1.
Comparative Efficacy Of Roux-En-Y Technique With And Without Vagatomy For Pyloric Abnormalities In Dogs
by Stephen Christoper Watts | Dr.Mazhar Iqbal | Dr.Muhammad Younas Ch | Dr.Zafar Iqbal | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1992Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 0337,T] (1).
2.
Detection Of Mycobacterium Tuberculosis And Mycobacterium Bovis From Spum And Blood Samples Of Human Using a Duplex PCR
by Asma Nawaz | Prof.Dr.Zafar Iqbal Ch | Dr.Azhar | Dr.Muhammad Younas Rana | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2009Dissertation note: Tuberculosis is common infectious disease in the world. Mycobacterium tuberculosis is the most common cause of tuberculosis in the humans. Tuberculosis is endemic in Pakistan with about 1.5 million people infected. M.bovis is the major cause of gastrointestinal tuberculosis in humans.
The study was conducted in Lahore to compare 100 blood and 100 sputum samples from patients of active tuberculosis. The methods employed were conventional methods including Ziehl-Neelsen staining, culture on Lowenstein Jenson medium and biochemical tests. The Duplex PCR and conventional methods for diagnosis of tuberculosis caused by M.bovis and M.tuberculosis were compared on the sputum and blood samples. For M.tuberculosis and M.bovis the pncA gene and the species-specific 500-bp fragments were targeted in the Duplex PCR, respectively.
The sensitivity and specificity of Duplex PCR was found statistically significant in comparison to the conventional methods including Ziehl-Neelsen staining and culture for the differential diagnosis of tuberculosis caused by M.tuberculosis and M.bovis. Therefore Duplex PCR is a better choice of diagnostic test in the clinical setups where clinical urgencies necessitate a reliable, sensitive and specific test with the results in a short time period.
Availability: Items available for loan: UVAS Library [Call number: 1054,T] (1).
3.
Clinico -Pathological Studies In Cattle Suffering From Theileriosis In District Peshawar
by Dr.Akhtar Munir | Muhammad Yasin Tipu | Dr.Aftab | Dr.Muhammad Younas Rana.
Material type: ; Format:
print
Publisher: 2011Dissertation note: The present study was designed to diagnose theileriosis, to check its prevalence, to see its effects on liver and kidney function tests and to evaluate supportive therapy in the cattle population of district Peshawar. For this purpose district Peshawar was divided into 4 units and and five blood samples were collected from five villages of each unit. Thus a total of 20 villages were sampled for collection of blood samples from100 suspected animals. Screening of animals was done by blood smears stained by Giemsa staining technique. The blood smears showed theileria, piroplasms, including cocci, rod and signet-ring. On the basis of microscopic examination the overal prevalence of theileriosis in district Peshawar was 16% (16/100).
Liver and kidney function tests were perfomed on heparinized plasma using chemistry analyser (Microlab 300). The values of SGPT, SGOT, Albumin, Total bilirubin and creatinine were increased while the values of total protein and albumin were decreased as compared to the normal reference values
For evaluation of supportive therapy half of the theileria positive animals were treated with butalax only and the other half were treated with butalax + liver tonic and diuretics (Hepasel +Lasix injections)..Evaluation of supportive therapy showed that the values of liver functions tests and kidney function tests of theileria positive animals treated with Butalax + Hepasel and diuretics as supportive therapy came to the normal range after five days of treatments as compared to those treated with only butalax.
It is anticipated that present study was proved helpful in diagnosis of theileriosis and evaluation of supportive therapy and will be beneficial for further study.
Availability: Items available for loan: UVAS Library [Call number: 1243,T] (1).
4.
Effect Of Vitamin-E Supplementation On Lead Toxicity In Japanese Quail (Coturnix Japinica)
by Yaseen Humayun | Dr.Asim Aslam | Dr.Kamran | Dr.Muhammad Younas Rana.
Material type: ; Format:
print
Publisher: 2011Dissertation note: Quails are farmed in large number in Pakistan. Due to improper manage mental rearing system for quails in Pakistan the chances of lead toxicity is more in country through feed and fences and water. A total number of 420, day old chicks of Japanese quail were procured from the hatchery of Avian Research and Training Centre (ARTC), UVAS, Lahore. They were assigned seven dietary treatments. There were three replicates in each treatment group and each replicate was of twenty chicks. Control diet group A received only basal diet without any supplementation. Group B received basal diet + 50 mg/kg Pb. Group C received basal diet + 75 mg/kg Pb. Group D received basal diet +100 mg/kg Pb. Group E received basal diet+50 mg/kg Pb+40 mg/kg vit-E. Group F received basal diet+75 mg/kg Pb + 40mg/kg vit-E. Group G received basal diet+100 mg/kg Pb + 40mg/kg vit-E. The body weight of each the birds were carried out weekly and significant results were observed. Blood samples were collected on the 21st (3rd week), 28th (4th week), 35th (5th week) and 42nd (6th week) days of dietary treatment from two birds from each group to evaluate the liver, kidney functions and non significant results were observed. In liver mild degeneration of hepatocytes and increase in Kupffer cells while degeneration in the epithelium and mild fibrosis in interstitial tissue and cystic dilatations in the tubules and hyaline casts in the lumens of kidney tubules were major pathological lesions caused by lead and vitamin-E were observed.
Availability: Items available for loan: UVAS Library [Call number: 1264,T] (1).
5.
Prevalence And Biochemical Studies On Cattle Suffering From Babesiosis In District Swabi,Khyber Pukhtoonkha
by Naveed Khan | Muhammad Yasin Tipu | Dr.Habib-ur-Rehman | Dr.Muhammad Younas Rana.
Material type: ; Format:
print
Publisher: 2011Dissertation note: The present study was designed to diagnose Babesiosis in cattle in District Swabi Khyber Pukhtoonkhwa, to check its prevalence, determine its effect on the liver, kidney function tests and to check the role of supportive therapy. For this purpose blood sample was collected from 100 animals suspected to be suffering from Babesiosis in 20 different villages in district Swabi. Selection of animals was based on clinical signs like haemoglobinuria, temperature and jaundice of Babesiosis. The infection was confirmed by blood smears using Giemsa staining technique. The blood smears showed Babesia piroplasms, which were like double pear shaped or like signet-ring shaped. On the basis of microscopic examination the overall prevalence of Babesiosis in cattle in District Swabi was recorded as 10%. The blood of animals showing Babesia was analyzed in chemistry analyzer using commercially available kits.The values of SGPT, SGOT, Albumin, Total Bilirubin and Creatinine were increased while the values of Total protein an Albumin were decreased as compared to the normal reference values.
Bebesia positive animals Group A were divided into two Group B and Group C. Group B was only treated with antibabesial drug Imizol and Group C was treated with Imizol along with supportive drug i.e. liver tonic and diuretic (Hepasel+Lasix). After 5 days of treatment the plasma of treated animals was again analyzed for biochemical parameters and was compared before and after treatment (with and without supportive drug). The biochemical analysis showed that the biochemical indicators were normalizing in Group B and coming near to normal in Group C. The study is helpful in better understanding of the pathogenesis, supporting therapy and the effect of disease on the affected animals. This study showed that Babesiosis damaged the kidney and liver and the use of supportive drug along with specific drug was effective to recover the animals from infection.
Availability: Items available for loan: UVAS Library [Call number: 1267,T] (1).
6.
Effect Of Alpha Lipoic Acid On Post Thaw Quality And In Vitro Incubation Of Nili Ravi Buffalo Bull Semen
by Muhammad Hammad Fayyaz (2008-VA-171) | Dr. Mushtaq Ahmad | Dr.Muhammad Younas | Prof. Dr. Nasim Ahmad | Prof. Dr. Muhammad Sarwar Khan.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Cryopreservation is the freezing of cells or tissues to subzero temperatures, typically -196 º C. Many benefits have resulted from the process of cryopreservation. Damage induced by cryopreservation has been results cold shock, oxidative stress, osmotic changes, and formation of ice crystal and lipid–protein reorganizations within the cell membrane. Oxidative damage caused by reactive oxygen species (ROS) leads to impaired cell functions. Free radicals, includes ROS and RNS, are normal pro - oxidant molecules in aerobic metabolism. Alpha lipoic acid is a non-vitamin coenzyme that helps in significant metabolic and antioxidant functions in the body. Alpha lipoic acid has been reported to have extra functions by which they are able to synthesize vitamin C from its reduced form in the presence of glutathione. It is matchless among biological antioxidants, because it is equally lipid and water soluble. This allows it to nullify free radicals almost everywhere in the body, inside as well as outside the cells. Therefore, the objective of present study is to determine the effect alpha lipoic acid on post thaw quality and in vitro incubation of buffalo bull semen. Alpha lipoic acid scavenge on reactive oxygen species formed in semen during the process of cryopreservation, so it maintained good semen quality during post thaw and in vitro incubation. Three mature Nili-Ravi buffalo (Bubalis bubalis) bulls (4-8 year age) kept at SPU, Qadirabad Sahiwal Pakistan were used in the study. These bulls are being used as regular donors at SPU. There semen was collected with artificial vagina of temperature 42c; three ejaculates (one from each) was pooled and diluted (30 million sperms/ml) with extender of different inclusion levels (0.0, 0.5, 1, 1.5, 2, 2.5 mmol/ml) of alpha lipoic acid. Straws were filled and extended then semen was cooled for 2 hours and equilibrated for two hours. Semen was placed in Liquid nitrogen vapors for 10 minutes. Finally semen straws was put in liquid nitrogen, Total five replicates were performed. Now post thaw quality was checked in
Summary
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which various tests were performed, like %age motility, Acridine orange assay for DNA integrity, HOST for plasma membrane integrity, Fitc-PNA/PI for viability and acrosomal integrity. Longevity test was performed by in vitro incubation of frozen thawed semen sample in SOF and evaluating it at 1.5, 3 and 4.5 hour interval in Carbon dioxide incubator. It was expected that Alpha lipoic acid shown positive effect on post thaw quality and in vitro incubation of buffalo bull semen, in the meaning of increased percentage motility, Less DNA damage during cryopreservation and incubation, Increased acrosomal and plasma membrane integrity. So alpha lipoic acid shown positive effect by counter acting on ROS during cryopreservation and in vitro incubation. Results acquired from this study shown that an increase in sperm motility, plasma membrane integrity, DNA integrity, Acrosomal integrity, viability and survival was caused by ALA competences in energy production and anti-oxidant properties, when used at the concentration of 0.5mM and 1mM. In summary, based on the results of our study, it can be concluded that an optimal concentration (0.5mM and 1mM) of ALA improved PMI, sperm motility and viability, minimize DNA damage and improved sperm survival. Availability: Items available for loan: UVAS Library [Call number: 2268-T] (1).
