Your search returned 3 results. Subscribe to this search

Not what you expected? Check for suggestions
|
1. Isolation And Characterization Of Multidrug Resistant E. Coli From Urinary Tract Infections In A Tertiary Care

by Sumera Sabir | Dr. Aftab Ahmad Anjum | Dr | Dr. Muhammad Asad Ali.

Material type: book Book; Format: print Publisher: 2013Dissertation note: Bacterial etiology of urinary tract infections (UTIs) admitted in or visiting a tertiary care hospital in Lahore, Pakistan was determined by conventional biochemical profile. Multiple drug resistance (MDR) of Escherichia coli, the most prevalent bacteria, was checked. Overall bacterial prevalence recorded was 80.4 percent, being highest in patients of intensive care unit (93%) followed by urology ward (87%), north surgical ward (85%), east medical ward (70%) and OPD (67%). Infection rate was higher in female (87.5%) than male (71.3%) and almost same in pregnant (86%)/non-pregnant (88%) female patients. Highest percent UTIs observed were in patients of 51-75 years of age. Percent infection recorded in catheterized patients (70.8%) was lower than non-catheterized (83%) and little higher in Diabetics (82%). Out of biochemically identified bacterial isolates (n=402), highest number was of E. coli 321 (80%) followed by Staphylococcus aureus 38 (9.4%), Proteus species 22 (5.4%) and Pseudomonas species 21 (5.2%). Almost same pattern of isolation was observed among patients of different wards. On statistical analysis significantly higher number of E. coli was observed among isolates from patients of five wards included in study plan. Out of bacterial isolates from male (n=157) and female (n=245) patients highest prevalence was of E. coli (79% and 80%). Out of total bacterial isolates from female patients (n=245), number of was E. coli at the highest rank 90 (79.6%), in pregnant. Among different age groups highest prevalence was of E. coli and lowest of Pseudomonas species. Out of 120 tested urine samples collected from catheterized patients bacterial growth was observed in 85. On bacterial identification by conventional biochemical characterization highest prevalence was of E. coli (56.4%). Out of pure bacterial cultures (n=70) from Diabetic patients highest number identified was of E. coli 54 (77.1%) followed by Staphylococcus aureus 8 (11.4%), Proteus 2 (2.8%) and 6 (8.57%) were Pseudomonas species. According to Antibiotic sensitivity testing results E. coli showed highest resistance to penicillin/amoxicillin (100%) followed by cefotaxime (89.7%), ceftazidime (73.8%), Cephradin (73.8%), tetracycline (69.4%), doxycycline (66.6%), augmentin (62.6%), gentamycin (59.8%), cefuroxime (58.2%), ciprofloxacin (54.2%), Cefaclor (50%), Aztreonam (44.8%), ceftriaxone (43.3%), imipenem (43.3%), streptomycin (30%), kanamycin (19.9%), Tazocin (14%), Amikacin (12.7%) and lowest to norfloxacin (11.2%). Out of 321 E. coli 261 (81%) were declared MDR being resistant to three or more antibiotic classes. Most of the urinary tract infections in human beings are caused by E. coli which show resistance to multiple antibiotics. Availability: Items available for loan: UVAS Library [Call number: 1687,T] (1).

2. Molecularcharacterization And Antibiotic Resistance Pattern Of Pathogenic Eschericia Coli From Fancy Birds

by Nageen sardar (2011-va-401) | Dr. Muhammad Asad Ali | Prof. Dr. Aftab Ahmed Anjum | Dr.WaseemShehzad.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2017Dissertation note: Colibacilosis is a disease which is really harmful as food borne illness and many population of earth has been suffering from it. If we do early diagnosis then it would be easy to identify the disease. E. coli is an opportunistic bacteria and it can easily cause disease in favorable conditions and may also act as zoonotic issue. So the persons having birds as pets in their houses should consider for the diagnosis. So, for this purpose the more accurate tests like polymerase chain reaction should be present. E. coli can cause serious risks for poultry industry and fancy birds. Colibacillosis is caused by Escherichia coli with serious threats related to food borne illness and most of world’s population is suffering from it. Fancy birds are kept in homes as pets, zoo and safari parks for entertainment of visitors. Fancy birds may be source of transmission of pathogenic E. coli to human beings. 150 cloacal samples were taken and the process of isolation, identification, antibiotic resistance pattern and molecular characterization was done for pathogenic E. coli. 150 samples from which 50 samples were from Pet Center University of Veterinary and Animal Sciences, Lahore, 50 samples were from Lahore Jalo Park and 50 samples were from Safari Park Lahore. Samples were shifted to enrichment media and then selective and differential media. After isolation microscopic characters was observed and biochemical identification was performed. Pathogenic isolates were collected and antibiotic resistance pattern was observed. After this DNA extraction was done, extracted DNA was observed on gel electrophoresis and then PCR was performed for toxigenic genes of E. coli. Availability: Items available for loan: UVAS Library [Call number: 2824-T] (1).

3. Nephroprotective Effect Of Nifedipine Against Lead Toxicity In Mice

by Muhammad Mazhar Munir (2010-VA-179) | Dr. Muhammad Ovais Omer | Dr. Qamar Niaz | Dr. Muhammad Asad Ali.

Material type: book Book Publisher: 2017Dissertation note: This study was designed to evaluate the nephroprotective effect of nifedipine against lead toxicity in mice. Exposure to lead can induce kidney damage, which is related to induction of oxidative damage and disturbance of intracellular calcium homeostasis. Twenty mice, weighing 20.0 ±2.0 g were selected for the experiment. Twenty mice were divided randomly into four groups having five mice in every group as follows: control, lead, low-dose nifedipine and high-dose nifedipine. Mice of the low- dose and high-dose nifedipine groups were given nifedipine perorally at 10 and 20 mg/Kg/day, respectively. While the mice of the lead and control groups were administrated perorally with isovolumic saline. The mice of the low-dose nifedipine, high-dose nifedipine and lead groups were injected intraperitoneally with lead acetate 40 mg/Kg/day after treatment with nifedipine. Mice in the control group were injected intraperitoneally with isovolumic saline. The whole treatment period remains for ten days. The nephroprotective effect of Nifedipine was assessed by a decrease in histological damage to the kidneys and the concentration of lead in kidney homogenate. Moreover, the levels of creatinine and blood urea nitrogen in the serum were also determined. Data was examined as mean ± SEM (standard error mean). Data was statistically analyzed using one way analysis of variance (ANOVA). Statistical significance was considered at P < 0.05. The current study showed reduction in the concentration of lead in kidney homogenate in nifedipine treated groups compared to control positive. The lead concentration in kidney homogenate was less in the control negative group than control positive group. The level of serum creatinine and blood urea nitrogen was remarkably increased in the control positive group as compared to the control negative group. Moreover the treatment with nifedipine decreased the levels of creatinine and blood urea nitrogen in serum. Histopathological study of the kidney tissue also showed that nifedipine could improve the lead induced injury in mice It was concluded from this experiment that nifedipine has potential to minimize the lead induced nephrotoxicity in mice, as indicated by the lead concentration in kidney homogenate, serum creatinine and blood urea nitrogen levels and histopathological examination. It is, therefore recommended that nifedipine can further be investigated for nephroprotective effects against heavy metal toxicities in other animal species including livestock. Availability: Items available for loan: UVAS Library [Call number: 2863-T] (1).



Implemented and Maintained by UVAS Library.
For any Suggestions/Query Contact to library or Email:[email protected] Phone:+91 99239068
Website/OPAC best viewed in Mozilla Browser in 1366X768 Resolution.