51.
Effect Of Rumen Protected Lysine And Methionine On Production Performance And Blood Metabolites Of Early Lactating
by Madiha gohar | Dr. Saeed ahmad | Dr. Nisar ahmad | Prof .Dr. Anjum khalique.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2074,T] (1).
52.
Application Of Multivariate Principal Component Analysis For The Morphological Characterization Of Cholistami Cattle
by Waseem Abbas Shah | Dr. Nisar Ahmad | Ne. Muhammad Saudullah | Prof.Dr.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2141,T] (1).
53.
Comparative Efficacy Of Flank And Midline Approach For Nephrotomy In Dogs
by Aamir Noor | Prof.Dr. Muhammad Arif | Dr. Jawaria Ali Khan | Dr. Sadaf Aslam.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2152,T] (1).
54.
Effect of Timing of Artificial Insemination After Controlled Internal Drug Release (CIDR) Device Removal And Or CIDR + GnRH Based Estrus Synchronization Protocols In Nili Ravi Buffalo
by Muhammad Shafiq Haider (2008-VA-226) | Prof. Dr. Naseem Ahmed | Mr. Muhammad Usman Mehmood | Prof.Dr. Muhammad Sarwar Khan.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Blank CD Availability: Items available for loan: UVAS Library [Call number: 2240-T] (1).
55.
Effect of Estradiol Benzoate (EB) and Equine Chorionic Gonadotropin (eCG) on Ovarian Response and Conception Rate in Control Internal Drug Release (CIDR) Based Estrus Synchronization Protocol in Nili Ravi Buffalo
by Abdul Saboor Khan (2008-VA-116) | Prof. Dr.Nasim Ahmad | Prof.Dr. Mian Abdul Sattar | Prof. Dr. Aneela Zameer Durrani.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Blank CD. Availability: Items available for loan: UVAS Library [Call number: 2239-T] (1).
56.
Effect Of Different Vaccine Routes On Growth Performance, Organs Weight, Carcass Characteristics And Immune Response In Broilers
by Habiba Taj (2008-VA-478) | Prof.Dr. Athar Mahmud | Dr. Shahid Mehmood | Dr. Atia Bashir.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: The purpose of study was to compare four different vaccination routes. The study was conducted at environment controlled broiler house, University of Veterinary and Animal Sciences, Ravi campus-Pattoki. In this experiment 264 commercial day old chicks were purchased from hatchery.On arrival, 24 day-old chicks were sent to laboratory for determining the maternal antibody level and remaining 240 birds were divided randomly in to four treatments (Drinking water, Eye drop, Spray,Spray +Drinking water) groups. Each group was replicated 6 times with 10 birds each. Before vaccination 03 birds were picked up randomly from each replicate and 3 ml blood was collected from the main brachial vein to determine NDV and IBV maternal antibody titers of the chickens. At the end of the trial, 03 birds were picked up randomly, slaughtered to determine the weight of immune organs and carcass characteristics. The data thus obtained were statistically analyzed through Completely Randomized Design (CRD) using one way analysis of variance (ANOVA) techniques at 0.05 significane level. The means were compared using Duncan’s Multiple Range (DMR) test with the help of SAS, 9.1.Results of present study showed non-significant differences (P ≥ 0.05) infeed intake, body weights, feed conversion ratio, feed efficiency, point spread, performance index andproduction number. Significant differences (P ≤0.05) were observed in mortality and livability percentage. The birds vaccinated thorugh the eye drop showed higher livability than spray with drinking water.Significant differences (P ≤ 0.05) were observed inBursa and thymusweights with respect to four routes of vaccination. The birds vaccinated through eye drop method had the higher bursa and thymusweight as compared to those vaccinated through drinking water, eye drop and spray with drinking water. There were non-significant (P ≥ 0.05) in spleen weight.Maternal antibody level against Infectious Bronchitis and Newcastledisease was 3421and 6.9 respectively. Decline in maternal antibody titer was recorded onthe 7th and 15th day of the experiment.Vaccination at 17th day boosted the immune response.Significant differences (P ≤ 0.05) were observed in an immune response against the Infectious Bronchitis and Newcastle Disease. The birds vaccinated through eye drop route showed higher immune response than other routes of vaccination.Non-significant differences (P ≥ 0.05) were observed in dressing percentage, breast yield percentage, gizzard percentage, heart percentage, keel length, shank length, intestinal length and intestinal weight. Significant differences (P ≤0.05) were observed in thigh percentage.
Conclusion:
Based on the findings of the present study it may be stated that
1. Vaccination routes had no effect on growth performance.
2. Eye drop vaccinataion developed better immunity and also had higher weight of immune organs, while, spray remained poorest.
Suggestion and Recommendations:
For future it is recommended as eye drop method of vaccination is the best route of vaccination for higher immune response and higher immune organs weight and others routes of vaccinations has lower immune response and imuune organs weight. Continuous detailed and comprehensive research investigations are needed on different vaccination routes.
Availability: Items available for loan: UVAS Library [Call number: 2271-T] (1).
57.
Genotoxic And Mutogenic Study Of Formaldehyde, Sodium Hypochlorite And Cresol
by Ann Fatima (2012-VA-995) | Prof.Dr. Muhammad Ashraf | Dr. Muhammad Adil Rasheed | Dr. Imran Altaf.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Disinfectants are used to control, prevent or destroy harmful microorganisms on inanimate surfaces. These chemicals are being used to dispose the contagious hospital wastes like disposable plastics and microbiological waste. Various factors like temperature, contact period concentration of disinfectant, organic soil and nature of water used for dilution affect of disinfection process. So, disinfectants must be tested prior to any specific applications for its proper effectiveness. This study has been designed to study the genotoxicity and mutagenicity of three commonly used disinfectants, formaldehyde, sodium hypochlorite and cresol alone and in combination.
Different dilutions of formaldehyde (1, 0.3, 0.1, 0.006, and 0.003%) sodium hypochlorite (8, 4, 2, 1, and 0.5%) and cresol (7.6, 3.8, 1.9, 0.95, and 0.475%) alone and in combination were investigated for mutagenicity as well as genotoxicity in vitro. Mutagenicity was investigated by Ames Salmonella/Microsome assay with and without metabolic activation system; S-9 with the help of two strains of Salmonella typhimurium, TA 100 and TA 98 and genotoxicity was checked by Comet assay using peripheral blood lymphocytes. The results were analyzed by statistical package of Social Sciences; results were presented as mean ± S.D and the data analysis was done by using one-way analysis of variance. Differences were considered significant at P < 0.05.
Summary
83
Formaldehyde, sodium hypochlorite & cresol showed significantly mutagenic potential against TA 100 & TA 98 strains of Salmonella with and without metabolic activation system and genotoxic effects. A higher concentration showed more significant results.
Formaldehyde, sodium hypochlorite & cresol has both mutagenic and genotoxic potential. But this mutagenicity and genotoxicity has been observed more with higher concentrations as compared to low concentration. Availability: Items available for loan: UVAS Library [Call number: 2305-T] (1).
58.
Detection Of Prevalent Strain Of Ppr Virus And Efficacy Of Imported Live Attenuated Ppr Vaccine In Local Goat In Pakistan
by Iqra Javaid (2008-VA-76) | Prof.Dr. Aneela Zameer Durani | Dr.M. Hassan Saleem | Dr. Imran Altaf.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Peste des petits ruminants (PPR) is a viral, extremely transmissible disease with 100% and 90% of morbidity and death rate in small ruminants (Singh et al. 2004; Singh et al. 2009). The morbillivirus of the family Paramyxoviridae is responsible for its etiology (Barrett et al. 2005). The clinical signs of Peste des petits ruminants (PPR) are severe pyrexia, oculo-nasal discharge, necrotizing and erosive stomatitis, enteritis and pneumonia (Dhar et al. 2002) and is also accompanied by decrease in lymphocyte count (Rajak et al. 2005). Peste des petits ruminants (PPR) produces a major impact on the economy of the country (Zahur et al. 2009). Because of huge economic blow, the Peste des petits ruminants (PPR) imposes a major limitation on sheep and goat production (Asim et al. 2009; Abubakar and Munir. 2014).
The homologous Peste des Petits Ruminants (PPRV) vaccines using Nigeria 75/1 strain of the virus are being manufactured in Pakistan (Asim et al. 2009). The Advance Studies in Vaccinology and Biotechnology Center (CASVAB) University of Baluchistan, Quetta, with the help of Vero cell line manufactured the freeze dried and tissue culture based PPR virus (PPR 75-1) vaccine (Abbas et al. 2011). The homologous and Vero cell based live attenuated PPR vaccine having origin of Indian virus isolate “PPRV-Sungri/96”is being manufactured in India for immunization against Peste des Petits Ruminants (PPR) disease (Sreenivasa et al. 2000).
Twenty goats of different age, breed and sex were examined for the presence of PPR disease during this study. About 2-3 ml of saliva was collected from oral cavity of twenty PPR suspected goats in falcon tubes, signifying PPR disease. The extraction of RNA from the samples was done by trizole method and the concentration was measured by nanodrop. The extracted samples were then subjected to one step RT-PCR and then the PCR products were sent for sequencing to detect the PPRV strain under field conditions.
To study immunogenic behavior of Raksha PPR (Sungri 96), total of forty (40) goats free from peste des petits ruminants virus (PPR-V) were selected for the experimental study. The Group A comprising of twenty (20) goats of age (06 months-01 Year) were further subdivided into two groups i.e subgroup A1 comprising of 10 goats to which Raksha PPR vaccine (Sungri 96) was administered and other ten of sub-group A2 served as control. Similarly the Group B possessing twenty (20) goats of age (01 Year - 02 Year) were further subdivided into two sub-groups i.e subgroup B1 comprising of 10 goats to which Raksha PPR vaccine (Sungri 96) was administered and other ten of sub-group B2 served as control.
The RNA concentration was different in all twenty saliva samples when measured by nanodrop. Only five (5) samples out of total twenty (20), saliva samples from PPR suspected goats, were positive through RT-PCR and yielded an amplified product of 351bp. The five amplicons were sent for sequencing and the phylogenetic tree was constructed. The tree demonstrated that the Pakistani strains of PPRV clustered into lineage IV showing similarity with the isolates from China, Kurdistan, Iran and Bangladesh.
It was revealed that the that the animals (1 year to 2year old ) vaccinated with Raksha PPR (Sungri 96) displayed the significantly higher mean antibody titers than the mean antibody titers shown by vaccinated animals of age (6 months to 1 year) at zero, 7th, 14th, 28th, 48th day post vaccination respectively. On statistical analysis of data, the results were significant (p<0.05).
The present study revealed the presence of lineage IV in Pakistan. This will help to plan proper control strategies against this deadly viral disease. Currently the Nigeria75/1 vaccine is being used in Pakistan which clusters in lineage II while Pakistani field isolates fall under lineage IV. So it is very important to immunize the animals with lineage specific vaccine like Raksha PPR (Sungri 96) manufactured by IVRI, India.
This study reported the strong association of age and PPR vaccination titer in goats. Our findings concluded that the strong immune response was shown by adult animals against PPRV vaccine as compared to young stock. The results of present research project were mostly similar with the findings of other scientists. The results of this study were analyzed by one way ANOVA for independent samples.
Availability: Items available for loan: UVAS Library [Call number: 2381-T] (1).
59.
Khawateen Ki Zimadari
by Prof.Dr. Fazal Ellahi.
Edition: 1stMaterial type: ; Literary form:
not fiction
Publisher: Lahore: Darul Salam; 2016Availability: Items available for loan: UVAS Library [Call number: 297 Ellahi 31591 1st 2016 Islam] (1).
60.
Efects Of Feeding Mannanoligosaccharides On Serum Minerals Profile In Beetal Goats
by Zil-E-Huma (2014-VA-11) | Dr. Muhammad Shabaz Yousaf | Prof .Dr. Imtiaz Rabbani | Dr. Saif-ur-Rehman Kashif.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: The need of minerals is increased during gestation and lactation to support fetal growth and milk production respectively and prebiotics are considered to increase mineral absorption during gestation. Mannan-oligosaccharide supplementation can enhance minerals absorption during pregnancy. The study was conducted in the experimental shed of University Of Veterinary and Animal Science, Pattoki for a period of 60 days. Total of 24 pregnant goats were taken and kept under normal temperature. The pregnant goats will be divided into 3 groups. Group-I containing 8 pregnant goats served as control group and throughout the experiment basal feed was given to this group. Group-II and III served as experimental groups each containing 8 pregnant goats. In Group-II and III eight pregnant goats were given 5g and 10g manan-oligosaccharide with basal feed, respectively. Sampling was done after one week of MOS supplementation and before 2 weeks of parturition. The research data was evaluated by two-way analysis of variance (ANOVA). This study helped to evaluate importance of prebiotics (MOS) in mineral absorption during gestation. The results of minerals concentration in pregnant goats with basal diet revealed that without supplementation of mannan-oligosaccharides no significant effect on serum minerals was observed.In our study mannan-oligosaccharides supplementation significantly modulated calcium and iron serum levels rest of the minerals including copper and zinc did not showed any significant modulations in their concentrations under the effect of mannan oligosaccharide supplementation. The supplementation of MOS at 5g and 10g increased serum calcium concentration (P < 0.05) while the status of the group, before birth and after birth did not showed significant effect (P > 0.05) on serum calcium concentration. The supplementation of MOS at 5g and 10g increased serum iron concentration (P < 0.05) while the status of the group, before birth and after birth also showed significant effect (P < 0.05) on serum iron concentration. Zinc and copper concentration were not modulated under the effect 5g and 10g MOS supplementation (P > 0.05). The serum concentration of zinc and copper were not influenced by group status of before and after parturition (P > 0.05).
Availability: Items available for loan: UVAS Library [Call number: 2774-T] (1).
61.
Effect Of Age, Sex And Chilling Method On Meat Quality Traits Of Indigenous Goats
by Muhammad Umair Farid (2010-VA-221) | Mr. Jamal Nasir | Dr. Muhammad Hayat Jaspal | Prof .Dr. Aftab Ahmad Anjum.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: Agriculture sector of Pakistan is crucial for the economy of the country, livestock constitutes major part of it. Livestock products like meat and milk are important source of protein for the human beings. With the rise in incomes and increasing urbanization, use of animal based protein source is increasing at a faster rate. Mutton in general and goat meat in particular is being preferred by the local consumers as well as by export destinations like Middle East. There are certain quality issues associated with goat meat like discoloration and toughening of meat. These problems are linked with age and sex of animal and processing factors like chilling. As the animal age, sex and chilling rate has significant effect on ultimate goat meat quality parameters like color, cooking loss and tenderness. So it is required to optimize age, sex and chilling rate in order to improve goat meat quality. Present study was designed to investigate the effect of age, sex and chilling regime of goat carcasses.
Animals were procured from Local animal market (mandi) and then slaughtered according to Halal standard method. After slaughtering, carcass were split into two halves longitudinally and one half for delayed and another for rapid chilling. Temperature and pH of the sirloin muscle were measured by inserting pH meter and food grade thermometer. For color determination Minolta® chroma meter was used and for tenderness determination texture analyzer was used.All the collected data was analyzed by using t-test under Complete Randomized Design and level of significance among different interaction were studied by using One Way ANOVA with the help of SAS 9.1. Results showed that,rapid chilling caused sudden fall in temperature but the rate of pH fall was slower than the delayed chilled carcass. After 2hour of slaughtering, rapidly chilled milk teeth animals had lower carcass temperature as compare to two teeth. While in case of sex, female goats had lower carcass temperature in contrast to male. Results revealed that age either
milk or twoteeth animals had non-significant effect on Warner Bratzler Shear Force (WBSF) values. Similarly color measurement also had no effect of age except lightness (L*), which was higher in milk teeth than two teeth animals. Animal sex had no effect on Warner Bratzler Shear Force (WBSF) and color values like lightness (L)*, redness (a*), yellowness (b*), chroma (C*) and hue angle (h). While sex have higher cooking loss % in male as compare to female. Chilling method directly related to the mean tenderness value.As, rapid chilling cause toughness of meat due to the difference in temperature and pH fall as compare to delayed chilling. In case of rapid chilling, mean Warner Bratzler Shear Force (WBSF) values were higher as compare to carcass which were subjected to delay chilling.
Based on the results of the present study, it has established fact that chilling has major effect on the quality of meat. Rapid chilling can lead to cold shortening of meat and as a result the end product could become tougher. While delayed chilling leads to more tender meat. As far as age of the animal is concerned, there is non-significant difference between milk teeth and two teeth goats. It is also common in Pakistan that consumers like to eat mutton from milk teeth and two teeth goats. However, mutton obtained from goats older than two teeth need investigation regarding meat quality. In the same way, exporters are exporting goat carcasses of milk and two teeth animals but the chilling method adopted by processors in not proper. It is common practice that due to shortage of time, processors do not allow the carcasses to stay for pre-chilling and put the carcasses in chillers directly. Ultimately the carcasses lead to cold shortening and consumers’ complaint about the tenderness of the meat. Secondly the age of the goat is directly linked with lightness of meat color, i.e. older animals have higher myoglobin content and this is the reason that older animals have dark colored meat.
Availability: Items available for loan: UVAS Library [Call number: 2796-T] (1).
62.
Jnwaro kay Motadadi Amraaz
by Prof.Dr M.Maqsood.
Edition: 1st.ed:Material type: ; Literary form:
not fiction
Publisher: Islamabad: Firooz sins Private Limited 1997Availability: No items available
63.
Identification Of Pkhd1 Gene Mutation In Polycystic Kidney Disease And In-Silico Molecular Characterization In Different Mammals
by Taslim Un Nisa (2015-VA-1105) | Dr. Wasim Shehzad | Dr Muhammad Yasir Zahoor | Prof.Dr. Aftab Ahmad Anjum.
Material type: ; Literary form:
not fiction
Publisher: 2017Dissertation note: Fibrocystin is a large, receptor-like protein that is involved in the tubulogenisis and maintenance of duct-lumen architecture of epithelium. Fibrocystin has a combination with the primary cilia of epithelial cell. Renal tubules (small tube) of kidney where urine is formed lined by tiny hair like projection.
Twenty five suspected patient was selected and DNA extracted through organic extraction method from the suspected patient blood. Primers were designed PKHD1gene’s coding sequence located at 6p12.2 in human. The coding region sequenced using the ready mate terminator Sequencing Reaction Kit by Perkin Elmer/ABI and read in an automated sequencer. The allele’s variants have been only reported for Fibrocystin protein in human.
All of the sequences are evaluated by using Chromas and Bioedit software for sequence analysis. The in-silico protein analysis is done for normal and mutated alleles through UCSC and RAPTROX. Homology analysis was also done between human and mammals DNA sequence.
We found mutations which are associated with ARPKD disease and these variants are most common in other population whereas we also found some new variants. There are some reported mutations which we found in our study such as (c3790C>T),(c3891G>T),(c3790C>T). We found three new mutations in PKHD1 gene. The new mutations which we found are (c3681G>A),(c3804C>T),(c3931A>C).These mutations (c3790C>T) and (c3931A>C) in the exon 32 show significant effect on the gene and protein function. Geneticanalysis of PKHD1gene show thatPakistanifamilies have mutations as compared to other population along with some common exonic regions such as exon 32 whichisalsodescribed by others in two different studies.We also analyze the pedigrees of these patients which are consanguineous families and autosomal recessive polycystic disease.
We found total six mutations in this gene including missense/ synonymous mutations. In which, three novel mutations and others are reported mutations. These variations from the results are due to the population and consanguineous families’ pattern.In our study, we also found that Mouse and Chickencan preferably be used as a modal organisms in pathology.This study will also help us in the development of molecular genetic testing for their detection in Pakistani families and population.
Availability: Items available for loan: UVAS Library [Call number: 2941-T] (1).
