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Comparative Efficacy Of Immunomodulators Seleniu, Vitamin E And Herbal Supplements Against IBD Vaccinated Broiler Chickens

by Beenish Zahid | Dr.Muti-ur-Rehman | Prof.dr.Muhammad Younas Rana.

Material type: book Book; Format: print ; Literary form: not fiction Publisher: 2011Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1248,T] (1).

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Comparative Efficacy Of Rocombinant Avian Influenza H9N2 Vectored Vaccine In Poultry

by Nadia Mukhtar | Prof.Dr.Masood Rabbani | Dr.Tahir Yaqub | Prof.Dr.Muhammad Younas.

Material type: book Book; Format: print Publisher: 2011Dissertation note: A correlative study testing the impact of different urban congestions in Lahore upon the bird abundance and diversity was conducted in the monsoon and winter season. A platform of 6 feet diameter and 4 feet height, with a variety of seeds, was offered to attract the grainivorous birds. The number and variety of birds visiting the feeding station was noted, from Dawn to dusk after a period of 6 day installation, which depicted the bird's urbanization in the area. A significant increase in the abundance of birds was observed in the winter season. Areas with large sized pockets of vegetation supported the largest diversity of birds. Grain preference of the birds recorded in the different areas studied showed a relationship between the age long practices of feeding birds with the habituation of the birds. In order of Preference Birds of most areas preferred Pearl Millet (Bajra) seeds, and Rice Seeds, over others. The next choice of food was shown to be Italian Millet and Red Italian Millet, when the amount of these decreased birds started feeding on, Sunflower, then Safflower, and Wheat. Mustard, corn and Chick peas remained feed of least preference. A weak correlation was found between urban population congestion and the number of pigeons, doves, babblers, house sparrows and silver bills. These birds are considered to be urbanized. As shown in the Tables the results suggest that more grainivorous species and invasive species such as House Sparrows are present in the central parts of the city. The outskirts of Lahore, the sub urban areas enjoy a large diversity of avifauna. This finding is supported by Mathew and Naik (1993). Areas where a considerable amount of vegetation is present, such as parks, gardens or big lawns with a lot of vegetation provide a good habitat can be considered as "Pockets of Wilderness" These areas, provide food and habitat for a variety of bird species. Although an exponential increase in urban congestion of cities and a need for further development does not allow room for large areas supporting natural vegetation. However, a plan has to be developed which would ensure a set amount of area for vegetation, to support a healthy diversity of bird species. Availability: Items available for loan: UVAS Library [Call number: 1265,T] (1).

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Studies On Molecular Diagnosis And Pathogenesis Of Canine Parvovirus

by Akbar Ali | Dr.Muti-ur-Rehman Khan | Prof.Dr.Muhammad Younas Rana.

Material type: book Book; Format: print ; Nature of contents: biography; Literary form: Publisher: 2011Dissertation note: Canine parvovirus, caused by a haemagglutinating CPV, is one of the most important acute viral infectious diseases of pups, had been prevalent in the country. In the present study, 50 faecal samples, from clinically suspected cases of parvovirus diseases dogs were collected from pet center of UVAS, Lahore. All the samples collected from the infected dogs were analyzed for complete blood count. There were decrease in the value of certain blood parameters including TEC, TLC, PCV. ESR ,Hb, Plt, MCV,MCH,MCHC. The samples were diluted and centrifuged to collect the supernatant. Being a haemagglutinating (HA) virus, the pre-filtered supernatant from all suspected samples was checked for HA activity using 1% washed chicken erythrocytes. Out of 50 samples, 35 samples were found HA positive. All the HA positive and negative samples were processed for extraction of viral DNA with Genomic DNA purification kit. The net isolated DNA samples were subjected to Polymerase Chain Reaction (PCR) by using specific primers (developed against the variable genomic region of the capsid protein of the DNA) of the CPV. One of the selected primer pair amplified the genomic region present in the CPV-2b. Optimization of PCR was done for the molecular level daignosis of canine parvovirus and this technique was previously not available or standardized in local conditions of Pakistan. With the use of primer pair, best results were found on following optimizing conditions like annealing temperature 55 0C, primers concentration (reverse and forward) 30 pmoles, Magnesium Chloride concentration 2.5mM, DNA (Template) volume 5 µl, Taq DNA polymerase (12.5U) and 200 µM of each dNTPs. The PCR product was analyzed for the banding pattern of 681 bp amplified by the primer pair against the standard DNA ladder (100bp) by using horizental agarose gel electrophoresis. Four of the HA negative samples were amplified by the PCR reaction. and the presence of CPV 2a and CPV 2b in Pakistan as the prevalent pathogenic strains. This study has been a first step for the molecular diagnosis of canine parvovirus in local conditions of Pakistan. It is hoped that this study will pave the way for further advanced studies on this topic. Availability: Items available for loan: UVAS Library [Call number: 1270,T] (1).

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