1.
Imunization Of Rabbits Against Boophilus Microplus Using Midgut And Salivary Glands
by Mohammad Asif | Dr. Khalid Saeed | Dr. H. A. Hashmi | Dr. Khushi | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2002Dissertation note: The current research was conducted as a simulation model in rabbits for the development of vaccines against cattle ticks Boophilus micro/us using tick salivary glands & midgut as the sources of antigens. The comparative efficacy of these vaccines was evaluated on the basis of antibody titres and tick rejection on the host in terms of mortality, decrease in egg laying and decrease in hatchability of eggs. It was found that the vaccine prepared from the midgut of B. microplus gave better results than the vaccine prepared from salivary glands. The highest percentage of dead ticks was observed in animals which were given midgut vaccine (21.82%). This vaccine also caused a significant decrease in the egg laying capacity of the ticks (35.83%). However, none of the vaccines had any significant effect on the hatchability of eggs laid by the ticks. The highest antibody titers were observed in rabbits injected with midgut vaccine (GMT=5.50), which was 45.28% higher than rabbits given salivary gland vaccine. The overall success rate for midgut vaccine, in terms of tick mortality on host, decrease in egg laying capacity and antibody titer,. was 37.06% for midgut vaccine while only 16.51% for salivary gland vaccine. The results are very encouraging and it is hoped that with the original host i.e. cattle, more satisfactory results can be achieved.
Availability: Items available for loan: UVAS Library [Call number: 0771,T] (1).
2.
Effect Of High Dietary Fat On Serum Cholesterol And Fatty Liver Syndrome In Broiler
by Imran Ahmed Qureshi | Dr. Zafar Iqbal Chaudhry | Dr. H. A. Hashmi | Prof. Dr. Nisar | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2003Dissertation note: The present study was designed to evaluate the effects of high dietary fat on serum cholesterol and fatty liver syndrome in broiler. For this purpose 90 (day-old) chicks were procured from local hatchery. They were divided into three groups A, B and C having 30 chicks each. The birds of group B were fed on diet containing plant and animal fat while birds of group C were fed on diet containing animal fat. Group A acted as control. Experimental parameters included serum cholesterol values and pathological changes in liver. The serum cholesterol values in chicks of groups B and C were higher than that of control group. Furthermore, the serum cholesterol value was greater in birds fed on animal fat that on plant fat. Grossly the livers of group B and C were enlarged in size, paler in colour, soft in consistency, having petechial haemorrhages, deposition of fat and fibrin. The livers of group A were grossly normal. Histopathologically, livers of group B and C showed fatty infiltration, haemorrhages and mass of eosinophilic materials. The vacuoles coalesced to create clear space that displaced the nuclei to the periphery of the cell. Addition of dietary fat from animal and plant sources in the diet of broiler chicks not only resulted in increase in serum cholesterol but also in marked macroscopic and microscopic changes in liver.
Availability: Items available for loan: UVAS Library [Call number: 0866,T] (1).
3.
Identification And Chemotherapy Of Ectoparasites Of Camels (Camel Us Dromedarius) In Dera Ghazi Khan
by Muhammad Iqbal | Prof. Dr. H. A. Hashmi | Dr. Kamran Ashraf | Dr. Syed | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2005Dissertation note: The present project has been designed for identification and chemotherapy of
ecto-parasites of camels (Camelus dromedarius) from different areas of Dera-Ghazi-Khan. For this purpose 300 camels were examined during the period October to November 2004. Out of 300 camels 109(36.3%) were found infested with ecto-parasites. Two types of ectoparasites were found to be active during those days. Sixty three camels were infested with ticks. After collection these ticks were mounted for identification, with the help of their morphological characters. The only tick found to be identified was Hyalomma dromedarii and the tick infestation was found to be 21% in those areas during October-November 2004.
Similarly, 85 suspected camels were examined by skin scraping in the District Veterinary Diagnostic Laboratory, Dera-Ghazi-Khan, for identification of mites 46 camels weie found to be infested with mange mites and the mange mites infestation was 15.3%. The species of mites identified was Sarcoptes scabiei var. cameli.
For chemotherapeutic trails 60 positive ecto-parasitic camels were divided into 3 groups i.e. A, B and C, 20 camels in each group. For further procedures these groups were divided into sub-groups i.e. A (Al + A2), B (Bi + B2) and C (Cl + C2) where each sub-group contained 10 animals (camels). Al, Bi and Cl were infested with mites and A2, B2 and C2 with ticks. The group A was treated with Ivermectin (Baymec; Bayer) at dose rate of 1 m1150 kg body weight sub/cut. Similarly the group B was treated with Neguvon (0.15%) solution with spray machine. Where the group C acted as infected, non-medicated control group.
The efficacy of Baymec on 7th day was 75% and on 14th day was 80%. But the efficacy of Neguvon (0.15% solution) on 7th day was 65% and on 14th day was 75%. The camels of group C were not treated and acted as control.
Availability: Items available for loan: UVAS Library [Call number: 0895,T] (1).
4.
Comparison Of Different Diagnostic Techniques Against Fasciolosis In Buffaloes
by Muhammad Mutee-us-Salam | Prof. Dr. H. A. Hashmi | Dr. Azhar Maqbool | Prof. Dr. Zafar | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Nature of contents: ; Literary form: Publisher: 2005Dissertation note: The present study as conducted to find out the most reliable technique for the diagnosis of fasciolosis in buffaloes and to calculate economic losses due to fasciolosis. A total 0 F 1 00 faecal samples were examined by Director Smear, Zinc-sulfate flotation and Sedimentation techniques. Prevalence was 2, 4 and 5 percent respectively. Although they are very cheap and simple techniques but detection of the disease in early stages is not possible. Where as Agar Gel Precipitation technique gave positive results as 8% is laborious technique. But the diagnosis during the early stages is possible. Prevalence of Fasciolosis in Young animals (below 2 years) was found 0% Direct Smear Method. 3.33% by Zinc sulfate Flotation Technique. 6.67° by Sedimentation Technique and 6.67% by using Agar Gel Precipitation technique. In Adult (above 2 years) the Prevalence was found 2.85% by Direct Smear Method, 4.28% by Zinc sulfate Flotation Technique. 4.28% by Sedimentation technique and 8.57% by using Agar Gel Precipitation Technique. In Males the Prevalence was found 0% by Direct Smear Method. 0% by Zinc sulfate Flotation Technique, 6.26% by Sedimentation Technique and 6.26% by using Agar Gel Precipitation Technique. In Females the Prevalence was Found 2.38% by Direct Smear Method. 4.76% by Zinc sulfate Flotation Technique. 4.76% , by Sedimentation Technique and 8.33% by using Agar ( el Precipitation Technique. In Neeli Ravi the Prevalence was found 2.35% by direct Smear Method. 4.76°/o by Zinc sulfate Flotation Technique. 4.76°/o by Sedimentation technique and 8.23% by using Agar Gel Precipitation Technique. In Kundi the Prevalence was found 0% by direct Smear Method. 0° b Zinc sulfate Flotation Technique. 6.66% by Sedimentation Technique and 6.66% by using Agar Gel Precipitation Technique. From the results ii appears that AGPT Sedimentation technique, Zinc-sulfate flotation and Direct Smear Method can be ranked as No. I. 2. 3. and 4 respectively in terms of their efficacy. Total economic losses due to fasciolosis during three months (Oct.-Dec.2004) were very high i.e. Rs.1016400.
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5.
Comparative Efficacy Of Different Prophylactic & Curative Measures Against Caecal Coccidiosis In Poultry
by Muhammad Imran Rashid | Dr. H. A. Hashmi | Dr. Irshad | Mr. Wasim Shehzad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2006Dissertation note: To find out the therapeutic and prophylactic efficacy of toltrazuril against coccidiosis, an experiment was conducted in the Department of Parasitology, University of Veterinary & Animal Sciences, Lahore.
One hundred and sixty (160) day old chicks were divided into 8 groups, i-e, A, B, C, D, E, F, G and H each consisting of 20 chicks. Group A acted as non-infected and non-medicated control, B was infected with Eimeria tenella sporulated oocysts on day 16 and 26 @ 20000 per chick and acted as infected control. Similarly, all other members of different groups were also infected at the same rate. Members of Group C were administered toltrazuril orally in drinking water @ 7 mg/ kg body weight on day 18 & 19 and 25 & 26. Members of Group D were given toltrazuril @ 3.5 mg/kg body weight, of Group E @ 1.75 mg/kg as in group C & D, of Group F toltrazuril was given as prophylactic @ 7mg/kg on day 3 & 10. Members of Groups G & H were given irradiated (IEV) and formalized (FEV-48) Eimeria tenella vaccines respectively. IEV was orally given on day 3 & 10 whereas FEV was only given on day 10 of age.
The result indicated that maximum reduction of OPG counts (94.28 %) occurred in members of group D which was given half the dose (3.5 mg/kg) of toltrazuril, Group E (Administered quarter dose) was placed at no.2 and the reduction in this Group occurred as 93.71 %. The group G (IEV immunized) was placed at no. 3 by reducing 89.71 % mean OPG counts as compared to control Group B.
In terms of reduction of OPG counts other Groups were placed at no.4 (Group F given full dose prophylactic), no.5 (Group H immunized with FEV-48) and Group C showed the poorest performance (63.42 %) which was given full dose was placed at no. 6.
In terms of Mean body weight gains as compared to control (healthy) Group A, Group D was placed at no.1, Group E at no.2, Group G at no.3, Group C at no. 4 Group F at no.5, Group H at no.6 and Group B which acted as infected and non-medicated was placed at no.7. Ultimately, it was concluded that toltrazuril @ 3.50 mg/kg or immunization with IEV (irradiated vaccine) would be the best solution to the coccidiosis problem
Availability: Items available for loan: UVAS Library [Call number: 0941,T] (1).
