000			02543nam a2200193Ia 4500
005			20151005133440.0
008			150525s2010 xx 000 0 und d
041			_aeng
082			_a1276,T
100			_aShumaila Rani _97432
110			_cProf.Dr.Masood Rabbani _95742
245			_aDynamics Of Recombinant Avian Influenza Virus H9-Ha Gene Herpesvirus Of Turkey Vector Vaccine In Chicken
260			_c2010
502			_aAvian influenza (AI) is known to exist for centuries. It's primarily a disease of birds. For prevention and control of H9N2 avian influenza disease, HA gene from Pakistani H9N2 field virus isolate (PK-UDL/01/08 H9N2) cloned into a herpesvirus of turkey (rHVT/AI-H9 having full HA) at Institute for Animal Health, Compton, UK, expressing HA proteins, was given subcutaneously (in the neck). Commercial avian influenza vaccine was given to chicken through intramuscular route. On termination of the experiment, the chicks from all groups were sacrificed and their visceral organs were collected on chick-wise basis. Besides this, blood was collected for complete blood counts (CBC) and blood chemistry. Results of groups were then compared for any significant difference. The data analyses showed that in complete blood counts, there was no significant difference (p<0.05) of total leukocyte counts of different groups of chickens but heterophil percentages showed variation among different groups. Analyses of Serum chemistry results showed that glucose and protein concentration in serum varies significantly (p>0.05) among the different groups of birds. However, there was no significant difference of cholesterol levels among the groups of chickens. For determining the persistence of rHVT/AI-H9 having full HA virus in different visceral organ and in blood samples of Group A, results of the PCR showed the persistence of herpes turkey virus in leukocytes, spleen, liver, lung, kidney and heart. This project helped in evaluating dynamics of recombinant HVT containing avian influenza HA gene from avian influenza H9N2 Pakistani isolate. In future the study may be continued for further biological characterization by isolating the HVT from different visceral organ in different interval of time and fractionating the serum protein and analyzing the gamma globulin fraction in serum to measuring the humoral immune response against the recombinant vaccine.
650			_aDepartment of Microbiology _94609
700			_aDr.Tahir Yaqub _96821
700			_aProf.Dr.Masroor Elahi Babar _96616
942			_cTH
999			_c2991 _d2991