000			02795nam a2200181Ia 4500
005			20151006125957.0
008			150525s2012 xx 000 0 und d
041			_aeng
082			_a1463,T
100			_aFauzia Tajdin _98094
110			_cDr. Muhammad Adil Resheed _98095
245			_aDetermination Of Bacterial Etiological Agents ,Sensitivity Pattern And Clinical Outcomes Paediatrics Patients In pyogenic meningitis at children hospital Lahore,Pakistan
260			_c2012
502			_aBackground: Meningitis is inflammation of membranes of brain and spinal cord which are known as meninges collectively and these membranes provide protection. This study was designed to check the bacteriology and sensitivity pattern of pyogenic meningitis for their antibiotic susceptibility testing and pattern of resistance by meningitis isolates in patients suspected from meningitis is determined. It was conducted on different children suspected from meningitis in Children hospital Lahore, Pakistan. Hypothesis: By using CSF culturing and different biochemical tests bacterial etiological agents of pyogenic meningitis in children of less than 15 years was determined and their sensitivity pattern and clinical outcome was also evaluated. Material & Methods: Clinical manifestations of all patients admitted to Children hospital were examined at the time of admission and patients showing classic triads (fever, neck rigidity and seizure) their CSF samples were collected by using all necessary aseptic precautions with the assistance of trained professionals. These CSF samples were examined for their physiological, biochemical and cytological and microbiological analysis. The pathogens was isolated, identified and purified by selective culturing methods, which was subjected to active growth, during which sensitivity to different antibiotics were checked in vitro by Kirby Bauer Disk diffusion method. The sensitivity was measured by area marked by the zone of inhibition, and Clinical Laboratory Standards interpretations (CLSI). Standard limit was a key indicator towards resistance bacteria. Statistical Analysis: The collected data was analyzed by using ANOVA and Chie Square tests on SPSS software (16). Outcome: Different bacteria responsible for bacterial meningitis were isolated and antimicrobial susceptibility profile of different antibiotics against both Gram positive and Gram negative bacteria were also evaluated. The effects of different regimens of treatment of acute pyogenic meningitis were also evaluated by evaluating the improvement in clinical condition, rate of complications of disease and incidence of death due to this fatal disease.
650			_aDepartment of Pharmaoclogy & Toxicology _95037
700			_aProf Dr. Muhammad Ashraf _95038
942			_cTH
999			_c3169 _d3169