positive control group. In treatment groups C (Vitamin E supplement) and D (Omega 3 and Vit.E) HI antibody titer was near to protective titer against H9. Major histopathological lesions involved deciliation of trachea and sloughing of epithelium of trachea. There was infiltration of monocytes and neutrophils as well as vascular congestion in the form of hemorrhagic areas in lungs. There was increase in congestion in the lungs of the chicks in group E (Positive Control). 37 FCR was evaluated on weekly basis. A comparatively better feed conversion ratio was recorded in group D (Omega 3 and Vit.E). There was no significant difference in feed conversion ratio of the other treatment groups.
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Thesis Thesis UVAS Library
Thesis Section
Veterinary Science 2355-T (Browse shelf) Available 2355-T
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Avian influenza is a highly contagious viral disease of domestic and wild birds. It is one of the most devastating viral disease of poultry industry. It was first identified in Italy in early 1900,s and is now known to exist worldwide. Total of 125 day old chickens were divided into five groups (A, B, C, D, E) with 25 chickens in each group. Group A was negative control group. In groups B, C, D and E low pathogenic avian influenza (H9N2) virus infection was introduced at day 28 of age. Group B was given with Omega 3. In group C chickens were given Vitamin. E. In group D chickens were fed both Omega 3 and Vitamin E. Group E was positive control group without any additional supplementation.
At days 27, 30, 35, 42, blood was collected aseptically from wing vein, from three birds in each group to check H/L ratio and to perform HI test to check antibody titer for H9. After collection of blood five birds from each group were slaughtered to observe postmortem signs and for the histopathology of lungs and trachea. Heterophill to lymphocyte ratio was significantly high in groups D (Omega 3 and Vit.E) and group E (Positive Control). Among the various treatment groups of broilers the significantly highest HI antibody titer was recorded in group E which was positive control group. In treatment groups C (Vitamin E supplement) and D (Omega 3 and Vit.E) HI antibody titer was near to protective titer against H9.
Major histopathological lesions involved deciliation of trachea and sloughing of epithelium of trachea. There was infiltration of monocytes and neutrophils as well as vascular congestion in the form of hemorrhagic areas in lungs. There was increase in congestion in the lungs of the chicks in group E (Positive Control).
37
FCR was evaluated on weekly basis. A comparatively better feed conversion ratio was recorded in group D (Omega 3 and Vit.E). There was no significant difference in feed conversion ratio of the other treatment groups.

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