000			02032nam a2200193Ia 4500
005			20151001135312.0
008			150525s2010 xx 000 0 und d
041			_aeng
082			_a1091,T
100			_aSamreen Faisal _96432
110			_cProf.Dr.Muhammad Ashraf _94840
245			_aEffect On Lansoprazole On Antibacterial Activity Of Clarithromycin And Tetracyclin
260			_c2010
502			_aThe study was designed to evaluate the effect of antibacterial drugs in the presence of lansoprazole (proton pump inhibitor) to determine the inhibition of bacterial efflux pumps. Resistance through efflux pump is one of the major mechanisms of resistance in bacteria. Bacillus ,subtilis, Salmonella typhi and Escherichia coli were isolated from clinical specimen and identified by using different microbiological techniques. Bacteria were inoculated separately in nutrient agar and their susceptibility for tetracycline and clarithromycin were checked by using Kirby baur disk diffusion method and the diameter of inhibitory zones (DIZ) was measured and analyzed by Unianova. The media were inoculated with three different concentrations of lansoprazole 1X, 2X and 3X separately and the same experiments were performed with each of the lansoprazole concentrations. The experiment was performed in five replicates. The diameter of inhibitory zone of tetracycline and clarithromycin in the presence of lansoprazole was measured in millimeters with the help of a scale and compared with the previously measured diameter of inhibitory zone of bacteria in absence of lansoprazole. Results of study showed that with increasing concentration of lansoprazole diameter of zone of inhibition of antibacterial drugs increases. The data suggest that the lansoprazole dose dependently increased the zone of inhibition of antibacterial drugs in the three test organisms used in study.
650			_aDepartment of Pharmaoclogy & Toxicology _95037
700			_aDr.Aqeel Javeed _916440
700			_aDr.Sualeha Riffat _96434
942			_cTH
999			_c2828 _d2828