000			02611nam a2200193Ia 4500
005			20151001134113.0
008			150525s2009 xx 000 0 und d
041			_aeng
082			_a1083,T
100			_aAqeela Iqbal _96252
110			_cProf.Dr.Muhammad Ashraf _94840
245			_aEffect Of Two-Stroke Auto Rickshaw Smoke Solution On Wound Healing In Mice
260			_c2009
502			_aThe use of 3-wheel auto-rickshaws powered by a 2-stroke engine is widespread in south Asia; exhaust from these vehicles may cause different types of toxicities resulting in different pathologies. The aim of this study was to explore the association between exposure to 2-stroke auto-rickshaw smoke solution (2SARSS) and wound healing. The in vivo model of wound healing was customized to evaluate different stages of wound healing. A total of 56 mice were used of aged 4-5 weeks. After anesthetizing and hair removing from dorsum side of mice a wound of 3mm diameter was generated with the help of a sterile wound puncture. Smoke was collected in the lOOml distilled water from six 2-stroke rickshaws and then these solutions were filtered by syringe filters. 10% ointment of all the samples was prepared in Petroleum Jelly and applied to the experimental group, petroleum jelly to positive control group, no treatment was applied to negative control. Macroscopic evaluation was done by using high resolution camera photographs and measurement of wound by vernier caliper on alternate days. At the end of the study (day 12) histological evaluation was done when the control group wounds were healed. Histological examination revealed a significant delay in the outcome of re-epithelialization, dermal matrix regeneration, and maturation of collagen bundles among all 2SARSS-exposed wounds. Delayed activation of neovascularization was seen in the 2SARSS-treated groups at day 12 post-wounding. The Abbot curve, angular spectrum, and several other 3D surface parameters of reverse wound topographies revealed a highly significant reduction (p<O.OOl) in angiogenesis. These results demonstrate that application of 2SARSS causes a substantial delay in the progression of angiogenesis, resulting in delayed onset of wound healing. These observations validate the damaging effects of 2SARSS on wound healing. Thus, people who are directly or indirectly exposed to this toxic exhaust are expected to have delayed wound healing, which could result in chronic wounds.
650			_aDepartment of Pharmaoclogy & Toxicology _95037
700			_aDr.Muhammad Ovais Omer _95891
700			_aProf.Dr.Zafar _95884
942			_cTH
999			_c2799 _d2799