1.
Evaluation Of Canola Meal & Its Feeding Effects On The Performance Of 0-25 Weeks Old Layer Chickens
by Rafiq, M | Prof.Dr.Talat Naseer Pasha | Prof.Dr.Muham | Prof.Dr.Nisar Ahmad Mian.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1999Dissertation note: Canola meal is the by-product of the oil industry, available after extraction of oil from the seeds by solvent extraction. After the animal protein sources, canola meal is among the best protein supplement which could be incorporated in different poultry feeds.
The present experiment was conducted to study the effect of different levels of canola meal on the perfromance of layer chicks (216) from 0-25 weeks. The chicks were devided into six main groups, having 36 chicks each and were sub-devided into 3 replicates of 12 chicks each. Each replicat was reared as an experimental unit. All the managemental conditions like lighting, feeding, watering, beek trimming, ventilation, vaccination and temperature were provided according to the recommend requirements for Babcoock B300 layer chicks strain.
Six experimental rations containing 0,9, 12, 15, 18 and 21% canola meal in starter and grower rations while in layer mash 0, 5, 7.5, 10, 12.5 and 15%. These rations formulated and provided according to the Babcoock B300 strain requirements. In the study, effect of feeding different levels of canola meal was checked in lieu of soybean meal on the feed intake, weight gain, feed efficiency, sexual maturity, uniformity, percent egg production, kilograms of feed per dozen eggs, kilograms of feed per kilogram egg mass, weight of 12 eggs, mortality and economics. In starting and growing phase weight gain and feed efficiency was non significant while feed consumption was significant (P < 0.01) in growing and laying phases. Egg mass, Percent egg production, egg weight, weight per 12 egg, feed efficiency (kilograms of feed per dozen eggs, kilograms of feed per kilogram egg mass) showed nonsignificant difference. Mortality was also non-significant among the vnrious experimental groups. Economics of the experimental rations indicated that rations contain canola meal were cheeper as compared to soybean meal containing rations. The overall picture of the whole experiment revealed that canola meal can replace soybean meal in starting, growing and laying phases up to 100% but it has little problem of poor feed intake which was due to picking problem of the birds because the partical size of canola meal is small.
Availability: Items available for loan: UVAS Library [Call number: 0613,T] (1).
2.
Prevalence & Chemotherapy Of Ascariasis & Its Effect On Various Blood Parameters In Horses
by Saqlain Shah Bukhari | Dr.Khalid Pervez | Dr.Muhammad Sarwar Khan | Prof.Dr.Muham | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 1999Dissertation note: (Albendazole) was 64.95%, 52.26% and 44.02% on 7th, 14th and 21st day, respectively. Efficacy of Oxafax (Oxfendazole) was 99.18%, 98.36% and 97.95% on 7th, 14th and 21st day, respectively. Efficacy of Ivomec (Ivermectin) was 100%, 10O% and 100% on 7th, 14th and 21st day, respectively. It was concluded from above results that Ivermectin was found the most effective than Albendazole and Oxfendazole, while Oxfendazole showed better efficacy than Albendazole.
Haematological parameters showed reduction in total erythrocyte count (TEC), haemoglobin (Hb) level and packed cell volume (PCV).
Availability: Items available for loan: UVAS Library [Call number: 0629,T] (1).
3.
Study Of Changes In Hematological Laboratory Profile Induced By Vinorelbine In Cancer Patients
by Taha Nazir | Prof.Dr.Saghir Ahmad Jafri | Dr.Muhammad Mehmood Khan | Prof.Dr.Muham.
Material type: ; Format:
print
Publisher: 2000Dissertation note: The present study was carried to investigate the hematological alterations in the patients diagnosed as cancer and were administered vinorelbine as part of their chemotherapy at Shoukat khanum memorial cancer hospital and research centre. It was carried in the chemotherapy department, pathology laboratory, medical record section, pharmacy department, and data processing department of the hospital. For investigating there were a total of 60 adult cancer patients with non-small cell lung cancer (NSCL), metastatic breast cancer (MBC), and cancer of cervix (CC), with age between 24 to 71 years (Mean±SEM: 42.73±2.69) selected. Which were divided into two groups: Group - I for those patients who were on the treatment protocol of Vinorelbine alone (25 mg/m2/day one, weekly x 4) and group - II for those patients who were on treatment protocol of Vinorelbine base combinations Vinorelbine/Doxorubicin (Vinorelbine - 20 mg/m2/day one and day eight, Doxorubicin - 50 mg/m2/day one only) and Vinorelbine/Cisplatin (Vinorelbine - 20 mg/m2/day one and day eight, Cisplatin - 40 mg/m2/day one only). The Laboratory tests (C.B.C.) of all the patients were performed in the Pathology Laboratory of the hospital. After completion of the study, the collected Hematological values were statistically analyzed by student pair test. By the overall comparison of mean values over time, there were significant leukopenia (neutrophile, eosinophil, & monocyte), anemia and decreased hematocrit value observed in the patients on chemotherapy protocol of Vinorelbine alone and significant leukopenia (neutrophile only), anemia and decreased hematocrit value and hemoglobine conc. observed in the patients on chemotherapy protocol of Vinorelbine based combinations. By an independent comparison of mean values of two groups at every week, there were significantly higher toxicities 1 e leukopenia at week-3 (neutrohpil at week-3, eosinophil at weekI, lymphocyte at week-i &2) and thrombocytopenia at week-i observed in the patients on the treatment protocol of Vinorelbine based combinations. When the mean values observed before therapy were compared with that of at week-4 of both of the groups, there were significant decreases noted in TLC, neutrophil count, monocyte count, erythrocyte count, hemotocrit value and Hb conc in the patients on treatment protocol of Vinorelbine alone, and erythrocyte count, hemotocrit value and hemoglobine conc. in the patients on treatment protocol of Vinorelbine based combinations. It is concluded from this study that, there is insignificant difference in the overall hematological toxicities produced by both of the chemotherapy protocols. The clinical oncologist, consultant physicians and pharmacist, therefore now suggested to select the either of the of the chemotherapy protocol with respect of the therapeutic efficacy only
Availability: Items available for loan: UVAS Library [Call number: 0661,T] (1).
4.
Pathogenesis Of Salmonellosis With Respect To Carrier States In Poultry And Its Public Health Impact
by Younus, M | Prof. Dr. Zafar Iqbal Chaudhry | Prof.Dr.Abdul Rauf Shakoori | Prof.Dr.Muham | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2006Dissertation note: The present research endevour was made to study and investigate the prevalence of Salmonella enteritidis and Salmonella typhimurium from poultry feed, poultry meat and poultry eggs and their role in the chain of transmission of salmonellae to human beings. The objective was to generate data to improve the quality of poultry products and human health awareness.
Salmonellosis is one of the most wide spread food borne zoonoses. The etiological agents Salmonella enteritidis and Salmonella typhimurium not only' produce the disease but during the convalescent phase (after the recovery of disease) remain carriers for indefinite period of time. In this study 400 samples were collected and were distributed and detailed as; poultry feed (n=100), poultry intestines (n100 Small and n=100 Large intestines) and eggs (n=100) were collected for the identification of the organism through polymerase chain reaction (PCR). The Positivity percentage as tested through PCR for Salmonella enteritidis in the poultry feed was 20,15,10,15 and 10 for layer starter, layer grower, layer finisher, broiler starter and broiler finisher respectively (P>0.05). The positivity percentage as tested through PCR for Salmonella typhimurium for layer starter, layer grower, layer finisher, broiler starter and broiler finisher feed was 15,10,10, 10, and 10 respectively (P>0.05). There was no significant difference between layers feed and broilers feed as far as identification of salmonella enteritidis and salmonella typhimurium was concerned (P>0.05) but the prevalence range of salmonella enteritidis and salmonella typhimuilum from poultry feed was 10-20% which was biologically significant. The positivity percentage rate of Salmonella enteritidis for small and large intestine in Desi birds (local breed) was 2 and 16 % respectively. Where as for broilers in small and large intestine it was 4 and 18% respectively. The positivity of Salmonella typhimurium in small and large intestine of Desi birds was 2 and 14% where as in broilers it was 4 and 16% in the small and large intestine respectively. There was a significant difference (P <0.05) between the positivity of percentage of salmonella enteritidis and salmonella typhimurium as far as identification of Salmonellae from Desi and broiler meat was concerned.
It was found that 16%, 8%, 16'Y0 and 16% egg albumin was found positive for Salmonella enteritidis in layer egg albumin, Desi (local breed) eggj albumin, double yolk albumin and broken egg albumin respectively. In each case 25 egg albumin were collected and tested for the detection of Salmonellae. Similarly the egg yolk from layers, Desi (local breed) double yolk and broken eggs was taken and positivity rate for Salmonella enteritidis was found 12%, 4%, 12% and 12% respectively. It was found that 12%, 4%, 12% and 12% egg albumin was found positive for Salmonella lyphimurium in layer egg albumin, Desi egg albumin, double yolk albumin and broken egg albumin respectively. In each case 25 egg albumin were collected and tested for the' detection of Salmonella. Similarly the egg yolk from layers, desi double yolk and broken eggs was taken and positivity rate for Salmonella enteritidis was found 8%, 4%, 8% and 4% respectively. The positively rate for Salmonella typhimurium in both albumin and yolk was relatively less in both albumin and yolk of layers, desi double yolk and broken eggs. Statistically there was no significant difference (P> 0.05) but the prevalence of Salmonella enteritidis and Salmonella typhimurium from different eggs ranged between 4-16% and 4-12% respectively which was biologically significant.
The Salmonella enteritidis and Salmonella typhimurium were isolated, identified and grown on the artificial and selective media. The virulence of the organisms of Salmonella enteritidis and Salmonella typhimurium were estimated through calculation of LD50. It was found as 10358/mI and 103/ml for Salmonella enteritidis and Salmonella typhimurium respectively, having significant difference (P< 0.05). In order to understand the pathogenesis and carrier states of salmonella organisms in poultry, a group of 300 broiler birds were procured and divided into three groups were studied upto the age of 3 months. The infection was orally given on the 7th day of their age. As an average 86.74% of the birds were maintaining the organism of the Salmonella enteritidis in the large intestine during the entire experimental period in contrast to the small intestine in which 0% were found positive (P< 0.05). Similarly an average 94.94% of the birds were maintaining the organism of the Salmonella typhimurium in the large intestine during the entire experimental period in contrast to the small intestine in which 0% were found positive (P< 0.05) but non of the samples of Small and Large intestine of control group (Group-C) were found positive for Salmonella enleritidis and Salmonella typhimurium. There was a significant difference between Salmonella enteritidis and Salmonella typhimurium in large intestine of poultry (P< 0.05). The histopathology of different organs of broiler chickens i.e liver, lung, spleen, kidney, small intestine, large intestine, bursa of fabracious and lean muscles at different phases of disease was also conducted for the better understanding of pathogenesis due to salmonellosis. The principal lesions in the liver at the age of 14 to 28 days in groups A and B were leukocytic infiltration, necrosis and haemmorrhage. No lesions were recorded in liver after 28 days of age in groups A and B. No lesions were recorded in group C. The principal lesions of the lungs at the age of 14 to 28 days in groups A and B were leukocytic infiltration,' mild necrosis, vascular congestion and haemrnorrhages. No lesions were recorded in lungs after 28 days of age in groups A and B. No lesions were recorded in group C. The principal lesions of the spleen were mild leukocytic infiltration, necrosis and congestion at the age of 14 to 28 days in groups A and B. No lesions were recorded in spleen after 28 days of age in groups A and B. No lesions were found in group C. The principal lesions of the kidneys were marked tubutar necrosis with glomerular degeneration and Ieukocytic infiltration and haemmorrhages at the age of 14 to 28 in groups A and B. No lesions were1 recorded in kidneys after 28 days of age in groups A and B. No lesions were found in group C. The principal lesions of the small intestine were degeneration of mucosa with inflammatory cells, necrosis, inflammation, superficial ulceration on mucosal lining of intestine at the age of 14 to 21 days. No lesions were recorded in small intestine after 21 days of age in group A and B. No lesions were recorded in control group C. The principal lesions of the large intestine were leukocytic infiltration with necrosis and inflammation at the age of 14 to 91 days. The lesions were recorded up to 91 days of age in group A and B. No lesions were recorded in control group C. The principal lesions of Bursa of1, fabricious were atrophy & necrosis of bursal follicles and leukocytic infiltration at the age of 14 to 21 in groups A and B. No lesions were recorded in Bursa of fabricious after 21 days of age in groups A and B. No lesions were found in group C. The principal lesions of lean muscle were muscular degeneration and necrotic areas at the age of 14 to 21 days in groups A and B. No lesions were recorded in lean muscles after 21 days of age in groups A and B. No lesions were found in group C.
The carrier state was not only the source of spread of disease with in the poultry but also caused typhoid fever and food poisoning in humans. The chain of transmission started fron poultry feed to poultry meat and ultimately to humans as dead end host. Finally, the 400 samples of stool and blood from 200 human patients (100 suspected of typhoid fever and 100 suspected of food poisoning) were also collected from four different hospitals from urban area of Lahore for the identification of Salmonella enteritidis and Salmonella typhimurium through PCR method in order to see the public health impact of Salmonellosis through consuming the meat and eggs of the carrier birds. A total of 14% and 10% stool samples were found positive for Salmonella enteritidis and Salmonella Typhimurium in case of suspected typhoid fever patients respectively. Similarly 6% and 2% blood samples were found positive for Salmonella enteritidis and Salmonella Typhimurium. There was a significant difference (P< 0.05) in the sero positivity of stool and blood samples of suspected typhoid fever patients and also as for as Salmonella enteritidis and Salmonella typhimurium was concerned. However there was no significant difference (P> 0.05) between the hospitals On the average 14 and 10 stool samples were found positive against Salmonella enteritidis and Salmonella typhimurium from each of the 25 patients of each hospital respectively in case of suspected food poisoning patients. Similarly on an average 5% and 6% blood samples were found positive from 25 patients of each hospital respectively. There was a significant difference (P< 0.05) in the sero positivity of stool and blood samples of suspected food poisoning patients as far as Salmonella enteritidis and Salmonella typhimurium was concerned. However there was no significant difference (P> 0.05) between the hospitals.
CONCLUSION
A series of five experiments were conducted and carried out to study and explore the project Pathogenesis of Salmonellosis with respect to carrier states in poultry and its public health impact."
For this purpose, in the 1st phase, identification, isolation and characterization of Salmonella enteritidis and Salmonella typhimurium was attempted. It was followed by the estimation of LD 50 and carrier states and histopathological study at different phases of disease in broiler chickens experimentally infected with Salmonella enteritidis and Salmonella typhimurium to ascertain the nature of carrier states in terms of maintenance of the Salmonellae by different organs leading to histopathological changes and finally to the stage of shedding of the organism through the feces in the environment. Dissemination to human beings and the Public health impact of Salmonellosis was studied in the human subjects who consumed the meat and eggs of the carrier birds which were followed by testing their stool and blood samples through polymerase chain reaction (PCR). In this way the pathogenesis and chain of Salmonellas enteritidis and Salmonella typhimurium infection through poultry feed, meat, eggs and humans beings was transmissible. However, the humans were considered as dead end host. It was concluded that Salmonella enteritidis and Salmonella typhimurium was maintained in the large intestine of the poultry and has transmitted from poultry feed, poultry meat and poultry eggs to human beings and thus, causing typhoid fever and food poisoning.
RECOMMENDATIONS /SUGGESTIONS
Major aim of this research endeavour was to help in understanding the basic principles involved in the chain of infectious cycle of SalmoneUosis. In addition to that the application of the quality control of poultry products with respect to Salmonella infection to broiler chicks and broiler meat available in the market for human consumption is the ultimate goal of this project. The objective was to reduce the risk of Salmonellosis in poultry and humans. The following measures are suggested.
1. PREVENTION AND CONTROL OF SALMONELLOSIS IN POULTRY! ANIMALS
A. Monitoring
o The poultry and their environment should be monitored by frequente testing of Salmonellae.
o Bacteriological profile of poultry house environment.
o Serological testing of flock and removal of infected birds.
o Culturing of tissues from selected birds.
o Egg sheils, egg albumin & egg yolk culturing.
B. Hygiene and Sanitation
o Eggs from infected layer flocks should be pasteurized before consumption.
o Salmonella positive breeder flocks should be given pellet feed.
o Hatching sanitation
o Proper disinfection of hatching eggs.
o Proper sanitation and disinfection of farm premises.
o The provision of salmonella-free feed i.e pellet feed is of prime importance for the prevention of salmonella infections of poultry flocks and parent flocks.
o Control of rodent, insects and wild birds
C. Managemental
o For routine treatment of eggs and progeny, only those antibiotics should be used that do not cause microbial resistance against drugs widely used in humans
o Resistance of Campylobacter spp, and Salmonella spp. to fluoroquinolones has become a public health risk. This does not exclude well targeted and transient use of antibiotics as essential measures in salmonellosis control programmes.
o Vaccination of breeder flock is recommended for decrease of the salmonella infection pressure.
7
1. MEASURES FOR THE PREVENTION AND CONTROL OF SALMONELLOSIS IN HUMANS
A. Meat and Eggs
o Wrap fresh meat in plastic bags at the market to prevent blood from1 dripping on other foods.
o Cook poultry products at temperature of 170°F for breast meat and at 180°F for thigh meat.
o Avoid eating raw or under cooked meat and egg.
o Cook poultry meat and egg thoroughly.
o Purchase only inspected grade AA eggs and animal food products.
o Handle raw eggs carefully:
o Keep eggs refrigerated
o Throw away cracked or dirty eggs.
o Do not eat half fried and half boiled eggs.
o Wash hands immediately after handling raw poultry or raw eggs.
o Full fried and full boiled eggs should be used for eating to prevent food borne Salmonellosis problem.
b. PERSONNEL HYGIENE MEASURES
o Washing of hands with soap and warm water before and after handling foods, after using the bath rooms.
o Refrigerate foods properly.
- Use bleach to wash cutting boards and counters used for preparation immediately after use to avoid cross contamination of other foods.
o People who have Salmonellosis should not prepare food for others.
o Educate the food handlers and persons who prepare food. Educational programmes covering pre- and post harvest food safety procedures, especially salmonella control, should be initiated in the animal and food production sectors for the public awareness.
Availability: Items available for loan: UVAS Library [Call number: 0938,T] (1).
5.
A Study Of The Theileriosis Sheep At Livestock Production Research Institute Bahadurnagar District Okara
by Zia Ur-Rehman | Prof.Dr.Muhammas Sarwar Khan | Mr.Muhammad Avais | Prof.Dr.Muham | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2008Dissertation note: In the present course of research 400 sheep from Livestock Production Research Institute, Bahadurnagar Okara had been examined for the evidence of theileriosis by using Giemsa stain blood smear, lymph node smear methods and by clinical signs. Out of 400 animals 66 were found positive for theileriosis by observing blood smears with giemsa stain. The over all infection rate was 16.5%, while 43 sheep were exhibiting the clinical signs of malignant ovine theileriosis which was 65.15% Of total infected sheep. All the animals which were clinically positive were also lymph node smear positive. Distribution of infection among different age groups was observed as 17.3% in lambs and 16.20% in adults while distribution among sexes was as 15.8% in males and 16.6% in females.
The clinical trials were conducted on positive sheep by treating them with oxytetracycline and buparvaquone. In this part of experiment 30 positive and 10 negative sheep were placed in four groups with 10 animals in each group. Ten positive sheep were kept in group A and were treated with Rimoxyn and 10 other positive animal were placed in group B in which Butalex was used against ovine theileriosis. Group C also comprises of 10 positive sheep and serve as positive control while 10 sheep of group D which were found negative against theileriosis served as negative control. The drug used in group A i.e., Rimoxyn show 30% efficacy, 3 animals recover on up to 7th day and no more found recovered on day 10. In group B Butalex was used which showed 90% efficacy in the whole study, 7 out of 10 recovered up to day 7 and 2 more positive sheep recovered at 0 day.
Blood parameters (Hemoglobin estimation, Total Leukocytic count TLC Differential leukocytic count DLC) were studied to observe the effect of protozoa and use of different drugs on various blood parameters. Variable results were observed in different blood parameters. In groups A and B significant difference (P<0.0001) between hemoglobin level of day 0, 7 and 10 was observed, while there was insignificant difference in the values of Total Leukocytic count (TLC) at day 0, 7 and 10. In DLC, insignificant difference observed in the percentage values of neutrophils, monocytes and basophiles while significant differences were in percentage values of lymphocytes and eosinophils both in group A and B. The animals of group C were kept as infected/positive control. Statistical analysis showed that there was insignificant difference between hemoglobin level of day 0, 7 and 10, results of Total Leukocytic count TLC were also found insignificant. In Differential leukocytic count DLC, insignificant difference was observed in the percentage values of all five types of leukocytes i.e., neutrophils, lymphocytes, monocytes, eosinophils and basophiles. The hematological values of group D were also found insignificant, animals of this group were kept as healthy control.
With the results of present study, it has been concluded that buparaquone (Butalex) was the drug of choice for the treatment of ovine theileriosis in Lohi sheep when given at the dose rate of 2.5mg/kg body weight by intramuscular route. It has been further observed that there is a need of further research in the diagnosis and treatment of malignant ovine theileriosis.
Availability: Items available for loan: UVAS Library [Call number: 0981,T] (1).
6.
Detection And Chemotherapy Of Sub Clinical Mastitis In Dairy Catlle And Buffaloes
by Muhammad Jamil | Dr.Jawaria Ali Khan | Dr.Muhammad Iqbal | Prof.Dr.Muham | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2008Dissertation note: The aim of this study was to detect subclinical mastitis in bovine and to find out the most appropriate antibiotic for its treatment. For this purpose, Milk samples were taken aseptically from 500 apparently healthy animals (n= 250 cattle and n= 250 buffaloes) for screening tests. Two different tests, Surf Mastitis Test (SMT) and Somatic Cell Count (SCC) were used. Milk samples declared positive by both of the above mentioned tests, were subjected to culture sensitivity test. Six different antibiotics were evaluated i.e. Enrofloxacin, Norfioxacin, Amoxicillin, Oxytetracycline, Gentamicin and penicillin G. On the basis of sensitivity test; two topmost drugs were selected and be given to two equal groups of animals. Each group was comprised of equal number of cattle and buffaloes. Each antibiotic was given for 5 days as intramuscular (IM) injection. A positive and a negative control groups were also kept. To detect the in-vivo efficacy of antibiotic, again the milk samples of all the groups were examined by SMT and SCC on day 10 and day 20 of first injection. The results obtained were the following;
Sub clinical mastitis was found 42.8% in cattle and 37.6% in buffaloes. Out of
the total of 201 mastitis positive milk samples cultured, bacterial growth occurred in
98 (48.75%) of milk samples. The various bacterial species isolated from milk
samples of cattle and buffaloes were E. coli Staphylococcus, Streptococcus, Proteus,
Kiebsiella, Pseudomonas, and Pasteurella with overall percentage of 50 %, 17.34 %,
3.06 %, 12.24%, 10.20%, 2.04% and 5.10 % respectively.
The in vitro efficacy of Enrofloxacin was found to be the best one i.e. 77.55 % followed by Norfioxacin (67.34 %), Gentamicin (53.06 %), Oxytetracyclin (30.61 %), Amoxicillin (22.44 %) and Penicillin G (4.08 %).
After chemotherapy, there was significant difference between treatment groups and control groups (P<0.05). The difference in the efficacy of enrofloxacin and norfioxacin was statistically non significant (P>0.05), however mathematically, the recovery rate by enrofloxacin was greater (93.75%) then norfioxacin (87.50%). Recovery rate was more at day 20 for both antibiotics.
Availability: Items available for loan: UVAS Library [Call number: 1008,T] (1).
7.
A Study On Mastitis In Pregnant And Non Pregnant Cattle And Comparative Clinical Trials At Bahadur Nagar
by Sajid Mehmood | Prof.Dr.Muhammad Sarwar Khan | Dr.Muhammad Avais | Prof.Dr.Muham | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2008Dissertation note: Mastitis is a mutifactorial disease-complex resulting from pathogenic- host-environment interaction. The local information on the epidemiological dimensions of mastitis in Pakistan is extremely inadequate. This information is imperative for planning an intervention strategy for this costly dairy disease. All immunization procedures developed against the disease have been remained unsuccessful due to multiple etiological agents. The aim of present project was to study the incidence of sub-clinical and clinical mastitis in pregnant and non pregnant cattle at Livestock Experimental Station, Bahadur Nagar and to conduct the comparative clinical effectiveness of parentral and intramammary treatment routes. The incidence of clinical mastitis at Bahadur Nagar, Okara was 18.6 % (56/300) and incidence of sub-clinical mastitis was 3 1 .6% (89/300). Research was conducted to determine the association of some host, management and housing determinants with clinical mastitis in cattle. Analysis of data showed that the relative risk for clinical mastitis increased with the increment of age. Similarly the chances of occurance of mastitis increased with increase in lactation number and stage of lactation. The length of dry period during the preceding gestation was inversely proportion to the risk for clinical mastitis.
The managemental data showed that risk factor for mastitis was maximum with calf stimulus. Teat dipping found to be an important measure to prevent from mastitis..
When housing data was analysed it was found that risk was maximum for the animals kept on brick floor and minimum for those kept on katcha floor. Risk for clinical mastitis was higher in animals kept with uneven surfaces. The relative risk gradually decreased when the frequency of dung removal increased.
The misuse of antibiotics at low level over longer period may cause the therapeutic failure and the development of drug resistant (uni-or multi-resistant) strains of staphylococci and other bacteria. Therefore, regular studies on the antibiotic sensitivity of the bacterial isolates are needed for the effective and economical treatment of the disease. For this purpose milk sample was examined by Whiteside test. Clinically positive naturally infected animals was divided into four groups A, B, C and D. Group A, B, C was treated with Enrofloxacin (Encure-l0, Nawan Laboratories, Karachi) l/M, Enrofloxacin (Encure-l0, Nawan Laboratories, Karachi) 1/Mm, Enrofloxacin (Encure-l0, Nawan Laboratories, Karachi) I/M and I/Mm respectively and group D acted as positive control. Another group E, comprised of healthy non infected animals acted as negative control. The efficacy of drugs was evaluated on the basis of clinical recovery of the animals and Whiteside test pretreatment and post treatment results. It was found that best route of treatment was intramammary route. It was also found that clinical as well as sub-clinical mastitis was more prevalent in non pregnant but lactating animals than that of pregnant animals.
Availability: Items available for loan: UVAS Library [Call number: 1009,T] (1).
8.
Effect Of "In Process Quality Control "Factors On Efficacy Of Bird Flu Vaccine
by Saeed Khan | Prof.Dr.Khushi Muhammad | Prof.DR.Irshad Hussain | Prof.Dr.Muham | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2008Dissertation note: Bird Flu virus was recovered from lungs, trachea, spleen and fecal contents of the infected birds in 10 days old chicken embryos. HA activity and biological titer of the virus improved by serial passages in the 10 days old chicken embryos. This could be due to high rate of mutation of the Bird Flu virus with its successive passages. Formaldehyde and binary ethylenimine (BET) effectively inactivated the virus. However formaldehyde inactivated virus showed mitigation in HA activity during storage. The BET 5mM inactivate the virus with in 16 hours of incubation at ambient temperature (25°C) or 37°C. It has minimal detrimental effect on the HA activity of the virus, even during storage at refrigeration temperature.
Bird Flu virus vaccines without adjuvant induced poor antibody response in the vaccinated broilers. The vaccine containing aluminium hydroxide gel induced antibody response that reached at peak level on 1 8 days post priming and decline thereafter. The vaccine containing montanide (oil based vaccine) increased (90.5 GMT) up to 42 days of age. Boosting of the birds primed with gel based Bird Flu virus vaccine improved the production of antibody titer, while boosting of birds primed with oil based Bird Flu vaccine showed undetectable effect. This was due to increasing trend of antibody titer in oil based primed birds. Montanide based vaccines are therefore recommended for broiler, layers and breeders in high risk area of the disease.
Vaccines containing decreased infectivity titer induced decrease antibody titer in the vaccinated broilers. Bird Flu virus improved its HA activity and infectivity titer with serial passages in 10 days old chicken embryos. It is worth mentioning that serial passages of the virus tremendously decreased its antigenicity. It is therefore recommended to prepare commercial vaccine fom passage number 1-4, for effective immuno-prophylaxis.
The Bird Flu virus (H5N1) mutates very rapidly every time it passes through chicken embryos. It is therefore suggested to grow the virus at least in bio-safety level-ll plus (BSL-II +) laboratories.
On account of it high rate of mutation and risk of human health hazards it is suggested that Bird Flu virus (H5N1) may not be used in vaccine production, however other serotypes containing H5 and N antigen other than N1 may be used for production of commercial vaccine.
Availability: Items available for loan: UVAS Library [Call number: 1013,T] (1).
9.
Detection And Taxonomy Of Zoonotic Soil-Borne Helminths Of Cats And Dogs In Lahore
by Farhan Basit | Dr.Kamran Ashraf | Prof.Dr.Azhar Maqbool | Prof.Dr.Muham | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2009Dissertation note: To find out the prevelance of parasitic eggs/larvae of zoonotic importance, 160 faecal samples, 80 soil samples and 80 herbage samples from each of the selected park during four months i.e. from 1St June to 30th September 2007 were collected and examined. The public parks included; 1) Nasir Park, 2) Iqbal Park. Thus in total 640 samples were examined for prevelance of parasitic eggs/larvae of zoonotic importance including faeces, soil and herbage. At the same time 160 faecal samples of pet dogs and cats were collected from the Pet Centre of University of Veterinary and Animal Sciences, Lahore and were examined for the presence of zoonotic parasites. These samples were processed and examined according to the techniques i.e. direct smear method, flotation and Baerman techniques. Five samples of each material were collected every week. The effect of meteorological data was also studied. Overall infection of the parks showed that 118 (37%) out of 320 faecal samples, 44 (27.5%) out of 160 soil samples and 81(51%) out of 160 herbage samples were found positive for parasitic eggs/larvae whereas 243 (38%) out of total 640 samples were found positive for parasitic contamination. One hundred and eighteen (37%) out of 320 faecal samples were found positive in which 72 (61%) were positive for A ncylostoma caninum, 23 (19%) for Toxocara canis, 15 (13%) for Diplidium caninum and 8 (7%) were found positive for others. Out of 160 soil samples 44 (27.5%) were found positive for parasitic infection. Amongst these 31 (70%) were positive for Ancylostoma caninum, 9 (20%) were positive for Toxocara canis and 4 (10%) were positive for other nematodes. Eighty one (51%) out of 160 samples was positive for parasitic infection. Amongst these 48 (59%) were positive for Ancylostoma caninum, 21 (26%) for Toxocara canis and 12 (15%) for others were found positive. The total 243 (38%) out of 640 were found positive for parasitic contamination. Amongst those 53 (22%) were positive for Toxocara canis, 151(62%) for Ancylostoma caninum, 15 (6%) for Diplidium caninum and 24 (10%) for others including Tinia saginata, Strongyloids etc. To find out the prevalence of zoonotic parasites in pet dogs and cats 160 faecal, 80 for dogs and cats each, samples were collected form the pet centre of University of Veterinary & Animal Sciences, Lahore. Among dogs 25 (31%) out of 80 faecal samples were positive for parasitic eggs/larvae and while in cats 23 (29) out of 80 samples were positive for parasitic eggs/larvae. Overall 48(30%) out of 160 faecal samples were found positive for parasitic infection.
Availability: Items available for loan: UVAS Library [Call number: 1046,T] (1).
10.
Effect Of Types Of Floor And Bedding On Growth Performance Of Sahiwal Calves During Winter Season
by Muhammad Ali Hasni | Muhammad Jalees Ahmad Bhatti | Mr.Khurshid Ahmad | Prof.DR.Muham.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2007Dissertation note: Traditional calf management system prevailing in the villages is leading to poor immune system and occurrence of several diseases, like white scour, pneumonia, ultimately resulting in to death of calves. Early calf mortality is a major problem at livestock farms. Housing management experiment was conducted at "Livstock Experiment Station, Fazilpur, Rajanpur to investigate the effect of types of floors and beddings on performance of Sahiwal calves during winter season. Thirty six Sahiwal female calves of 84.44 kg initial body weight were fed for twelve weeks on green fodder ad-libitiun2 and concentrate @ 1% of body weight, daily. The data on DM1, weight gain, body measurements, hoof and skin health, sitting behavior and on health problems were collected.
The daily DM1 in calves kept on Katcha floor (A), concrete floor (B), on rice straw bedding (C) and sand bedding (D) treatments was 2.99± 0.01, 2.97± 0.01, 2.77± 0.01 and 2.66± 0.01 kg, respectively. Statistically highly significant difference (P<0.05) was observed between treatments. The calves on treatment A, B, C and D gained 0.409± 0.01, 0.373± 0.01, 0.404± 0.01 and 0.427± 0.01 kg daily, respectively.
Hooves length recorded in calves on treatments A, B, C and D was 0.19± 0.004, 0.16± 0.004, 0.20± 0.004 and 0.19± 0.004 cm, respectively. Hoof width data was significantly (P< 0.05) different between treatments. The body height in calves on treatment A, B, C and D was 1.21±0.07, 1±0.07, 1.03±0.07 and 1.12±0.07 inches, respectively. The highest (5.23±0.14 inch) body girth measurements were recorded in calves on D followed by C, A and B treatments, respectively. Body length measurements in calves on treatment A, B, C and D were 2.99±0.08, 3.04±0.08, 3.23±0.08 and 3.09±0.08 inches, respectively. Significant differences (P<0.05) were observed in body height and girth of calves between treatments.
The calves kept on concrete floor showed some skin eruption on their thigh region and lower part of belly. This may be due to high rate of friction and wetness, because concrete floor did not properly and completely dry due to its non absorptive ability. The calves kept on Katcha floor, Sand bedding and unchaffed paddy straw, showed no skin lesions due to good absorptive ability and very low friction. Observations regarding health problems indicated that about 45% calves on different treatments showed diarrhoea problem. The calves on concrete floor were more susceptible to diarrhoea due to winter season effect.
The calves showed a maximum sitting time of 744 mm under treatment C (managed on concrete floor + Unchaffed paddy straw bedding) and lowest sitting time (611 mm) was observed in the calves on treatment B (concrete floor). The calves showed highest (9.17 times) sitting frequency on treatment C (rice straw bedding). The lowest sitting frequencies were observed in the calves on D (sand bedding) treatment. The calves seem to be more comfortable on deep rice straw bedding (C) due to high temperature and softness of straw bedding during winter season than calves on other treatments.
The calves on treatment A (managed on Katcha floor) were always neat and clean due to the dryness of the floor and they looks good and attractive appearance, while the calves on B (managed on Concrete floor) showed very dull and dirty appearance and also suffer from skin eruptions on their lower part of the belly and thigh region. The calves on C (concrete floor with deep rice straw bedding) and D (concrete floor with sand bedding) treatment also look clean and attractive.
CONCLUSIONS
It is concluded that the performance of calves on treatment C (concrete floor+ deep straw bedding) and A (on katcha floor) was best than on other treatments (concrete floor and sand bedding). Better performance of Sahiwal calves can be attained in terms of increased DM1, weight gain, body measurements, and prevention from excessive wear & tear of hoof and providing them comfortable site for easy sitting and for good and shinning appearance during winter season by managing them on Katcha floor and/or on concrete floor alongwith application of deep rice straw bedding.
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11.
Helminths In Peafowl (Pavo Cristatus) At Lahore Zoo
by Abdul Basit | Dr.Muhammad Sarwar Khan | Dr.Jawaria Ali Khan | Prof.Dr.Muham | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2009Dissertation note: The study was conducted to determine the infection rate of helminths in peafowl and to evaluate the comparative efficacy of two different broad spectrum anthalmintics.
For this puiose, eighty seven different breeds of peafowl like Blue peafowl, Java green peafowl, White peafowl, Black shoulder peafowl, Emerald peafowl and Pide peafowl of different ages, both male and female were selected which were present at Lahore Zoo. Faecal samples of 87 birds were collected in clean polythene bags, properly labeled and examined for the identification of helminths in the laboratory of Clinical Medicine & Surgery department, University of Veterinary & Animal Sciences Lahore. Faecal samples were examined by direct smear and centrifugal floatation methods for qualitative examination. For quantitative examination McMaster Egg Counting technique was used.
Moreover, chemotherapeutic trials were conducted by making four groups of birds. Albendazole (Methyl [5-(Prophylthio)-IH-Benzimidazole-2-yl] carbonate) (Selmore Pharma) at the dose rate of O.lml per k.g. of body weight in drinking water and Pyrantel Pamoate (1 Methyl-2-(2[2-thienylj-l, 4, 5, 6-tetrahydropyrimidine, 4, 4-methylenebis (3- hydroxy-2-naphthoic acid) (Pfizer) at the dose rate of O.lml per k.g. body weight oral suspension only once in the experimental trial was used and there percentage efficacy was determined. Drugs were administered orally to each bird using crop needle.
After the collection and laboratory examination of pre-medicated eighty seven faccal samples, the positive birds of different breeds of peafowl were divided in to three equal groups that were A, B and C. Forty nine out of 87 birds were positive for single or mixed infection of Heterakis gallinae, Ascaridia gaul, Davenia pro glotina, Capillaria columbae and Acuaria spiralis with there individual percentage was 36.73%, 26.53%, 6.12%, 18.37% and 12.24% respectively. Out of 49 birds 48 positive birds were divided in groups, each group consisted of 16 birds respectively. Group 'A' consisted of (Blue peafowl, Java green peafowl, White peafowl, Black shoulder peafowl and Emerald Peafowl, Pide peafowl). Group 'B' consisted of (Blue peafowl, Black Shoulder peafowl, Emerald peafowl and White Peafowl, Java green, Pide peafowl). In group 'C' birds included were (Blue peafowl, White peafowl, Pide peafowl and Black shoulder Peafowl, Emerald peafowl). While in group 'D' (White peafowl, Java green peafowl, Pide peafowl
& Blue Peafowl) only uninfected and untreated birds were kept.
Drug therapy was only induced to group A i.e. Albendazole 0.lml/kg body weight and group B Pyrantel pamoatc 0.lml /kg body weight, while infected but untreated birds were placed in group C.
The overall prevalence of gastrointestinal helminths in different breeds of peafowl pre-medicationwas found as 56.32 %. While the healthy birds were 43.68%. Faecal egg counts were again carried out on day 03, 07 and 10 post-medication and percentage reduction of EPG calculated. On day three the percentage efficacy of Albendazole was 44.76%, on day seven 73.01%, and on day ten 94.92% respectively. In the same way percentage efficacy of Pyrantel pamoate on day three was 29.27%, on day seven 50.13% and on day ten 78.34%.
The percentage EPG rise up to 3.75%, 6.24% and 6.99% at day 03, 07 and 10 in untreated group C was observed. While no infection was observed in group D through out the experimental study. In the current study no mortality of peafowl was found so no postmortem examination was conducted. There was no any side effect of Abendazole and Pyrantel pamoate was found in both the groups which were A & B respectively.
Availability: Items available for loan: UVAS Library [Call number: 1053,T] (1).
12.
Isolation And Identification Of Staphylocococcus Aureus And Salmonella From Snack Food
by M.Rizwan Saifullah | Prof.Dr.Mansur-ud-Din Ahmad | Dr.Aftab Ahmad Anjum | Prof.Dr.Muham | Faculty of Veterinary Sciences.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2009Dissertation note: The present study was planned to investigate microbial load in ready to eat foods (snacks) available at various places in and around Lahore. A total of 60 snacks containing 14 sandwiches, 20 pizza and 26 burgers were procured from various retail outlets. The samples collected were carefully packed in a clean sampling bag and processed in the microbiology laboratory at UVAS following standards protocols.Each sample was processed for APC, Coliform count, Staphylococcus aureus count and the presence of Salmonella. The results were, compared with the guidelines published by PHLS (Gilbert et al., 2000) for comparison with the food standards in developed countries like UK.
In the present study results showed that for aerobic plate counts of 21.4% snacks were of satisfactory quality, 22.5% snacks were of acceptable quality and 58.4% were of unsatisfactory quality. For coliform counts revealed that 72.3% snack food sample were of satisfactory quality while 23.6% samples were of acceptable quality and 4% snacks were of unsatisfactory quality. In the same way Staphylococcus aureus counts for the snack food samples, showed that 22.3% samples were of acceptable quality, 13% samples were of acceptable quality and 64.6% snack food samples were of unsatisfactory quality. However Salmonella could not be detected in any of the sample tested.
In the present study on the basis of aerobic plate counts the unsatisfactory snacks were 64.3% sandwiches, 46% burgers and 65% pizzI. While the coliform counts revealed that 7.1% sandwiches and 5% pizza of unsatisfactory quality.
The results of microbial assessment for the presence of Staphylococcus aureus in snack food indicated that 85.8% sandwiches samples were of unsatisfactory quality, 38% burger samples were of unsatisfactory quality and 70% pizza samples had unsatisfactory microbiological quality. However, no Salmonella spp could be isolated from 60 snack food samples.
Availability: Items available for loan: UVAS Library [Call number: 1067,T] (1).
13.
Development Of Ration For Increasing Milk Fat Percentage In Nili-Ravi Buffaloees
by Shiraz Nasim | Prof. Dr. Makhdoom Abdul Jabbar | Prof.Dr.Muham | Prof.Dr.Talat Naseer Pasha.
Material type: ; Format:
print
Publisher: 2008Dissertation note: The present study was designed to see the effect of different rations on the milk production as well as milk fat percentage. For this purpose 20 Nili-Ravi early lactating buffaloes were selected from the existing herd and randomly divided in to five groups A, B, C, D and E. The animals were selected on the basis of their age and stage of lactation. The group A was treated with conventional ration (control group) while group B and C were supplemented with oil @ 3 % and oil + niacin @ 15 g / day / animal respectively. While group D and E were supplemented with megalac @ 3 % and megalac + niacin (1 5g/day/animal). Green fodder was given for the maintenance requirement of the animals, while the production requirements were met through experimental rations. The research trial was continued for three months at BRI, Patokki.
Milk production was recorded daily. Milk composition was analyzed on weekly basis. The milk production among the different groups was 7.13 ± 0.59, 7.70 ± 1.30, 7.31 ± 1.02, 7.31 ± 0.81 and 7.46 ± 0.61 liters. Statistical analysis showed that the milk production in groups was similar and not significant between the groups but significant among the weeks.
Milk composition in terms of fat and its fractions (Solid Non Fat, Total Solids) protein and lactose percentage will be determined on weekly basis. The economics of milk production on various rations was estimated. The milk fat contents of groups were 5.29 ± 0.35%, 6.23 ± 0.37%, 6.31 ± 0.21%, 6.67 ± 0.33% and 7.41 ± 0.3 1%, for control (A), vegetable oil (B), vegetable oil + niacin (C), megalac (D) (rumen bypass fat), and megalac + niacin (E) groups respectively. The SNF contents of milk were 9.31±0.23, 9.27±0.10, 9.20±0.14, 9.50±0.30 and 9.24±0.16 for groups A, B, C, D and E respectively, while the TS contents were 13.81 ± 0.49, 14.78 ± 0.34, 14.88 ± 0.15, 15.49 ± 0.59 and 15.84 ± 0.40for groups A, B, C, D and E respectively. The milk protein contents of different groups were 3.24 ± 0.07, 3.27 ± 0.07, 3.32 ± 0.08, 3.27 ± 0.10 and 3.32 ± 0.19 respectively for control, vegetable oil, vegetable oil + niacin, megalac (rumen bypass fat), and megalac + niacin groups respectively. The lactose contents of milk were 4.20 ± 0.05, 4.30 ± 0.08, 4.39 ± 0.12, 4.32 ± 0.08 and 4.46 ± 0.14 for groups A, B, C, D and E respectively
Blood samples were collected on fortnightly basis. The blood samples were analyzed for the total proteins, urea, cholesterol and glucose level. Blood samples were collected from all animals of the groups. The serum total protein for different groups were 3.41±26.4, 3.14±42.47, 3.71±39.05, 3.36±35.02 and 3.17±38.07 g/l in buffaloes in groups A, B, C, D and E. The blood urea contents were 15.47±1.62, 22.03±2.26, 18.71±2.19, 19.65±1.34 and 16.60±4.59 mg/dl in buffaloes in groups A, B, C, D and E. The blood cholesterol contents were 139.79±41.47, 180.21±8.52, 164.77±7.79, 168.45±4.89 and 137.90±9.88 mg/dl in buffaloes in groups A, B, C, D and E, while the blood glucose contents were 44.96±2.66, 35.18±9.65, 43.57±3.24, 37.66±18.94 and 35.73±1.12 mg/dl in buffaloes in groups A, B, C, D and E.
Body weight gain of the animals was recorded on fortnightly basis. The weight of the animals was recorded early in the morning.
CONCLUSION
Dairy animals of high genetic merit require nutrients in addition to those produced from ruminal fermentation to achieve their potential and maintain milk quality. Fat and proteinaceous feed supplements should be designed to achieve optimal microbial efficiency in the rumen and to maximize digestibility and absorption fats and proteins from the small intestine. The degree of protection of fat supplements from ruminal metabolism should be as high as possible in order to minimize their deleterious impact on microbial metabolic activities. Fat supplements should also contain a fatty acid profile that allows the composition of milk fat to satisfy the nutritional demands of consumers and to provide the special physicochemical properties that are required for manufacturing
Buffaloes would be expected to exceed cattie in fat test by 1 to 3%, depending on breed and environmental conditions, but fat percentage of buffaloes appears to be influenced by environmental factors such as age and season of calving in about the same proportions as for cattle. The buffalo is less efficient as a milk producer, slower in reaching maturity, and subject to longer calving intervals than Sahiwal cattle in Pakistan. The high fat content of buffalo milk, coupled with larger fat globules, results in buffalo milk having higher utility on small farms than cattle milk because of ease in removal of the fat.
It is concluded that as the animals were fed concentrate ration, there is milk fat depression in the milk of dairy animals. So to maintain the milk fat for most dairy producers, the most effective method of maintaining an adequate milk fat percentage in the milk is:
o ensure that adequate fiber is in the ration, and
o include buffers in the concentrate mix or total mixed ration of the dairy animal.
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14.
The Comparative Efficacy Of Various Feed Additives On The Resistance Of Broiler To Experimenmtally Induced Cecal Coccidiosis
by Atta Subhani | Dr.Kamran Ashraf | Dr.Muhammad Lateef | Prof.Dr.Muham | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2009Dissertation note: Coccidiosis is the major parasitic disease of poultry and is caused by the apicomplexan parasites Eimeria. Drugs and live vaccines are the two main control measures of the disease; however, due to increasing concerns with prophylactic drug use and the high cost of vaccines, alternative control methods are needed. Recent evidence that various dietary and live microbial supplements can influence host immunity against enteric diseases prompted me to investigate the role of prebiotic, probiotic on coccidiosis in broiler chickens. Neem fruit (azadirachta indica) and diclazuril is also used in the experimental trial due to their anticoccidial activity. For this purpose a total of 300 day old chicks free from coccidial infection were randomly divided into six groups (A, B, C, D, E and F) each comprising 50 birds. The dietary treatments for groups A-F were basal diet only, basal diet + Diclazuril 0.5%, basal diet + lactobacillus based probiotic, basal diet + Mannan-oligosacchrides (MOS), basal diet + neem fruit (Azadirachta indica) and basal diet only, respectively. The birds in groups A-E were challenged oesophageally with 10000 viable sporulated E. tenella oocysts, while the birds in group F were remained unchallenged and serve as negative control. The oocyst per gram (OPG), clinical signs/severity of diarrhea, lesion score, body weight gain, feed consumption, FCR, mortality rates, hemoglobin estimation, total leukocyte count and differential leukocyte count were the parameters considered. The efficacy of various treatments was estimated on the basis of reduction in OPG, clinical signs/severity of diarrhea and lesion score. The effect of coccidiosis on hemoglobin, total leukocyte count and differential leukocyte count were also recorded. The data thus obtained were subjected to statistical analysis using Analysis of Variance (ANOVA) Technique. The difference among the treatment means were tested by Duncans's Multiple Range (DMR) Test.
The results of the experiment revealed that oocyst per gram (OPG), clinical signs/severity of diarrhea, lesion score, mortality rates, feed consumption, hemoglobin estimation, total leukocyte count and differential leukocyte count of group D and C were significantly lower than group A, B and E (p<O.O5) respectively except group F (-ye control), but feed consumption of group C and D is significantly lower than group F (p<O.05). Body weight gain, and FCR of group C and D were significantly higher than group F, followed by E, B and A (p<O.O5) respectively.
From the results of above experiment it is concluded that both prebiotic and probiotic not only facilitate as an anticoccidial but also helpful in body weight gain. These consequently result as an alternative drug free approach to antibiotics for the control of coccidiosis because of drug resistance as well as growth promoter and ultimately reduce the economic losses due to coccidiosis.
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15.
To Study Of Commercially Available Probiotics And Prebiotics On The Performance And Nutrient Availablity In Japanese Quail
by Agha Shehzad Shaukst | Dr.Saima | Dr. Makhdoom Abdul Jabbar | Prof.Dr.Muham.
Material type: ; Format:
print
Publisher: 2009Dissertation note: Poultry feed accounts for 60 to 70% of total cost of production. To increase the efficiency of feed, different types of growth promoter likes enzymes and antibiotics have been used. But long term use of these antibiotics has side effects like antibiotic resistance and drug residues in meat. Many countries are regulating the use of antibiotics in feed and setting up programs to reduce the overall use of antibiotics. The impending ban on antibiotics in animal feed, the current concern over the spread of antibiotic resistance genes, the failure to identify new antibiotics make a compelling cause for developing alternative prophylactics. The use of probiotics and prebiotics is recommended as an alternative to antibiotics as prophylactic, therapeutic and growth-promoting agents in poultry production.
Keeping this thing in view a 28 day long feeding trial was planned. A total of 420 day old Japanese quail chicks were randomly assigned to 7 dietary treatments each having 3 replicates of 20 birds. Seven diets were formulated in such a way that they contained similar levels of CP and ME (24% & 2900 kcal/kg) but different in Probiotics and Prebiotics levels. Diet A was ,in accordance with the nutritional requirements of Japanese quail as specified in NRC (1994) whereas, diets B, C and D contained probiotics 50mg, 75mg and 100mg respectively. Diets E, F and G contained Prebiotics ig, 2g and 3g respectively. Feed consumption, body weight gain, FCR, dressing percentage, giblet weight, plasma calcium magnesium and phosphorus, zinc retention, plasma cholesterol, economics of all experimental rations were the parameters considered. Data thus obtained were subjected to statistical analysis using Analysis of Variance (ANOVA) Technique. The difference among the treatment means were tested by Duncan's Multiple Range (DMR) Test.
The results of the experiment revealed that body weight gain, feed consumption, FCR, were significantly (p<O.O5) higher in probiotic and prebiotic added groups. Whereas plasma calcium was significantly (p<O.O5) higher in brebiotics added groups than control and prohiotics added groups. Plasma cholesterol was significantly lower in probiotic added groups. Plasma magnesium, phosphorus, zinc concentration, giblet weight and dressing percentage showed nonsignificant (p>O.O5) difference among all the groups.
Data pertaining to economics of the experiment revealed a considerably low cost of feed/kg of the group B and C. While the group D and prebiotic added groups did not decreased the feed cost.
Conclusion:
o Use of probiotics & prebiotics increased the weight gain but the effect of prohiolics was more pronounced
o Prebiotics increased the Ca absorption
o Probiotics decreased the plasma cholesterol
o Use of probiotics proved to be economical although prebiotics also improved the weight gain and FCR but it is not economical to use it as growth promoter
From the results of above experiment it is concluded that inclusion of prehiotics have no considerable benefits but the use of probiotics at low concentration is useful. This consequently results in considerably improved performance and ultimately higher profitability of the farm.
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16.
Profile Of Calcium And Phosphorus In Sheep And Goats In Different Ecological Zones Of Punjab Pakistan
by Yasir Allah Ditta | PRof.Dr.Anjum Khalique | Prof.Dr.Muham | Prof.Dr.Talat Naseer Pasha.
Material type: ; Format:
print
Publisher: 2010Dissertation note: livestock accounts for 52.2 % of agricultural value added, contributes 11 % to GDP and affects the lives of 30-35 million people in rLlral areas directly or indirectly. The sheep and goats population during 2007-2008 was 27.1 and 56.7 million heads, respectively. In spite of a large population of sheep and goats in Pakistan, a lot of hurdles are ahead regarding the production especially with nutrition. The minerals become more important because the small ruminants are mainly fed on grazing without any proper feed and mineral supplementation except the common salt, so the only source of minerals are the grasses and lorages. Availability and concentration of minerals in crops and forages are influenced by environmental fectors such as climate and seasonal conditions during the growth, the amount of rainfall, the type and the fertility of soil on which the forages grow. the stage of maturity. the genus and the species (variety) of the fbrages. It is important to determine the mineral profile based on animal fluid/tissue concentrations in order to estimate the mineral needs of grazing ruminants, as well as the time of the year when they are most needed. Ca and P represent about 46% and 29% of total body minerals. Both Ca and P are very closely related; a deficiency or an excess of one will interfere with proper utilization of the others. [he main goal of this study was to identify profile of Ca and P in sheep and goats of five canal irrigated districts of Punjab. The samples of blood from sheep and goats, water, soil, crops and feed stuffs were taken in both summer and winter seasons and their concentrations were studied For the purpose of sampling, each district was divided into five sub-locations and these were considered as representative areas of the district. Samples were taken from each sub-location, brought to laboratory tbr analysis and analyzed in Atomic Absorption Spectrophotometer and spectrophotorneter. This study was helpful in providing the information about the status of Ca and P in soil, forages, water and in small ruminants and their seasonal dynamics and variation which will be helpfti I to formulate ratiois and premixes in the future. Mineral concentration data were statistically analyzed by t-Test and One-Way Anova.
Significant difference (p<O.05) was observed in Gujaranala, Ilali, Ahad and Khushab districts and non-significant (p>O.05) difference was observed br Ca. Significant (p<O05) difference was seen for Sheikhupura and non-signilicant (p>O.05) difference for other districts between summer and winter seasons. Significant (p<O.05) difference was observed for among Rice zone. Ihal canal irrigatd zone and D. G. Khan Irrigated zone of Punjab br goat. Significant (pO.O5) difference was found among Gujranala, I-labiz Abaci and Khushah districts in both summer and winter seasons. Non-significant (p>O.05) difference was observed for Sheikhupura and Muzzafargarh districts in both summer and winter seasons. Significant (p<O.O05) difference was seen for Gujranwala. Ilafiz Abad arid MuziaIirgarh districts and non-significant (P>O.05) difference was observed for Sheikhupura and Khushab districts between summer and winter seasons. Significant (p<O.05) difference was found in the Ca level of blood plasma in the ThaI irrigated canal zone for the sheep. The highest value for Ca level in the soil was foLind in Sheikhupura (257.5 ±19.2 ppm) and Gujranwala (2 I 6.5]. 1 7.8 ppm) districts during the winter season and in I-Iafiz Abad (404.3] 14.4 ppm) and Gujranwala (304.2±21 .3 ppm) districts during the summer season. l'he overall value of Ca for summer and winter seasons was highest for Haflz Abad (285.2±16.8 ppm) and Gujranwala (260.36±10.2 ppm) districts. [he highest value for P in the soil was found in the Sheikhupiira (40.3±6.12 ppm) district followed by Haliz Abad (30.0±3.89 ppm) district during the winter season. For summer season, the highest value of P in the soil was found in the Khushab (31.516.54 ppm) district followed by Sheikhupura (30.9±6.19 ppm) district. The average value of P for summer and winter seasons was found highest for Sheikhupura (35.616.49 ppm) district and least For Muzzalhrgarh (20.44.44 ppm). 'Ihe highest For (2a concentration in tube well water was found for Muzzatärgarh (2.006fO.987 ppm) district and the lowest being in Hafiz Abad (1.018± 0.693 ppm) district and for motor pump the highest value was for Muzzafargarh (1 .593-I 0.215 ppm) and the lowest being was For HaFiz Abad (0.7231 0.3 I I ppm) during the winter season (Tab Ic 4. I 3). For summer season, the highest value of Ca was For Muzzafargarh (2.4901 0.772 ppm) district and the lowest valLie was for lIaIiz Abad (1 .0251 0.3 16 ppm) district in tube well water and for motor pump, the highest value was for Khushab (2.160± 0.20 ppm) and the lowest being was for I lafli Ahad (I . 123.1 0. 105 ppm) district (Table 4. I 3). In canal water, the highest value of Ca was found (3.238i 0.765 ppm) arid the lowest being was for Ilafiz Abad (l.768L0.Q23ppm) district (Table 4. 1 5). In hand pump, the highest value being Found was ol Khushab (I .64f0.0 I ppm) district and the lowest was of Sheikhupura (0.94210.0! ppm) district (Table. 4.16).
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17.
Epidemiology, Serodiagnosis, Therapy And Control Of Schistosomiasis In Buffloes
by Ghulam Murtaza Arshad | Prf.Dr. Azhar Maqbool | Pof. Dr. Haji Ahmad Hashmi | Prof.Dr.Muham | Faculty of Veterinary Sciences.
Material type: ; Format:
print
Publisher: 2008Dissertation note: Schistosomiasis is one of the major diseases of public health and socio-economic importance in the developing countries of the world. Among human parasitic diseases, Schistosorniasis ranks second to malaria in tern-is of world wide public health risk. Keeping in view the importance of disease, the study was conducted to record the month wise and season wise prevalence of Schistosorniasis in buffaloes in four districts of the Punjab, province ie., Lahore, Sargodha, Kasur and Sheikhupura. The present study comprises of four parts.
Part I: deals with epidemiology of Schistosomiasis in buffaloes. Overall prevalence of Schistosomiasis in buffaloes, different farms of Punjab province indicated that infection was the highest (17%) at Kasur district followed by Sheikhupura (14.83), then Lahore (14.6%) and the lowest (13.66%) at Sargodha. The highest month wise prevalence was recorded during August (25.5%) followed by July where as the lowest during December and January.
Infection in buffaloes was higher in animals over two years of age (1 9%) than animals below two years of age (5%) in all the four districts of Punjab. The prevalence was higher in females (15.98%) than male (9.48%). There is variation in the prevalence as there is difference in the environmental and managemental condition of the area. For the serodiagnosis i.e. ELISA was used, the results indicated that the prevalence was lesser than the faecal examination because this was more specific and sensitive than the faecal examination.
Part 2: deals with the prevalence and ecology of snails. Various species of snails which act as the intermediate host of the Schistosomes were collected from the study area. The e of infection in the snails and role of cercariae in transmission of the disease was studied. A total of 10418 snails were collected of these 13.51 per cent were found to be infected. Among these 2350 were collected from Kasur district with infection rate of 14.51 percent followed, by Sheikhupura 2882 (13.6%) then Sargodha 2709 (13.40%) and the lowest at Lahore 2477 (12.51%). At Kasur district, genus wise prevalence of snails with infection rate indicated that Oncomelonia, indoplanorbis and Bullinus are the predominant genera with infection rate of 31.79, 17.10 and 14.46 percent respectively. However the highest number of the snails collected belonging to the genera Indoplanorbis. At Sheikhupura district, genus wide prevalence of snail indicated that Bullinus, Lymnaea, Indoplanorbis and Physa are the four prominent snails with infection rate of 24.74, 20.57, 14.66 and 13.84 percent respectively. At Sargodha district, genus wise prevalence of snails indicated that Lymnaea, Indoplanorbis, Bullinus and Physa are the four prominent snails with infection rate of 25.09, 14.29, 14.28 and 16.77 percent respectively. At Lahore district, genus wise prevalence of snails indicated that Bullinus Lymnaea, Physa and Indoplanorbis are the four prominent snails with infection rate of 23.37, 18.96, 13.97 and 12.70 percent respectively.While the prevalence at the snail level the Chi square value is 242.944 and the P-Value is 0.0000 1 which is highly significant.
Part 3: deals with the meteorological data ie, temperature, humidity, rainfall and pan evaporation with prevalence of snails and parasites. The temperature and rain fall play very important role in the spread of disease. The ideal temperature ranges form 22-25 °C where development within snail takes place in an efficient manner similarly humidity
f ranges from 55-70% is ideal for the development of the snail and the parasite. Rainfall is very important for the spread of the disease. There is a positive correlation of disease incidence to maximum and minimum temperature, humidity, and rainfall and pan evaporation. It was seen that during summer and autumn, optimum temperature, relative
humidity and rainfall play an important role for rapid propagation of the parasitic life
Part 4: deals with therapeutic trials against Schistosomiasis in buffaloes. A total of 150 animals (140 infected and 10 animals, normal) age ranged 5-9 years and of both sexes naturally infected with Schistosorniasis were used in thirteen controlled experiments. The efficacy of certain indigenous drugs, including Nigella sativa (Kalongi) , Caesalpinia
Crista (Karangwa), Lagenaria siceraria seeds (Kadoo ke Beej), Sausseria lappa (Qushte-e-Shreen) and Praziquanlel was compared with each other and control. Efficacy was quantified by determining the difference of egg per gram faeces (EPG) pre and post treatment. After the single dose of 50, 75 and 100 mg 1kg body weight of Nigella sativa (Kalongi) reduced EPG by 65.85, 68.29 and 71.79 per cent, respectively. After the second dose the respective reduction in EPG was 85.36, 92.68 and 94.87 percent. Caesalpenia crista at three dosage levels i.e.50, 75 and 100 mgI kg body weight caused 46.34, 53.65 and 59.52 percent reduction respectively while the reduction in EPO after second dose was 82.92, 90.24 and 92.85 percent respectively. Lagenaria siceraria Seed at three dosage levels i. e., 50, 100 and 150 mg/ kg body weight caused the reduction in EPG reduction 47.61, 52.63 and 64.10 percent respectively, while after second dose, counts as the reduction 80.95, 86.84 and 92.30 percent respectively. Sausseria lappa at three dosage levels i.e., 100, 150 and 200 mg/ kg body caused EPG reduction as under 50.00, 53.48 and 56.09 percent respectively, while after second dose the reduction in EPG count was 71.42, 81.39 and 85.36 percent respectively. Where as Praziquantel at the dose of 10 mg/body weight caused reduction in EPO 66.66% while after the second dose the reduction in EPG count was 97.43 percent. The efficacy order was Praziquantel, Nigella saliva, Caesalpinia crista, Lagenaria siceraria and Sausseria lappa. No side effects with any drug were noted. All animals showed clinical improvement after the treatment.
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18.
Preualence And Chemotherapy Of Nematodes In Pigeons At Lahore Zoo
by Sumaira Maitla | Prof.Dr. Azhar Maqbool | Dr.Muhammad Lateef | Prof.Dr.Muham.
Material type: ; Format:
print
Publisher: 2009Dissertation note: Pigeons came from South Asia where they have selected for their flight ability. During the last 150 years, they have periodically been released in several sites as potential game birds but without success since they migrate away from the release point (Williams, 2000).
The present study was carried out at Lahore Zoo and University of Veterinary and Animal Sciences Lahore and postgraduate laboratory UVAS, Lahore. To record the prevalence of various nematodes of Pigeons, a total of 150 faecal samples were collected (75 reared at floor and 75 in cages) under different managemental conditions. Faecal samples were examined by three different techniques i.e. Direct smear, Flotation and Mc Master Egg counting technique. Out of 150 samples 33.5%) pigeonss were positive in cage system and (37.5%) were positive on floor system. Overall prevalence in both the systems of management was (35.5%).
Chemotherapeutic trails were conducted by using Albendazole and Mebendazole. For this purpose a total of 45 birds from the 105 positive birds were selected. These were randomly divided into three groups i.e. A, B and C having 15 birds in each group. l3irds in group A and B treated with Albendazole and Mebendazole. At their recommended dose rate respectively whereas birds in group C were not given any treatment. The efficacy of Albendazole and Mebendazole was 97.54% and 82.68% respectively.
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19.
Different Appositional (Anastomotic) Techniques For The Repair Of Ruptured Ureter In Dogs
by Asim Riaz | Prof.Dr.Muhammad Arif Khan | Mr.Shahan Azeem | Prof.Dr.Muham.
Material type: ; Format:
print
Publisher: 2011Dissertation note: The ureters are the thick, long 'tube& that aid the urine in moving from the kidneys to the bladder. They are about 10 to 12 inches in length and the urine moves downward by gravity and peristalsis (waves of contractions). The ureters enter the urinary bladder at an angle to help prevent any backflow (reflux) of urine back into the ureter. The walls of the ureter contain
I smooth muscles. The ureter enter the bladder through the detrusor muscle in the trigone region of I the bladder. The ureters course oblique for several centimeters through the bladder wall. The normal tone of the detrusor muscle in tends to compress the ureter, thereby preventing back flow I of urine from the bladder when pressure build up in the bladder during micturation or bladder compression. The backflow of urine is prevented by valves known as ureterovesical valves. Any disease condition of ureter like ectopic ureter or devitalization due to foreign body necessitates the resection of it. This project has been designed to evaluate different suturing techniques like end-to-end anastomosis,slanting and telescoping. The study has been designed on twenty four stray dogs. The dogs will be divided into 3 groups i.e group A, B, and comprising 8 animals each. The dogs of group A will be numbered from 1-8, group B from 9-16, group and C from 17-24 for the identification purpose. The surgery will be conducted through ventral midline celiotomy from umbilicus to a variable distance caudally under general anesthesia. In group A,
Slanting, in group B, end to end anastomosis and in group C Telescoping technique will be practiced. Simple interrupted suturing techniques with 3-0 Poly galactin 910 (Vicryl) will be performed . In group A, operated dog No.5 died on day 15 post operatively. The versatility and comparison of the techniques were evaluated on the basis of health status including vomiting, diarrhea, and weight gain or weight loss, urographic assessment, of the anastomotic site in all three group A, B and C. The leakage was checked at 15, 30, 45,75 and 90 days post surgery using contrast urographs. During the study period the dogs were housed in the kennels available at Surgery Section, Department of Clinical Medicine and Surgery, University of Veterinary and Animal Sciences, Lahore.
The present project is clearly indicated that slant suturing technique was found to be more suitable and compatible anastomotic technique with excellent clinical superiority. It resulted in the better minimal leakage at the anastomotic site ,satisfactory is simple to duplicate under field conditions and minimal urological complications.
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20.
Comparative Study Of Differenr Treatment Methods For Improving The Nutritive Value Of Rice Husk
by kausar zeb | Prof. Dr. Makhdoom Abdul Jabbar | Prof.Dr.Anjum Khalique | Prof.Dr.Muham.
Material type: ; Format:
print
Publisher: 2010-2011Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1255,T] (1).
21.
Comparative Evaluation Of Various Canine Parvovirus Vaccine In Dogs
by Muhammad Usman Asghar | Dr.Aftab Ahmad Anjum | Prof.Dr.Masood Rabbani | Prof.Dr.Muham.
Material type: ; Format:
print
Publisher: 2011Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1269,T] (1).
22.
Genetic Diversity Analysis Of Sahiwal And Dhanni Cattle Breeds By Cytochrome B Gene And Microsatellite Markers
by Zahoor Ahmed | Prof.Dr.Masroor Elahi Babar | Mr. Tanveer Hussain | Prof.Dr.Muham.
Material type: ; Format:
print
; Nature of contents: ; Literary form: Publisher: 2010Dissertation note: Pakistan has various dairy breeds of cattle but the genetic data of different cattle breeds including Sahiwal and Dhanni is lacking which need to be established for their genetic identification. Blood samples of unrelated true representative of breeds (Sahiwal and Dhanni) were collected from their respective home tracts and different Government livestock farms. DNA extracted with the standard protocol (Inorganic Method) in Molecular Biology and Genomic Laboratory, Institute of Biochemistry and Biotechnology (IBBT), University of Veterinary and Animal Sciences, Lahore. Nine fluorescent dye labeled microsatellite markers having high polymorphism information content (PIC) values were used and genotyping was done. These results were analyzed statistically by softwares "POPGENE 1.31 and POWER STAT" 2.1. Allele frequency, heterozygosity, homozygosity, polymorphism information content (PIC), power of discrimination, power of exclusion, F-Statistics and Gene Flow for all Loci, population's dendogram, Nei's genetic identity and genetic distance/ diversity were calculated. The average observed heterozygosity was 0.5845 and 0.5911 in Dhanni and Sahiwal respectively. The mean observed homozygosity was 0.4155 and 0.4089 in Dhanni and Sahiwal respectively. The average PIC (Polymorphic Information Content) values of nine loci showed by Dhanni and Sahiwal cattle are 0.61 and 0.77 respectively. Genotyping results of microsatellite markers were clearly different for two different breeds showing a distinct genetic distance between Dhanni and Sahiwal cattle breeds. For further confirmation of the breeds amplification
of the mitochondrial Cyt b gene was done with especially designed primers which were designed by using Primer3 software. Sequencing of PCR fragments was done. Analysis of the sequences was performed by multiple sequence alignment with the help of Blast 2sequence and BioEdit soft wares. Identified SNPs were analyzed and haplotypes were formed. Phylogenetic tree was constructed by MEGA 4.1.
The use of genetic markers provided the information on population genetic structures of the indigenous cattle breeds even if they lack detailed pedigree recording data. The study on the genetic diversity showed the differentiation of breeds and individual breeds have unique combinations of genes as a result of phylogenetic tree. This work will provide the genetic data which will be helpful in breed identification and making effective breeding policies and conservational activities in Pakistan in future according to FAO global Farm Animal Genetic resource data.
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