000			03169nam a22002057a 4500
005			20170919101359.0
008			170919b2017 xxu||||| |||| 00| 0 eng d
041			_aeng
082			_a2863-T
100			_aMuhammad Mazhar Munir (2010-VA-179) _935479
110			_c Dr. Muhammad Ovais Omer _95036
245			_aNephroprotective Effect Of Nifedipine Against Lead Toxicity In Mice
260			_c2017.
300			_a52P.;
502			_aThis study was designed to evaluate the nephroprotective effect of nifedipine against lead toxicity in mice. Exposure to lead can induce kidney damage, which is related to induction of oxidative damage and disturbance of intracellular calcium homeostasis. Twenty mice, weighing 20.0 ±2.0 g were selected for the experiment. Twenty mice were divided randomly into four groups having five mice in every group as follows: control, lead, low-dose nifedipine and high-dose nifedipine. Mice of the low- dose and high-dose nifedipine groups were given nifedipine perorally at 10 and 20 mg/Kg/day, respectively. While the mice of the lead and control groups were administrated perorally with isovolumic saline. The mice of the low-dose nifedipine, high-dose nifedipine and lead groups were injected intraperitoneally with lead acetate 40 mg/Kg/day after treatment with nifedipine. Mice in the control group were injected intraperitoneally with isovolumic saline. The whole treatment period remains for ten days. The nephroprotective effect of Nifedipine was assessed by a decrease in histological damage to the kidneys and the concentration of lead in kidney homogenate. Moreover, the levels of creatinine and blood urea nitrogen in the serum were also determined. Data was examined as mean ± SEM (standard error mean). Data was statistically analyzed using one way analysis of variance (ANOVA). Statistical significance was considered at P < 0.05. The current study showed reduction in the concentration of lead in kidney homogenate in nifedipine treated groups compared to control positive. The lead concentration in kidney homogenate was less in the control negative group than control positive group. The level of serum creatinine and blood urea nitrogen was remarkably increased in the control positive group as compared to the control negative group. Moreover the treatment with nifedipine decreased the levels of creatinine and blood urea nitrogen in serum. Histopathological study of the kidney tissue also showed that nifedipine could improve the lead induced injury in mice It was concluded from this experiment that nifedipine has potential to minimize the lead induced nephrotoxicity in mice, as indicated by the lead concentration in kidney homogenate, serum creatinine and blood urea nitrogen levels and histopathological examination. It is, therefore recommended that nifedipine can further be investigated for nephroprotective effects against heavy metal toxicities in other animal species including livestock.
650			_aPharmacology and Toxicology _915285
700			_aDr. Qamar Niaz _917208
700			_aDr. Muhammad Asad Ali _98437
942			_cTH
999			_c13540 _d13539