UVAS Library

Your cart is empty.

Your search returned 3 results. Subscribe to this search

Not what you expected? Check for suggestions
Unhighlight Highlight
|
1.
No cover image available
Standardization Of Multiplex Rt-Pcr For The Diagnosis Of Avian Influenza (H9) Virus Newcastle Disease Virus (Ndv)

by Asad Shahzad | Dr.Asim Aslam | DR. Jawad Nazir | DR.Raheela Akhtar.

Material type: book Book; Format: print Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1917,T] (1).

Place hold Add to cart
2.
No cover image available
Comparative Pathological Association Of Slc11a1 (Nramp1) Gene With Susceptibility And Resistance To Brucellosis In Local Pakistani Buffaloes

by Azmat Ullah(2015-VA-1067) | Dr.Raheela Akhtar.

Material type: book Book; Literary form: not fiction Publisher: 2017Dissertation note: Nearly 32.7 million population of buffaloes present in Pakistan which makes about 15% of the world buffalo population. For water buffaloes Brucellosis is a pricey disease (Bubalus bubalis). In Pakistan 5 known breeds of buffalos are; Ravi, Nili, Kundi Nili-Ravi and Aza Kheli. The study was conducted on 200 animals of two breeds including Nilli-Ravi and Kundi around farms of Punjab. 200 serum samples from two breeds were screened for brucellosis. Dormant infections and lengthy incubation of the pathogens bounded the effectiveness of programs based upon the elimination of infected animals. As comparative genomics is playing a pivotal role in the genetic dissection of complex traits such as infectious diseases resistance using this information we can oppressed for disease resistance with genetic choice as a method to the regulator of brucellosis in our local breeds such as Nili-Ravi buffalo. Blood sample (5mL) was collected in EDTA vacutainers from each animal. Serum from blood samples were collected in 3 mL eppendorf tubes with the help of sterile pipettes. Serum samples were screened out for brucellosis by Rose Bengal Plate Tetst (RBPT). Rose Bengal Antigen was purchased from Veterinary Research Institute (VRI), Lahore. The antigen was stored at 40C in refrigeration during the study according to manufactures recommendation. DNA quantification was performed on “Thermo Scientific NanoDrop spectrophotometer ND-2000” and by running extracted DNA on 0.8% agarose gel. Nanodrop spectrophotometer provides direct and easy measurements within a 5 second only by using just a pipette and wipe. PCR for amplification was done with a total volume of 20μl by adding primer pair and extracted DNA to the PCR master mix in following concentrations. 2μl of forward and reverse primer was taken respectively. 4 μl of PCR grade water was added and DNA was taken in 2 μl Summary 32 Quantity. The total volume of master mix obtained was 10 μl. The thermocycler (Bio-Rad, CFX ™, Singapore) was set with the conditions for the total of 35 cycles of PCR. Initial denaturation of DNA samples were performed at 940 C for 5minutes. Then in 2nd cycle denaturation was performed at 94 0C for 30 seconds. Annealing step was performed at 63 0C for 30 second. Then, initial extension of DNA samples was conducted at 720 C for 30 second. Finally, extension was performed at 720 C for 5 minutes to get results. The PCR product was confirmed by running the PCR product on 1.5 % Agarose gel electrophoresis was performed at 110 Volts for 30 minutes. In Nilli-Ravi, among 12 positive samples by PCR, 08 were found resistance to brucellosis through sequencing study of Nramp 1 BB, only one sample was found to be susceptible to brucellosis through genotypic study of Nramp1 AA and 3 samples were found to be neutral may be susceptible or resistance to brucellosis. In Kundi, among 38 positive samples by PCR, 03 were found resistance to brucellosis through sequencing study of Nramp 1 BB, and 6 sample were found to be susceptible to brucellosis through genotypic study of Nramp1 AA and 29 samples were found to be neutral may be susceptible or resistance to brucellosis. This research clearly demonstrates that Kundi is more susceptible to brucellosis then Nilli-Ravi. Conclusion: We concluded that Nilli-Ravi is more resistant to brucellosis as compare to Kundi breed Natural resistance against brucellosis in cattle is linked to the Nramp 1 gene. Polymorphisms at the bovine Nramp 1 3/ untranslated region (3 UTR) are associated with natural resistance against brucellosis. Availability: No items available

Add to cart
3.
No cover image available
Pathological Association Of Nramp 1 Genotypes With Brucella Resistance And Susceptibility In Diseased And Non Diseased Cattle

by Muhammad Zaheer Iqbal(2005-VA-61) | Dr.Raheela Akhtar.

Material type: book Book; Literary form: not fiction Publisher: 2017Dissertation note: A total of 200 cattle were divided into five groups including: Group A Sahiwal cattle, Group B Jersy cattle, Group C Frisian cattle, group D Sahiwal cross Jersy and group E Sahiwal cross Frisian. Out of total of 200 serum samples from suspected cattle we found 155 samples positive by RBPT and 109 were positive for Brucella abortus by PCR. Comparison of presence of Brucella abortus was statically made in all five groups using chi square. The study was conducted on 200 animals of five breeds including Sahiwal, Jersey Cross Sahiwal, Frisian Cross Sahiwal, Fresian and Jersey around farms of Punjab. Blood sample (3mL) was collected in EDTA vaccutainers from each animal. Serum from blood samples were collected in 3 mL eppendorf tubes with the help of sterile pipettes. Serum samples were screened out for brucellosis by Rose Bengal Plate Test (RBPT). RBPT positive samples were stored at 40C for further processing. Rose Bengal Antigen was purchased from Veterinary Research Institute (VRI), Lahore. The antigen was stored at 40C in refrigeration during the study according to manufactures recommendation. DNA quantification was performed on “Thermo Scientific NanoDrop spectrophotometer ND-2000” and by running extracted DNA on 0.8% agarose gel. PCR for amplification was done with a total volume of 20 μL by adding primer pair and extracted DNA to the PCR master mix in following concentrations. 2ml of forward and reverse primer was taken respectively. 4ul of PCR grade water was added and DNA was taken in 2 ul quantity. The total volume of master mix obtained was 10 ul. The thermocycler (Bio-Rad, CFX ™, Singapore) was set with the conditions for the total of 35 cycles of PCR. For optimization process of primers different options for PCR reaction mixture Summary 41 and PCR cyclic conditions were tried for two objectives. To get maximum amplification, by using minimum volume of chemicals. Changing the volume of magnesium chloride, deoxynucleotide triphosphate (d NTPs) and Taq polymerase, amplification can be increased. Primers annealing temperature is considered critical for optimization. Denaturation of DNA samples were performed at 94 0C for 5minutes. Annealing step was performed at 63 0C for 30 second. Then, initial extension of DNA samples was conducted at 72 0C for 30 second. Finally, extension was performed at 72 0C for 5 minutes to get results. The PCR product was confirmed by running the PCR product on 0.8 % agarose gel electrophoresis was performed at 100 Volts for 30 minutes. The NRAMP1 gene encodes a divalent cation transporter, located in the phagolysosomal membrane of macrophages, which has been associated with resistance to intracellular pathogens. In cattle, natural resistance against brucellosis has been associated with polymorphisms at the 3′ untranslated region (3′UTR) of the NRAMP1 gene, which are detectable by single-strand conformational analysis (SSCA). Genetic selection of domestic animals resistant to pathogens has been applied mostly to farm animals, particularly cattle. Identification of genes linked to natural resistance may allow for a better understanding of natural resistance with obvious practical implications. These genes may also function as markers for prediction of genetic resistance against specific diseases. Recommendations: From this study we concluded that Nramp1BB gene is resistant to brucellosis, while Nramp1 AA is susceptible to brucellosis. Summary 42  By gene knock out technique breeds resistant to brucellosis can be produced.  Criss Crasper technique can be used for gene knock out process Availability: No items available

Add to cart

Implemented and Maintained by UVAS Library.
For any Suggestions/Query Contact to library or Email:[email protected] Phone:+91 99239068
Website/OPAC best viewed in Mozilla Browser in 1366X768 Resolution.