000			01875nam a2200193Ia 4500
005			20151005141737.0
008			150525s2011 xx 000 0 und d
041			_aeng
082			_a1348,T
100			_aMuhammad Awais _97885
110			_cMiss Shagufta Saeed _97886
245			_aProduction Of Alginate By Azotobacter Vinelandll By Submerged Fermentation
260			_c2011
502			_aAlginate is a linear coploymer of 1, 4,-beta-D-mannuronic acid and alpha -L-guluronic acid. They are usually obtained from brown algae which contains of D-mannuronic and L-guluronic acids. Alginates are important polysaccharides with many application in the food, pharmaceutical, textiles and paper industry. Azotobacter vinelandii is a gram negative, aerobic and free-living soil microbe which play an important role in the nitrogen cycle in nature. A. vinelandii was grown on wheat bran for the production of alginate. The optimal condition like substrate:water ratio, pH, incubation period, ionic concentration (Calcium, magnesium, sodium and phosphate ions) and addition of corn steep liquor was carried out for the production of alginate by A. vinelandii through submerged fermentation. The cell biomass and alginate concentration was determined to estimate the concentration of alginate. The alginate thus produced was estimated by determining its concentration spectrophotometrically at 546 nm. The effect of pH, incubation period, ionic concentration and addition of nitrogen source like corn steep liquor was analysed by statistical linear regression method. Alginate is an important polymer with wide applications in pharmaceutical, food and textile industry. It has also medical application in encapsulating hormones.
650			_aInstitute of Biochemistry & Biotechnology _95022
700			_aMiss Asma Waris _97824
700			_aMiss Sehrish _97887
942			_cTH
999			_c3062 _d3062