000			02532nam a2200193Ia 4500
005			20151006131921.0
008			150525s2012xx 000 0 und d
041			_aeng
082			_a1509,T
100			_aHassan Ali _98164
110			_cDr. Muhammad Tayyab _96158
245			_aBioconversion Of Wheatbran To Glucose By Gluoamylase From Aspergillus Fumigatus
260			_c2012
502			_aBackground: Glucose is produced by hydrolysis of starch. Many crops like maize, rice and wheat can be used as the source of starch. Wheat bran is an agricultural waste byproduct which can be converted to glucose using glucoamylase. Wheat bran is very cheap source for carbohydrates. It is mainly composed of carbohydrates; hemicelluloses, cellulose and starch. Glucoamylase is an enzyme that yields glucose from the nonreducing chain of amylose and amylopectin by hydrolyzing ? -1,3, ?-1, 4 and ?-1,6 linkages of starch. Glucoamylases are produced by plants, animals and microorganism. Microbes, including bacteria, yeast and fungi are major source for the production of glucoamylases. Aspergillus fumigatus is found in soil and in decaying organic matter and it has an essential role in carbon and nitrogen recycling. Hypothesis: A. fumgiatus might be a good source for the production of glucoamylase through submerged fermentation conditions. Parameters/Methodlogy: Aspergillus fumigatus was identified macro and microscopically. Enzyme production was measured by DNS method. The effects of different sources of carbon, phosphorous and nitrogen on glucoamylase production were also examined. In order to get the optimum production of glucoamylase, the effect of temperature, pH and incubation period was analysed separately. Methodology: Initially the A. fumigatus was isolated and conditions were optimized for the growth and production of glucoamylase. Production of enzyme was examined by DNS method. The effects of various carbon, nitrogen and phosphorous sources were examined on the production of glucoamylase. From the present study it was concluded that maximum production of glucoamylase can be obtained from A. fumigatus using wheat bran as the substrate at pH of 4.8, temperature of 40oC with an incubation time of three days.The use of wheat bran as substrate wheat bran for the production of glucoamylase will reduce the cost for the production of glucoamylase.
650			_aInstitute of Biochemistry & Biotechnology _95022
700			_aDr. Abu Saeed Hashmi _95240
700			_aDr. Asif Nadeem _95764
942			_cTH
999			_c3213 _d3213