A major nutritional problem these days is the biological and chemical contamination of food with environmental chemicals; Bisphenol A is one of these chemicals which cause contamination of food. BPA have many genomic effects, it is consider as endocrine disrupting hormone. However BPA have many non genomic effects e.g BPA enter into the body orally by use of plastic food cans lead through the intestine and liver and affects target organ. These chemicals have also shown adverse effects on development and function of the reproductive organs of the birds (Berg 2000).
Bisphenol A may affect the barrier function of small intestine of layers. Our study aimed to find out the effect of Bisphenol A on the electrophysiology of jejunum of small intestine of layers. For this purpose a total of forty layer birds were purchased from poultry farm. The birds were acclimatized for one week. Following acclimatization one bird was slaughtered daily and jejunum were collected. After stripping the jejunum was divided into four segments and was mounted in four Ussing chambers receiving four treatments as (BPA control, BPA 10 μM, BPA 50 μM and BPA 100 μM) The tissues were first incubate under open circuit conditions for 15 min and then were short circuited by fixing the voltage at 0 mV throughout the experimental period. After 5 minutes following short circuiting the tissues BPA at the concentration of 10 μM, 50 μM and 100 μM, were added on the mucosal side respectively followed by an equilibrium period of ten minutes. The tissue was further monitored for 30 minutes to note any change in electrophysiology is caused by BPA. After this 300uL of concentrated glucose solution was prepared and added to the mucosal side of each chamber to achieve the final concentration of 20mmol/L. The electrical response to glucose addition was observed. ANOVA was used to
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investigate the effect of Bisphenol A on the electrophysiology of jejunum. Followed by Tukey. The P ˂ 0.05 was considered significant.
The results of this study showed that BPA 10μM affects the electrophysiology of intestine as compared to other groups (50μM, 100μM). Suggesting a possible role of BPA in affecting intestinal barrier function and glucose uptake.