Isolation And Molecular Characterization Of Causative Agent Of Equine Strangles
By: Tayyaba Naz (2008-VA-235)
| Prof. Dr. Khushi Muhammad.
Contributor(s): Dr. Aamir Ghafoor | Prof. Dr. Aneela Zamir Durrani.
Material type: 
BookPublisher: 2015Description: 85p.Subject(s): Department of MicrobiologyDDC classification: 2445-T Dissertation note: Strangles is an important infectious and contagious disease of horses that affects upper respiratory tract. This disease is very much prevalent in Pakistan. Disease can be controlled by early diagnosis, strict quarantine measures and proper immunization. Disease is characterized by nasal discharge, submandibular lymph node swelling, raised temperature and anorexia. The disease can terminate into encephalitis, purpura hemorrhagica and bastard strangles. 20 nasal swabs as samples were collected from horses showing signs of nasal discharge or swollen lymph nodes from Remount depot Sargodha, 10 samples from clinically sick horses and 10 from apparently healthy horses with the history of disease. Samples were swabbed on to blood agar with 5% defibrinated blood of sheep. Isolated hemolytic colonies were undergone biochemical testing with the help of API strep 20 kit. Samples which appeared as streptococcus equi through biochemical testing were subjected to molecular amplification by targeting two genes. Two different PCR were performed PCR 1 targeted the Sod A gene this gene is present in Streptococcus equi and PCR-2 targetted SeM gene this gene is specific for Streptococcus equi subsp. Equi only. 5 samples were confirmed positive for Streptococcus equi through biochemical and molecular testing. SDSPAGE on the isolated bacterial samples were performed and it appeared as no protein diversity was observed among different isolates. However the protein pattern varied with number of passages as less number of bands appeared from older cultures.
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Strangles is an important infectious and contagious disease of horses that affects upper respiratory tract. This disease is very much prevalent in Pakistan. Disease can be controlled by early diagnosis, strict quarantine measures and proper immunization. Disease is characterized by nasal discharge, submandibular lymph node swelling, raised temperature and anorexia. The disease can terminate into encephalitis, purpura hemorrhagica and bastard strangles. 20 nasal swabs as samples were collected from horses showing signs of nasal discharge or swollen lymph nodes from Remount depot Sargodha, 10 samples from clinically sick horses and 10 from apparently healthy horses with the history of disease. Samples were swabbed on to blood agar with 5% defibrinated blood of sheep. Isolated hemolytic colonies were undergone biochemical testing with the help of API strep 20 kit. Samples which appeared as streptococcus equi through biochemical testing were subjected to molecular amplification by targeting two genes. Two different PCR were performed PCR 1 targeted the Sod A gene this gene is present in Streptococcus equi and PCR-2 targetted SeM gene this gene is specific for Streptococcus equi subsp. Equi only. 5 samples were confirmed positive for Streptococcus equi through biochemical and molecular testing. SDSPAGE on the isolated bacterial samples were performed and it appeared as no protein diversity was observed among different isolates. However the protein pattern varied with number of passages as less number of bands appeared from older cultures.
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