1.
A Study On Prevalance Of Hcv Genotypes And Risk Factors Of Hepatitis C Virus In Punjab
by Tahira Tarar | Miss Faiza Masood | Dr. Muhammad | Mr. Zahid Mushtaq.
Material type: ; Format:
print
Publisher: 2011Dissertation note: Hepatitis C virus (HCV) was marked as major agent which causes non-A, non-B hepatitis. Various partial and complete sequences of HCV nucleotide had been identified in the world. When these sequences were compared a marked genetic heterogeneity was revealed, that suggested the existence of HCV genotypes. Recent studies have pointed out the association of different HCV genotypes with different profiles of pathogenecity, infectivity and response to antiviral therapy. In our study we used typing system based on genotype specific primers focused on HCV 5´-UTR by using PCR. Genotype specific primers were designed for genotype 1, 2a, 2b, 3a, 3b and 4. 100 samples of HCV positive patients were collected. The frequency of occurrence of genotype 2a was 4 %, 2b was 5 %, 3a was 71 %, 3b was 11% and untypeable was 9 %. Blood transfusions, therapeutic injections, reuse of needles, dental procedures, shaving practices, body piercings, jaundice, dialysis, surgery and other multiple risk factors associated with HCV were studied. Major risk factor among females was therapeutic injections and among males the major risk factor was absence of shaving precautions. The study concludes that the most prevalent genotype in Punjab province is 3a. As each genotype sequence is different, the antiviral therapy against that particular genotype is also different. The treatment would only be successful if the genotype of HCV infected patient is known. This study will help in correlating efficacy of interferon therapy with different HCV genotypes and to understand the mode of transmission for hepatitis C virus.
Availability: Items available for loan: UVAS Library [Call number: 1336,T] (1).
2.
Preparation Of Turnip Peroxidases And Its Application To Remove The Phenolic Content Of Sannerty Effluent
by Muhammad Usman Amin | Dr. Abu Saeed Hashmi | Miss. Faiza Masood | Mr. Tanveer.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2011Dissertation note: Peroxidases are heme-containing oxidizing enzymes, which are wide spread in nature. They have the ability to catalyze the oxidation of many organic and inorganic electron donor substrates through a reaction with hydrogen peroxide or organic hydrogen peroxides. In this study peroxidase were purified from turnip using ammonium sulphate precipitation, poly ethylene glycol precipitation and zinc sulphate precipitations in order to find some simple and less expensive procedure for partial purification of peroxidases. Ammonium sulphate and PEG (6000) in the presence and absence of NaCl were used to make aqueous two phase system. Aqueous two-phase system (ATPS) without NaCl purified enzyme most efficiently. (NH4)2SO4 layer was subjected to dialysis and for further purification on sephadex gel which gave maximum enzyme activity of 1544u/mg protein. SD-PAGE analysis was done to determine enzyme purity. Purified enzyme was charged into the tannery waste water along with H2O2 to remove toxic phenolic content up to 98.24%.
Availability: Items available for loan: UVAS Library [Call number: 1356,T] (1).
3.
To Study The Extraction, Purification And Characterization Of Papain Form Carica Papaya
by Hafiz Anis-Ur-Rehman Tariq | Miss Faiza Masood | Dr. Abu Saeed Hadhmi | Mir.
Material type: ; Format:
print
; Nature of contents: ; Literary form: Publisher: 2011Dissertation note: In this piece of research work, the Extraction, Purification and Characterization of Papain (a proteolytic enzyme) from Papaya fruit (Carica Papaya L. of Caricaceae family) were carried out. For this purpose, the Peelings, Flesh and Seeds of the Papaya fruit were used. Extraction of Papain was done using 0.1M alkaline Phosphate buffer. Purification of Papain was carried out by Ammonium Sulphate precipitation and dialysis followed by Gel filtration by Sephadex G-75. Then Characterization of Papain such as protein estimation, determination of proteolytic activity (International Unit) of enzyme and SDS-PAGE analysis were performed to determine the molecular weight. Finally, the yield and proteolytic activity of the Papain obtained from the Peelings, Flesh and Seeds of the Papaya fruit was measured and compared with the commercial product available in the market.
Availability: Items available for loan: UVAS Library [Call number: 1362,T] (1).
4.
Production, Purification And Characterization Of Xylanase Enzyme From Mutant Aspergillus Flavus Strain
by Rukhsana Tahir | Miss. Faiza Masood | Dr. Abu Saeed Hashmi | Dr. Aftab.
Material type: ; Format:
print
Publisher: 2012Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1506,T] (1).
5.
Production Of Synbiotic Product Containing Galacto-Oligosaccharides And Saccharomyces Boulardii And Evaluation Of Its In Vitro Bifidogenic Effect
by Farah Javed (2013-Mphil-1110) | Dr. Sanaullah Iqbal | Dr. Zubair Farooq | Miss Faiza Masood.
Material type: ; Literary form:
not fiction
Publisher: 2015Dissertation note: Food are not only used to satisfy hunger or provide nutritional support but also used to prevent diseases that are based on nutrition and thus improve the health of consumers (Menrad, 2003).A food that is same in appearance and consumption to conventional food but has physiological benefits and prevents diseases is known as functional food (Sangwan et al. 2011). Functional foods are used to provide benefit to intestinal microflora. The most important functional food are prebiotic and probiotic. The combination of prebiotic and probiotic are known as synbiotics. The symbiotic is effective as that of prebiotic and probiotic. The probiotic is used to reduce the impurities from prebiotic mixture. This is very important to know the functional property of functional food (Sangwan et al. 2014) that is galactooligosaccharides (GOS) in present research.
GOS are most effective prebiotic which are beneficial to intestinal microbiota. Other health effects are stool improvement, mineral absorption, weight management and also improve the growth of Bifidobacterium and Lactobacillus (Macfarlane et al. 2008). The Saccharomyces boulardii is a non-pathogenic yeast and used as probiotic. It is thermo tolerant yeast and optimally grows at body temperature i.e; 37ºC. Due to this property S. boulardii can be used for the treatment of gastrointestinal diseases. Another function of this yeast is the reduction of monosaccharides from prebiotic mixture.
The purposes of present research work are to reduce the monosaccharides form mixture of GOS and the production of synbiotic product containing GOS and S. boulardii. For this GOS
were produced through transgalactosylation procedure using lactose as substrate. S. boulardii was grown separately and incubated with the mixture of GOS. After that symbiotic product was formed and lyophilized it. The total count of yeast was determined in lyophilized mixture. The in vitro bifidogenic effect of GOS and lyophilized product was also checked.
Availability: Items available for loan: UVAS Library [Call number: 2291-T] (1).
6.
Conversion Of Pretreated Rice Husk Hydrolysates Into Ethanol By Saccharomyces Cerevisiae
by Jehanzaib (2014-VA-08) | Dr. Rahat Naseer | Miss Faiza Masood | Prof. Dr. Saima Naveed.
Material type: ; Literary form:
not fiction
Publisher: 2016Dissertation note: Biodegradable wastes wereresidues of commercial crops.Agricultural wastes such as rice husk was utilized for biofuel production such as ethanol by breaking downinto its components using chemicals or enzymes. This was achievedthrough anaerobic digestion, distillation and fermentation for use as a resource of heat and fuel (bioethanol).Bioethanol meansan alternative to petrol which is produced from plants such as sugar cane or maize and rice husks.Agricultural wastes can be utilized for the biofuel, rice husk often pose.Acid and alkali pretreatment was enhanced the yield of ethanol from rice husk.Rice husk wastreated with alkali and acids to produce glucose. Glucose can be estimated byusing glucose oxidase method (GOD). Glucose was then converted into ethanol using Saccharomyces cerevisiae. Ethanol production was estimated by using spectrophotometer.The purposed project wasfocusing on bioethanol from the rice husk residues in which rice husk was pre-treated for the hydrolysis to produced maximum yield of sugar by selection of best pretreated method. And then sugars were converted into bioethanol by fermentation. Alkaline solutionswere remove lignin, while the acidic solutionswere remove hemicellulose. It was clearly showed in the graph.4.1 and 4.2 that best concentration of alkali 4% for the maximum yield of sugar was 50.4 mg/dl from the maximum 15g substrate of rice husk was used at 70˚C for 3hrs while in case of 4% acid treatment was also best for the maximum 42.6mg/dl sugar yield was obtainedat 70˚C for 3hrs.
According to statistical analysis and graphs 4.3 and 4.4 showed that maximum yieldi.e, 68.7 ml ethanolwas produced from the 15g rice husk when treated with 4%alkali,maximum temperature 35˚C with PH 6.0 and after 3days.While in case of 4%acid treatmentmaximum 63.7 ml ethanol was producedwhen maximum 15g rice husk was used at temperature 35˚C andPH with 6.0.after 3days.
Availability: Items available for loan: UVAS Library [Call number: 2644-T] (1).
