1.
Morphological Abnormalities Of Spermatozoa In Pure Bred Friesian, Sahiwal Bulls And Friesian X Sahiwal
by Ahsan Rashad, M | Prof. Dr. Rashid Ahmad Ch | Dr. Imtiaz Hussain Khan | Dr. M. Akram | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1991Dissertation note: Of the 15 normal semen producing breeding bulls, five bulls from each of pure bred Sahiwal, Friesian and the cross-breds (Friesian X Sahiwal) of the same age group were selected. These bulls were maintained at Semen Production Unit, Qadirabad (Sahiwal) under similar conditions of housing, feeding and management. Two consecutive ejaculates once a week were collected. A total of 395 ejaculates were evaluated for various seminal characteristics, extended, processed and frozen during August, September and October, 1990. During these three months semen parameters like ejaculate volume, mass motility, percent motility and the doses produced per ejaculate declined with increase of ambient temperature and humidity.
The second ejaculate (195) at each collection and for each bull was always taken for studying the sperm morphology. Two slides from each semen sample were prepared in 10% solution of Nigrosin stain. A total of 200 sperm cells were counted in each slide and average values for various morphological abnormalities of spermatozoa in each breed were studied. The sperm cell abnormalities were accounted for head (abnormal, detached), neck (poor attachment, abaxial attachment and fractured neck), thickened mid-piece, protoplasmic droplet (proximal, distal droplet) and tail (detached, coiled- tail-droplet-enclosed, coiled tail).
The mean values of different breeds so obtained for different abnormalities during various months were analysed. The average values for head abnormalities for three breeds were 9.69 ± 1.88, 5.12 ± 060 and 1.56 ± 0.14% in Friesian, cross-bred and Sahiwal, respectively. These abnormalities were highest during August and September in Friesian bulls and lowest in Sahiwal bulls during October.
The neck abnormalities were highest in cross-breds (4.44 ± 0.57%) and lowest in Sahiwal (1.67 ± 0.14%). However, the neck abnormalities were statistically similar during the months of August, September and October. Similar trend of mid-piece abnormality was observed between various breeds and months. Likewise the values of protoplasmic droplets were highest in cross breds (14.12 ± 1.19%) and lowest in Sahiwal (2.36 ± 0.30%). The tail abnormalities were the highest in cross-breds (3.47 ± 0.45%) and lowest in Sahiwal (0.87 ± 0.12%). Among various months the tail abnormalities, were statistically similar (P> 0.05).
The total values of different sperm abnormalities wçre highest in cross-breds during August (30.93 ± 3.11%) and comparatively lower during September and October (28.66 ± 2.28 and 26.11 ± 2.63%), respectively.
Except head abnormalities, all the other abnormalities of spermatozoa were statistically significant among breeds.
Availability: Items available for loan: UVAS Library [Call number: 0206,T] (1).
2.
To Study The Comparative Efficacy Of Cloprostenol And Estradio For The Treatment Of Endometritis In Cross Bred
by Saeed Ahmad Barvi | Dr. Imtiaz Hussain Khan | Dr. Asif | Dr. Rashid Ahmad Ch | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Nature of contents: ; Literary form: Publisher: 1992Dissertation note: This study was conducted to compare the efficacy of cioprostenol and estradiol for the treatment of 1st degree endornetrët:is in cross bred dairy cows.
One hundred and sixty cross bred cows suffering from 1st degree endometrétis were selected amongst the animals brought for A.I. or repeat breeding at different A.I.Centres/Sub-centres in Lahore city. After detailed gynaecological examination animals were randomly divided into four treatment groups. Forty cross bred cows in group 'A' were injected cloprostenol on day 10 of oestrous cycle and cured animals were
inseminated at the end of induced oestrus. Similarly were in group 'B' 40 cross bred cows were injected cloprostenol on day 10 of oestrous cycle and cured animals were inseminated at the end of oestrus that followed the induced one. in group 'C' endometretic bred cows were treated with estradiol intrauterinally for 3
alternate days of oestrous cycle and the cured animals were inseminated on next oestrus, Group 'D' comprised forty cross bred cows. This group served for all other groups as control. All the animals were inseminated without any treatment. After two months all the animals were palpated rectaly for pregnancy diagnosis. Only 144 animals could he traced for pregnancy diagnosis. While others died or were sold and transferred to untraceable places. The percent curative and conception rate observed in cloprostenol treated group 'A' (ind-oestus) were 75, 66.66, respectively. Similarly in group 'B' the percent curative and conception rate in cloprostenol treated (followed lind - oestrus) aimais were 87.50, 76.47. In estradiol treated animals the curative rate was 62.50% while conception rate observed was 60.86%. In control group only 40% animals conceived after insemination. On the basis of curative and conception rates cloprostenol was found better than stilboestrol for the treatment of 1st degree endometrtis. Moreover, in cloprostenol trente(1 group resti tt of inseminat ions in Tind oestrus was found better than induced one. Therefore, cloprostenol treatment is recommended as a drug of first choice for the treatment of 1st degree endometritis in cross bred diary cows.
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3.
Studies On The Senility Pattern In Relation To Reproductive Efficincy In Male Nili Ravi Buffalo
by Capt. Farman Ahmaed Jafri | Dr. Imtiaz Hussain Khan | Dr. M. Afzal | Dr. Rashid Ahmad Ch | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 1989Dissertation note: Thirty five Nili-Ravi buffalo bulls stationed at Semen Production Unit Qadirabad were selected for the subject study. All the bulls were normal and regular donar of semen at the station. They were splitted into ten groups according to their age. Each group was carrying three to four animals. A total of 560 ejaculates were collected. Senility pattern was represented by evaluating volume, mass motility, concentration, sperm abnormality, dead/live percentage and reaction time. The data on all these parameters was collected and statistically analyzed to investigate the effect of advancing age on various seminal characteristics and reaction time in buffalo bull.
Statistically, a significant difference (P < 0.05) was noticed for all the parameters among bulls of different age groups. Maximum ejaculate volume, sperm abnormality, percentage of dead spermatozoa and reaction time were observed in age groups IX and X. Minimum ejaculate volume, percentage of dead sperms and reaction .time were observed in age group I. Mass motility and sperm concentration were maximum in age group IV while minimum in age group IX. Minimum sperm abnormality was found in age group III.
Availability: Items available for loan: UVAS Library [Call number: 1126,T] (1).
4.
Studies On Vaginal Bacterial Flora In Cyclic And Non-Cyclic Nili-Ravi Buffalo
by Sana Ullah Bhatti | Dr. Rashid Ahmad Ch | Dr. Imtiaz Hussain Khan | Dr. Muhammad | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Nature of contents: ; Literary form: Publisher: 1990Dissertation note: A total number of 60 Nili-Ravi bu±faloes were included in the study to compare the vaginal bacterial flora. Out of these 60 animals 30 were cyclic and rest of the buffaloes were non-cyclic.
Bacteria belonging to five different species were isolated from 27 out of 30 in non-cyclic and 5 out of 30 in cyclic buffaloes. From the isolates, Staphylococcus aureus had the highest incidence (16.66%) followed by Streptococcus pyogenes (13.33%), Escherichia coli (8.33%), Coryriebacterium pyogenes (6.66%) mixed infection (5%) and Bacillus cereus (3.33%). The vaginal samples of Nili-Ravi buffaloes in non-cyclic group revealed a higher bacterial count as compared to cyclic group and it was concluded that higher bacterial load in the vagina was responsible for the reproductive problems.
No strain of Brucella could be isolated. The animals treated intra-vaginally responded favourably as the viable bacterial count was lowered after the intra- vaginal treatment.
On the basis of in-vitro antibiotic sensitivity test, Strepto-penicillin was recommended for the treatment of vaginal infections as the drug of choice as it was effective against the majority of the ilates.
During the fertility trials the average conception rate was 62.93% after the treatment in the non-cyclic group.
In the light of the results of present study, a detsiled investigation would be of significant importance as far as the sexual health control measures of the buffalo are concerned.
Availability: Items available for loan: UVAS Library [Call number: 1133,T] (1).
