6.1 Background
The proper disease diagnosis in large ruminants is required for overcoming the economy loss due to livestock poor management. Blood biochemical and hematological parameters are one of an important medium to assess the physiological and pathological condition of animals. Any physiological and pathological change can be evaluated only if the normal values are available for comparison. Factors such as age, breed, geographical location, diet, sex, diurnal variation, physical activity, pregnancy and environment are known to influence the serum biochemistry and haematology of large ruminants. Our hypothesis was to evaluate the effect of different physiological stages (pre-pubertal and pubertal) on hematological and serum biochemical parameters of Nili-Ravi buffalo and Sahiwal cattle.
6.2 Materials and Methods
Sixty blood samples (30 Cattle and 30 Buffalo) were collected from Ravi Campus Pattoki, UVAS and divided on the basis of different physiological stages, pre-pubertal (10-12) and pubertal (22-24 months) into two groups. The collected samples used to estimate complete blood counts by hematological analyzer. The biochemical parameters include cholesterol, triglycerides, total protein, urea, AST and ALT by commercially available kits (Biogene and crescent).

6.3 Statistical Analysis
Statistical Package for Social Science (SPSS) version 17 used. Further T Test was used to compare the hematological and blood biochemical between two age groups. Differences was considered significant at P<0.05 (Steel et al.1997).
6.4 Results
The hematological parameters RBCs, Hb and PCV were showed a significant difference at Pre-pubertal and pubertal stage of both buffalo and cattle. Whereas the WBCs count, lymphocytes, granulocytes, monocytes, MCV and MCH were not found significantly different at pre-pubertal and pubertal stages of buffalo and cattle. The serum level of glucose recorded highest and urea level lowest at pubertal stage in both buffalo and cattle. The cholesterol and triglyceride were found significantly (p<0.05) lowest, while liver enzyme AST and ALT were found significantly highest with the aging of animal in both species. No significant changes were observed in total protein level at pre-pubertal and pubertal stage in buffalo and cattle.
6.5 Conclusions
The WBCs, RBCs, Hb, PCV were found significantly different between pre-pubertal and pubertal stages in both species. The values of theses parameters recorded highest at pubertal stage, while granulocytes, lymphocytes, monocytes, MCV and MCH were found non-significantly different. Different physiological stages had a significant effect on glucose, cholesterol, triglycerides, Urea, ALT and AST in both species. The results of this study indicated that different physiological stages must be taken as very important factor in determining the blood parameters.